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Cell Dissociation Protocol with Trypsin
Trypsin is frequently used in cell dissociation from adherent surfaces. We offer a wide variety of trypsin solutions to meet your specific cell line requirements, as well as protocols, troubleshooting ideas, and more.
Cell Dissociation Reagents
Enzymes like trypsin and collagenase are essential for the detachment of adherent cells and solid tissues for subculture and experiments. Recombinant trypsin, solutions containing EDTA, and non-enzymatic solutions provide stabler, gentler options for wider culture applications.
Removal of Adherent Cells from a Culture Surface Using Trypsin
Trypsin may be used to remove adherent cells from a culture surface. Cells are most commonly removed from the culture substrate by treatment with trypsin or trypsin/EDTA solutions.
Human Cardiac Fibroblasts (HCF) Culture Protocol
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
Human Tracheal Epithelial Cells (HTEpC) Culture Protocol
Technical information for working with human tracheal epithelial cells including thawing, subculturing and cryopreservation.
Human Placental Epithelial Cells (HPlEpC) Culture Protocol
Technical information for working with human placental epithelial cells including thawing, subculturing and cryopreservation
Human Mammary Epithelial Cells (HMEpC) Culture Protocol
Mammary Epithelial Cells and primary epithelial cells from other tissue at Cell Applications, your worldwide provider of human & animal cells, antibodies.
Human Chondrocytes Osteoarthritis (HC-OA) Culture Protocol
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
Human Fibroblast-Like Synoviocytes (HFLS) Culture Protocol
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
Human Aortic Endothelial Cells (HAOEC) Culture Protocol
Technical information for working with human aortic endothelial cells including thawing, subculturing and cryopreservation.
Human Carotid Artery Endothelial Cells (HCtAEC) Culture Protocol
Technical information for working with human carotid artery endothelial cells including thawing, subculturing and cryopreservation
Porcine Coronary Artery Endothelial Cells (PCAEC) Culture Protocol
Technical information for working with porcine coronary artery endothelial cells including thawing, subculturing and cryopreservation.
Human Astrocytes (HA) Culture Protocol
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
Human Epidermal Keratinocytes (HEK) Culture Protocol
Storage - Preparation for Culturing - Culturing HEK - Subculturing HEK
Human Chondrocytes (HC) Culture Protocol
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
Human Lung Fibroblasts (HLF) Culture Protocol
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
Human Dermal Fibroblasts (HDF) Culture Protocol
Storage - Preparation for Culturing - Culturing HDF - Subculturing HDF
Rat Dermal Fibroblasts (RDF) Culture Protocol
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
Human Coronary Artery Endothelial Cells (HCAEC) Culture Protocol
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
Rat Cardiac Fibroblasts (RCF) Culture Protocol
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
Canine Osteoblasts (CnOb) Culture Protocol
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
Human Epidermal Melanocytes (HEM) Culture Protocol
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
Bovine Brain Artery Endothelial Cells (BBAEC) Culture Protocol
Technical information for working with bovine brain artery endothelial cells including thawing, subculturing and cryopreservation
Bovine Aortic Smooth Muscle Cells (BAOSMC) Culture Protocol
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
Human Umbilical Vein Endothelial Cells (HUVEC) Culture Protocol
Human Umbilical Vein Endothelial Cells (HUVEC) Culture Protocol. Storage. Preparation for Culturing. Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
Canine Aortic Endothelial Cells (CnAOEC) Culture Protocol
Technical information for working with Canine Aortic Endothelial Cells including thawing, subculturing and cryopreservation
Bovine Pulmonary Artery Endothelial Cells (BPAEC) Culture Protocol
Technical information for working with Bovine Pulmonary Artery Endothelial Cells including thawing, subculturing and cryopreservation
CAI protocol - Porcine Coronary Artery Endothelial Cells (PCAEC) Culture Protocol
Technical information for working with porcine coronary artery endothelial cells including thawing, subculturing and cryopreservation
Human Osteoblasts (HOb) Culture Protocol
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
Human Brachiocephalic Artery Smooth Muscle Cells (HBcASMC) Culture Protocol
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
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