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A chromatin-remodeling protein is a component of fission yeast mediator.

The Journal of biological chemistry (2010-07-14)
Olga Khorosjutina, Paulina H Wanrooij, Julian Walfridsson, Zsolt Szilagyi, Xuefeng Zhu, Vera Baraznenok, Karl Ekwall, Claes M Gustafsson

The multiprotein Mediator complex is an important regulator of RNA polymerase II-dependent genes in eukaryotic cells. In contrast to the situation in many other eukaryotes, the conserved Med15 protein is not a stable component of Mediator isolated from fission yeast. We here demonstrate that Med15 exists in a protein complex together with Hrp1, a CHD1 ATP-dependent chromatin-remodeling protein. The Med15-Hrp1 subcomplex is not a component of the core Mediator complex but can interact with the L-Mediator conformation. Deletion of med15(+) and hrp1(+) causes very similar effects on global steady-state levels of mRNA, and genome-wide analyses demonstrate that Med15 associates with a distinct subset of Hrp1-bound gene promoters. Our findings therefore indicate that Mediator may directly influence histone density at regulated promoters.

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Protein A-Sepharose® from Staphylococcus aureus, lyophilized powder
Anti-c-Myc antibody, Mouse monoclonal antibody produced in mouse, clone 9E10, purified from hybridoma cell culture