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A microfluidic biochip platform for electrical quantification of proteins.

Lab on a chip (2018-04-18)
Enrique Valera, Jacob Berger, Umer Hassan, Tanmay Ghonge, Julia Liu, Michael Rappleye, Jackson Winter, Daniel Abboud, Zeeshan Haidry, Ryan Healey, Na-Teng Hung, Nathaniel Leung, Naif Mansury, Alexander Hasnain, Christine Lannon, Zachary Price, Karen White, Rashid Bashir
ABSTRACT

Sepsis, an adverse auto-immune response to an infection often causing life-threatening complications, results in the highest mortality and treatment cost of any illness in US hospitals. Several immune biomarker levels, including Interleukin 6 (IL-6), have shown a high correlation to the onset and progression of sepsis. Currently, no technology diagnoses and stratifies sepsis progression using biomarker levels. This paper reports a microfluidic biochip platform to detect proteins in undiluted human plasma samples. The device uses a differential enumeration platform that integrates Coulter counting principles, antigen specific capture chambers, and micro size bead based immunodetection to quantify cytokines. This microfluidic biochip was validated as a potential point of care technology by quantifying IL-6 from plasma samples (n = 29) with good correlation (R2 = 0.81) and agreement (Bland-Altman) compared to controls. In combination with previous applications, this point of care platform can potentially detect cell and protein biomarkers simultaneously for sepsis stratification.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Streptavidin from Streptomyces avidinii, essentially salt-free, lyophilized powder, ≥13 units/mg protein
Sigma-Aldrich
Bovine Serum Albumin, heat shock fraction, protease free, essentially globulin free, pH 7, ≥98%
Sigma-Aldrich
N-Hydroxysulfosuccinimide sodium salt, ≥98% (HPLC)