Showing 1-30 of 552 results for "

T8787

"
Jefferson Friguglietti et al.
Biomaterials, 244, 119927-119927 (2020-03-22)
Both hard material photolithography and soft lithography are widely used for patterned cell culture. Soft lithography techniques enable bioactive molecule incorporation, however complex surface modifications are required to introduce specific ligands or proteins in conventional photolithography. In this study, we...
Karen M Lammers et al.
Gastroenterology, 135(1), 194-204 (2008-05-20)
Celiac disease is an immune-mediated enteropathy triggered by gliadin, a component of the grain protein gluten. Gliadin induces an MyD88-dependent zonulin release that leads to increased intestinal permeability, a postulated early element in the pathogenesis of celiac disease. We aimed...
Aline Lueckgen et al.
Biomaterials, 217, 119294-119294 (2019-07-06)
Enzymatically-degradable materials recapitulate the dynamic and reciprocal interactions between cells and their native microenvironment by allowing cells to actively shape the degradation process. In order to engineer a synthetic 3D environment enabling cells to orchestrate the degradation of the surrounding...
Lokender Kumar et al.
Protein expression and purification, 148, 59-67 (2018-04-08)
MMP1 is an essential enzyme for tissue remodeling both in normal and pathological states. We report a method of purifying activated human MMP1 in E. coli without using urea or 4-Aminophenylmercuric acetate (APMA). Instead, a non-ionic detergent, Triton X-100, was...
Lígia Sousa-Ferreira et al.
PloS one, 6(5), e19745-e19745 (2011-05-19)
Some pathological conditions with feeding pattern alterations, including obesity and Huntington disease (HD) are associated with hypothalamic dysfunction and neuronal cell death. Additionally, the hypothalamus is a neurogenic region with the constitutive capacity to generate new cells of neuronal lineage...
Christian M Schürch et al.
Cell, 182(5), 1341-1359 (2020-08-09)
Antitumoral immunity requires organized, spatially nuanced interactions between components of the immune tumor microenvironment (iTME). Understanding this coordinated behavior in effective versus ineffective tumor control will advance immunotherapies. We re-engineered co-detection by indexing (CODEX) for paraffin-embedded tissue microarrays, enabling simultaneous...
Chao Liu et al.
Scientific reports, 7(1), 4543-4543 (2017-07-05)
Nucleus pulposus (NP) cells reside in a physiologically hyperosmotic environment within the intervertebral disc. TonEBP/NFAT5 is an osmo-sensitive transcription factor that controls expression of genes critical for cell survival under hyperosmotic conditions. A recent report on NP and studies of...
Rebecca Gentek et al.
Immunity, 48(6), 1160-1171 (2018-06-03)
Hematopoiesis occurs in distinct waves. "Definitive" hematopoietic stem cells (HSCs) with the potential for all blood lineages emerge in the aorta-gonado-mesonephros, while "primitive" progenitors, whose potential is thought to be limited to erythrocytes, megakaryocytes, and macrophages, arise earlier in the...
null
Paola Matarrese et al.
The Biochemical journal, 413(3), 467-478 (2008-04-30)
Participation of diverse organelles in the intracellular signalling that follows CD95/Fas receptor ligation encompasses a series of subcellular changes that are mandatory for, or even bolster, the apoptotic cascade. In the present study, we analysed the role of endocytosis in...
A D Sauerbeck et al.
Scientific reports, 8(1), 7677-7677 (2018-05-18)
Traumatic brain injury is a major source of global disability and mortality. Preclinical TBI models are a crucial component of therapeutic investigation. We report a tunable, monitored model of murine non-surgical, diffuse closed-head injury-modCHIMERA-characterized by impact as well as linear...
Aiqin Sun et al.
Autophagy, 13(3), 522-537 (2017-01-14)
The MAP1LC3/LC3 family plays an essential role in autophagosomal biogenesis and transport. In this report, we show that the HECT family E3 ubiquitin ligase NEDD4 interacts with LC3 and is involved in autophagosomal biogenesis. NEDD4 binds to LC3 through a...
Acrolein-activated matrix metalloproteinase 9 contributes to persistent mucin production.
Deshmukh H S, et al.
American Journal of Respiratory Cell and Molecular Biology, 38(4), 446-454 (2008)
Agnes Schröder et al.
JCI insight, 4(23) (2019-12-06)
Dietary salt consumption leads to cutaneous Na+ storage and is associated with various disorders, including osteopenia. Here, we explore the impact of Na+ and the osmoprotective transcription factor nuclear factor of activated T cell 5 (NFAT5) on bone density and...
Dilip Shrestha et al.
Biochimica et biophysica acta, 1840(1), 667-680 (2014-02-01)
Cluster of differentiation 1 (CD1) represents a family of proteins which is involved in lipid-based antigen presentation. Primarily, antigen presenting cells, like B cells, express CD1 proteins. Here, we examined the cell-surface distribution of CD1d, a subtype of CD1 receptors...
I-Chi Lee et al.
ACS applied materials & interfaces, 10(45), 38769-38779 (2018-11-06)
In vitro spherical cancer models have been widely used in cancer stem cell (CSC) research, and the ability of CSCs to form multicellular colonies is recognized as a morphological marker. However, although several spherical/colony models share a common three-dimensional (3D)...
Ritu Dixit et al.
Human mutation, 39(12), 1957-1972 (2018-08-29)
Transcription factor GATA4 is known to play crucial role during heart development, regulating expression of several other key cardiogenic factors. Various GATA4 mutations are reported in familial as well as sporadic cases of congenital heart defects (CHDs). To estimate the...
Hidetaka Tanno et al.
Science advances, 6(17), eaay9093-eaay9093 (2020-05-20)
Natively paired sequencing (NPS) of B cell receptors [variable heavy (VH) and light (VL)] and T cell receptors (TCRb and TCRa) is essential for the understanding of adaptive immunity in health and disease. Despite many recent technical advances, determining the...
Koji Abe et al.
The Journal of pharmacology and experimental therapeutics, 326(3), 983-990 (2008-06-25)
Previous reports have indicated that in vitro biliary clearance (Cl(biliary)) determined in sandwich-cultured hepatocytes correlates well with in vivo Cl(biliary) for limited sets of compounds. This study was designed to estimate the in vitro Cl(biliary) in sandwich-cultured rat hepatocytes (SCRHs)...
Virág Somogyi et al.
Acta veterinaria Hungarica, 64(4), 497-513 (2016-12-21)
Thyroid hormones (THs) and oestrogens are crucial in the regulation of cerebellar development. TH receptors (TRs) mediate these hormone effects and are regulated by both hormone families. We reported earlier that THs and oestradiol (E2) determine TR levels in cerebellar...
null
Vania Gelmetti et al.
Autophagy, 13(4), 654-669 (2017-04-04)
Mitophagy is a highly specialized process to remove dysfunctional or superfluous mitochondria through the macroautophagy/autophagy pathway, aimed at protecting cells from the damage of disordered mitochondrial metabolism and apoptosis induction. PINK1, a neuroprotective protein mutated in autosomal recessive Parkinson disease...
Zahira Fernández-Bedmar et al.
Foods (Basel, Switzerland), 8(6) (2019-06-27)
This study aimed to evaluate the biological activities of two Allium species (garlic and onion) as well as diallyl disulphide (DADS) and dipropyl disulphide (DPDS) as their representative bioactive compounds in a multi-assay experimental design. The genotoxic, antigenotoxic, and lifespan...
Haiqiong Wang et al.
Science advances, 5(2), eaav4971-eaav4971 (2019-02-23)
Injured axons undergo a controlled, self-destruction process, known as Wallerian degeneration. However, the underlying mechanism remains elusive. Using the Drosophila wing nerve as a model, we identify the ESCRT component Vps4 as a previously unidentified essential gene for axonal integrity....
Ming Shen et al.
Autophagy, 13(8), 1364-1385 (2017-06-10)
Oxidative stress-induced granulosa cell (GCs) death represents a common reason for follicular atresia. Follicle-stimulating hormone (FSH) has been shown to prevent GCs from oxidative injury, although the underlying mechanism remains to be elucidated. Here we first report that the suppression...
Stephanie Fore et al.
Science advances, 6(36) (2020-09-13)
The developing brain undergoes drastic alterations. Here, we investigated developmental changes in the habenula, a brain region that mediates behavioral flexibility during learning, social interactions, and aversive experiences. We showed that developing habenular circuits exhibit multiple alterations that lead to...
Sivaram Harendra et al.
Journal of environmental sciences (China), 25(3), 576-584 (2013-08-08)
Surfactants are soil washing agents and facilitators for subsurface remediation of hydrocarbon spills. It is important to understand the sorption and transport behavior of surfactants for enhanced soil remediation. The adsorption and desorption isotherms of cetyl trimethylammonium bromide (CTAB) and...
Na Wang et al.
Frontiers in pharmacology, 10, 1583-1583 (2020-02-11)
Chronic cerebral hypoperfusion (CCH) is a neurodegenerative disease, which induces cognitive impairments in the central nervous system (CNS). Histamine H3 receptor (H3R) is an autoreceptor involved in the modulation of neurogenesis and synaptic plasticity in the CNS. However, the role...
I-Chen Li et al.
Drug and chemical toxicology, 41(4), 459-464 (2018-01-24)
This study aimed to establish an in vitro model to confirm the efficacy of erinacine A-enriched Hericium erinaceus (EAHE) mycelia and investigate its potential adverse effects in a preclinical experimental setting, including an assessment on the oral administration of EAHE...
Marion Poirier et al.
ChemMedChem, 14(2), 224-236 (2018-12-07)
By screening a focused library of kinase inhibitor analogues in a phenotypic co-culture assay for angiogenesis inhibition, we identified an aminotriazine that acts as a cytostatic nanomolar inhibitor. However, this aminotriazine was found to be completely inactive in a whole-kinome...