• Home
  • Search Results
  • Feeders facilitate telomere maintenance and chromosomal stability of embryonic stem cells.

Feeders facilitate telomere maintenance and chromosomal stability of embryonic stem cells.

Nature communications (2018-07-07)
Renpeng Guo, Xiaoying Ye, Jiao Yang, Zhongcheng Zhou, Chenglei Tian, Hua Wang, Haiying Wang, Haifeng Fu, Chun Liu, Ming Zeng, Jun Yang, Lin Liu
ABSTRACT

Feeder cells like mouse embryonic fibroblasts (MEFs) have been widely applied for culture of pluripotent stem cells, but their roles remain elusive. Noticeably, ESCs cultured on the feeders display transcriptional heterogeneity. We investigated roles of feeder cells by examining the telomere maintenance. Here we show that telomere is longer in mESCs cultured with than without the feeders. mESC cultures without MEF feeders exhibit telomere loss, chromosomal fusion, and aneuploidy with increasing passages. Notably, feeders facilitate heterogeneous transcription of 2-cell genes including Zscan4 and telomere elongation. Moreover, feeders produce Fstl1 that together with BMP4 periodically activate Zscan4. Interestingly, Zscan4 is repressed in mESCs cultured in 2i (inhibitors of Mek and Gsk3β signaling) media, associated with shorter telomeres and increased chromosome instability. These data suggest the important role of feeders in maintaining telomeres for long-term stable self-renewal and developmental pluripotency of mESCs.

MATERIALS
Product Number
Brand
Product Description

Roche
TeloTAGGG Telomere Length Assay, sufficient for ≤50 reactions, kit of 1 (15 components), suitable for cell culture
Sigma-Aldrich
5-Methylcytidine, ≥99%
Sigma-Aldrich
Trichloroacetic acid, ACS reagent, ≥99.0%
Sigma-Aldrich
Trypsin inhibitor from Glycine max (soybean), solution, sterile-filtered, suitable for, suitable for cell culture