MilliporeSigma
  • Home
  • Search Results
  • A phosphorylated cytoplasmic autoantigen, GW182, associates with a unique population of human mRNAs within novel cytoplasmic speckles.

A phosphorylated cytoplasmic autoantigen, GW182, associates with a unique population of human mRNAs within novel cytoplasmic speckles.

Molecular biology of the cell (2002-04-16)
Theophany Eystathioy, Edward K L Chan, Scott A Tenenbaum, Jack D Keene, Kevin Griffith, Marvin J Fritzler
ABSTRACT

A novel human cellular structure has been identified that contains a unique autoimmune antigen and multiple messenger RNAs. This complex was discovered using an autoimmune serum from a patient with motor and sensory neuropathy and contains a protein of 182 kDa. The gene and cDNA encoding the protein indicated an open reading frame with glycine-tryptophan (GW) repeats and a single RNA recognition motif. Both the patient's serum and a rabbit serum raised against the recombinant GW protein costained discrete cytoplasmic speckles designated as GW bodies (GWBs) that do not overlap with the Golgi complex, endosomes, lysosomes, or peroxisomes. The mRNAs associated with GW182 represent a clustered set of transcripts that are presumed to reside within the GW complexes. We propose that the GW ribonucleoprotein complex is involved in the posttranscriptional regulation of gene expression by sequestering a specific subset of gene transcripts involved in cell growth and homeostasis.

MATERIALS
Product Number
Brand
Product Description

Millipore
Protein A-Sepharose® from Staphylococcus aureus, lyophilized powder
Sigma-Aldrich
Anti-GW182 antibody produced in rabbit, ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution