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Mao Shen et al.
Nanoscale research letters, 9(1), 296-296 (2014-07-06)
Preparation of magnetic nanoparticles coated with chitosan (CS-coated Fe3O4 NPs) in one step by the solvothermal method in the presence of different amounts of added chitosan is reported here. The magnetic property of the obtained magnetic composite nanoparticles was confirmed
Dena L Cologgi et al.
Applied and environmental microbiology, 80(21), 6638-6646 (2014-08-17)
Biofilms formed by dissimilatory metal reducers are of interest to develop permeable biobarriers for the immobilization of soluble contaminants such as uranium. Here we show that biofilms of the model uranium-reducing bacterium Geobacter sulfurreducens immobilized substantially more U(VI) than planktonic
Christian Schach et al.
American journal of physiology. Heart and circulatory physiology, 307(8), H1093-H1102 (2014-08-17)
Impaired endothelial function, which is dysregulated in diabetes, also precedes hypertension. We hypothesized that in Type 2 diabetes, the impaired endothelium-dependent relaxation is due to a loss of endothelium-derived hyperpolarization (EDH) that is regulated by impaired ion channel function. Zucker
Cuiping Li et al.
Journal of chromatography. A, 1406, 324-330 (2015-06-25)
Oxidatively generated DNA damage is considered to be a significant contributing factor to cancer, aging, and age-related human diseases. It is important to detect oxidatively generated DNA damage to understand and clinically diagnosis diseases caused by oxidative damage. In this
Ying Qi et al.
PloS one, 10(7), e0132503-e0132503 (2015-07-15)
The absence of a productive cell culture system hampered detailed analysis of the structure and protein composition of the hepatitis E virion. In this study, hepatitis E virus from a robust HEV cell culture system and from the feces of
Matthew C Cook et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 997, 162-178 (2015-06-27)
The N-linked glycosylation of four lots of a marketed human therapeutic monoclonal antibody (mAb) was assessed by three orthogonal chromatographic methods and a commercial lectin microarray. For chromatography, the N-glycans were removed enzymatically from the mAbs using PNGase F. Native
David Awotwe-Otoo et al.
International journal of pharmaceutics, 490(1-2), 341-350 (2015-04-04)
The objective of the study was to analyze the effect of controlled and uncontrolled freezing step of a lyophilization process on the extent of non-enzymatic glycation and aggregation of an IgG1 formulation at two concentrations (1mg/ml and 20mg/ml). The degree
Luís M Magalhães et al.
Talanta, 147, 460-467 (2015-11-26)
Spent coffee grounds (SCGs) are a great source of bioactive compounds with interest to pharmaceutical and cosmetic industries. Phenolics and methylxanthines are the main health related compounds present in SCG samples. Content estimation of these compounds in SCGs is of
Shu-Wen Xue et al.
Journal of chromatography. A, 1411, 9-16 (2015-08-12)
Magnetic nanoparticles (MNPs) featured with divinylbenzene (DVB) and sulfonate functionalities (Fe3O4-DVB-SO3(-)) were prepared via "thiol-ene" click chemistry. The hydrophobic DVB moieties were dedicated for extraction while the hydrophilic sulfonate groups were designed for dispersing the MNPs in aqueous sample solution.
Mikaela A Lui et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 308(9), R779-R791 (2015-02-20)
The hypoxic and cold environment at high altitudes requires that small mammals sustain high rates of O2 transport for exercise and thermogenesis while facing a diminished O2 availability. We used laboratory-born and -raised deer mice (Peromyscus maniculatus) from highland and
Elina Esmaeilzadeh Gharehdaghi et al.
Nucleic acid therapeutics, 24(6), 420-427 (2014-10-02)
Chitosan nanoparticles were prepared using ultrasonication methodology at specific amplitudes and times of sonication. Subsequently, small interfering RNA (siRNA) was added to the solution at predetermined values of nitrogen to phosphorous ratio (N/P), and stirring time. Employing response surfaces generated
Wei Jia et al.
Journal of chromatography. A, 1362, 110-118 (2014-08-27)
A new analytical method was developed and validated for simultaneous analysis of 27 phthalates in milk and milk products. Response surface methodology was employed to optimize a quick, easy, cheap, effective, rugged, and safe (QuEChERS) sample preparation method. Ultrahigh-performance liquid
Wen-Hsing Chen et al.
Bioprocess and biosystems engineering, 37(7), 1337-1344 (2013-12-19)
This study evaluated a cost-effective approach for the conversion of rice straw into fermentable sugars. The composition of rice straw pretreated with 1 % sulfuric acid or 1 % sodium hydroxide solution was compared to rice straw with no chemical
Noah D Cohen et al.
PloS one, 9(10), e109865-e109865 (2014-10-22)
Neutrophils play an important role in protecting against infection. Foals have age-dependent deficiencies in neutrophil function that may contribute to their predisposition to infection. Thus, we investigated the ability of a CpG-ODN formulated with Emulsigen to modulate functional responses of
Akshaya Kumar Swain et al.
ACS applied materials & interfaces, 7(15), 8013-8022 (2015-03-31)
In light of the growing interest in the search for cheap and effective solutions for cancer treatment, we report a simple one pot synthesis of polymer stabilized iron oxide-graphene (PIG) that could be realized on a large scale. The structural
Justyna Sulej et al.
Applied biochemistry and biotechnology, 176(6), 1638-1658 (2015-05-25)
Cellobiose dehydrogenase (CDH), a secreted flavocytochrome produced by a number of wood-degrading fungi, was detected in the culture supernatant of a biotechnologically important strain of Cerrena unicolor grown in a modified cellulose-based liquid medium. The enzyme was purified as two
Karina Lopes Dornelas et al.
Analytica chimica acta, 858, 82-90 (2015-01-20)
This paper presents a simple and low-cost method for patterning poly(dimethylsiloxane) (PDMS) barriers in porous support such as paper for the construction of flexible microfluidic paper-based analytical devices (μPADs). The fabrication method consisted of contact-printing a solution of PDMS and
Daniel Girma Mulat et al.
Talanta, 143, 56-63 (2015-06-17)
In anaerobic digestion of organic matter, several metabolic pathways are involved during the simultaneous production and consumption of short-chain fatty acids (SCFA) in general and acetate in particular. Understanding the role of each pathway requires both the determination of the
Florian Platten et al.
The journal of physical chemistry. B, 119(48), 14986-14993 (2015-11-07)
Protein phase behavior and protein-protein interactions can be tuned by additives. We experimentally determined the phase behavior of lysozyme solutions, namely, the cloud-point temperature (CPT), in the presence of two additives, sodium chloride (NaCl) and guanidine hydrochloride (GuHCl). Their concentrations
Huili Wang et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 985, 62-70 (2015-02-11)
Herein, we developed a novel integrated device to perform phase separation based on ultrasound-assisted salt-induced liquid-liquid microextraction for determination of five fluoroquinones (FQs) in human body fluids. The integrated device consisted of three simple HDPE components used to separate the
Adriano Zager et al.
Brain, behavior, and immunity, 43, 159-171 (2014-08-12)
Multiple sclerosis (MS) is characterized by an autoimmune response against myelin antigens driven by autoreactive T cells. Several lines of evidence indicate that environmental factors, such as previous infection, can influence and trigger autoimmune responses. However, the importance of the
Tarin Nimmanwudipong et al.
Rapid communications in mass spectrometry : RCM, 29(24), 2337-2340 (2015-11-14)
Headspace solid-phase microextraction (HS-SPME) combined with gas chromatography/pyrolysis-gas chromatography/combustion-isotope ratio mass spectrometry (GC/Py-GC/C-IRMS) was developed for the simultaneous determination of the intramolecular and molecular carbon-isotopic composition (δ(13) C value) of acetic acid. The δ(13) C values of carboxyl and methyl
Hailong Yu et al.
Bioresource technology, 187, 161-166 (2015-04-08)
Green liquor (GL) combined with H2O2 (GL-H2O2) and green liquor (GL) combined with ethanol (GL-ethanol) were chosen for treating sugarcane bagasse. Results showed that the glucose yield (calculated from the glucose content as a percentage of the theoretical glucose available
Genlai Li et al.
The Journal of nutrition, 144(12), 1887-1895 (2014-10-17)
Short-chain fatty acids (SCFAs), including acetate, propionate, and butyrate, are the main products of microbial fermentation in the gut and might mediate some of the effects of gut microbiota and nutrition on development, metabolism, and pathogenesis of obesity and other
Yurong Liu et al.
Tissue engineering. Part A, 20(21-22), 3050-3062 (2014-05-03)
A therapy for regenerating large cartilaginous lesions within the articular surface of osteoarthritic joints remains elusive. While tissue engineering strategies such as matrix-assisted autologous chondrocyte implantation can be used in the repair of focal cartilage defects, extending such approaches to
Jung-Hoon Bae et al.
Scientific reports, 5, 12229-12229 (2015-07-22)
To produce rarely secreted recombinant proteins in the yeast Saccharomyces cerevisiae, we developed a novel genome-wide optimal translational fusion partner (TFP) screening system that involves recruitment of an optimal secretion signal and fusion partner. A TFP library was constructed from
Robert Glaser
Journal of biotechnology, 214, 9-16 (2015-09-02)
The characterization of cellulase performance for industrial-scale processes holds special challenges. A huge gap exists between the enzyme characterization in a laboratory and large-scale process performances. Common cellulase mixtures from Novozymes and enzymes provided by Moscow State University were used
Andrew D Shaw et al.
Annals of surgery, 255(5), 821-829 (2012-04-04)
To assess the association of 0.9% saline use versus a calcium-free physiologically balanced crystalloid solution with major morbidity and clinical resource use after abdominal surgery. 0.9% saline, which results in a hyperchloremic acidosis after infusion, is frequently used to replace
Lars Tatenhorst et al.
Journal of neuropathology and experimental neurology, 73(8), 770-779 (2014-07-09)
Chronic degeneration of nigrostriatal projections, followed by nigral dopaminergic cell death, is a key feature of Parkinson disease (PD). This study examines the neuroprotective potential of the rho kinase inhibitor fasudil in the 6-hydroxydopamine (6-OHDA) mouse model of PD in
Qingqing Wang et al.
Steroids, 96, 140-152 (2015-02-01)
An ultrasensitive stable isotope dilution liquid chromatography-tandem mass spectrometry method (LC-MS/MS) was developed and validated for multiplexed quantitative analysis of six unconjugated and conjugated estrogens in human serum. The quantification utilized a new derivatization procedure, which formed analytes as pre-ionized
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