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Characterization and antioxidant activity of the oligo-maltose fraction from Polygonum Cillinerve.

Carbohydrate polymers (2019-10-05)
Yu Zhou, Wuren Ma, Libo Wang, Wenjing Sun, Meng Li, Weimin Zhang, Yingqiu Liu, Xiaoping Song, Yunpeng Fan
ABSTRACT

In this study, Polygonum Cillinerve polysaccharide (PCP) was extracted, and characterized by high performance gel permeation chromatography, high performance liquid chromatography, fourier transform infrared spectroscopy, nuclear magnetic resonance and mass spectroscopy. The results showed that PCP was composed of glucose, it was α-D-glucan and the backbone of PCP was consisted of repeating units of (1→4)-α-D-Glucose. In addition, the antioxidant potential of PCP was assessed in vitro. The results showed that PCP had strong hydroxyl radical scavenging ability, some DPPH scavenging ability and good reducing power. Moreover, the results of the enzyme-linked immunosorbent assays showed that PCP at 15.625-0.975 μg/mL could significantly improve the level of superoxide dismutase and glutathione peroxidase, and could significantly decrease the level of myeloperoxidase, malondialdehyde and xanthine oxidase in macrophages. These results indicated that PCP could potentially be developed as a natural antioxidant.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
4-Chloro-DL-phenylalanine
Sigma-Aldrich
L-Rhamnose, natural sourced, 99%, FG