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Monika Witala et al.
Soft matter, 11(29), 5883-5888 (2015-06-25)
Solid surfaces suspended in critical aqueous binary mixtures containing hydrophilic salt have recently been found to exhibit anomalous interactions, and a possible mechanism is provided by the asymmetric solvation preferences of weakly and strongly hydrophilic cations and anions, respectively. Here...
Zhenchang Liang et al.
PloS one, 9(8), e105146-e105146 (2014-08-19)
Grapes are rich in phytochemicals with many proven health benefits. Phenolic profiles, antioxidant and antiproliferative activities of twenty-four selected Vitis vinifera grape cultivars were investigated in this study. Large ranges of variation were found in these cultivars for the contents...
Maiko Kunii et al.
FEMS microbiology letters, 356(1), 45-52 (2014-05-28)
Osmoadaptation may be an important trait for the pathogenicity of Streptococcus mutans. However, how this organism adapts to changes in osmolality in the oral cavity remains unclear. In this study, we showed that S. mutans utilizes K(+) for osmoadaptation, in...
P A Léon Bouyou et al.
Analytical and bioanalytical chemistry, 406(20), 5019-5030 (2014-06-11)
Trace levels of the veterinary antibiotic compound sulfadiazine (SDZ) can be determined in agricultural drainage water samples with this new method. Optimized sample pretreatment and solid-phase extraction was combined with liquid chromatography coupled to tandem mass spectrometry (SPE LC-MS/MS) using...
Ping-Jung Su et al.
Scientific reports, 5, 13591-13591 (2015-09-01)
In vitro culture of dissociated retinal neurons is an important model for investigating retinal synaptic regeneration (RSR) and exploring potentials in artificial retina. Here, retinal precursor cells were cultured in a microfluidic chip with multiple arrays of microchannels in order...
Mika A Tamski et al.
Physical chemistry chemical physics : PCCP, 17(36), 23438-23447 (2015-08-21)
A miniaturised electrochemical cell design for Electron Paramagnetic Resonance (EPR) studies is reported. The cell incorporates a Loop Gap Resonator (LGR) for EPR investigation of electrochemically generated radicals in aqueous (and other large dielectric loss) samples and achieves accurate potential...
Christoph Haller et al.
Nucleic acid therapeutics, 25(1), 20-26 (2014-12-11)
A multitude of stem cell types has been extensively studied and used for myocardial regenerative therapy. Amongst these endothelial progenitor cells form a promising source. In our present study, we investigated a one-staged approach for isolation and application of autologous...
D P Froman
Poultry science, 92(5), 1336-1342 (2013-04-11)
Previous research demonstrated that sperm motility is dependent upon mitochondrial calcium cycling. Thus, sperm are inactivated when extracellular calcium ions are chelated. Mitochondrial calcium cycling, however, is driven by extracellular sodium ions. The hypothesis that sperm inactivation is subject to...
Blair K Gage et al.
Islets, 6(2), e29236-e29236 (2014-12-09)
Human embryonic stem cells (hESCs) are pluripotent and capable of generating new β-cells, but current in vitro differentiation protocols generally fail to produce mature, glucose-responsive, unihormonal β-cells. Instead, these methods tend to produce immature polyhormonal endocrine cells which mature in...
Paolo Massobrio et al.
BMC neuroscience, 14, 22-22 (2013-02-28)
A large number of invertebrate models, including the snail Helix, emerged as particularly suitable tools for investigating the formation of synapses and the specificity of neuronal connectivity. Helix neurons can be individually identified and isolated in cell culture, showing well-conserved...
S C Perry et al.
Physical chemistry chemical physics : PCCP, 17(44), 30005-30012 (2015-10-27)
Using chronoamperometry at preconditioned oxide-free Pt microdisc electrodes in aqueous media, we investigated the oxygen reduction reaction (ORR) on the millisecond timescale and obtained results consistent with the reduction of oxygen species which adsorb on the electrode before the ORR...
Francisco E Martín-Cano et al.
American journal of physiology. Gastrointestinal and liver physiology, 306(7), G614-G621 (2014-02-15)
The main roles of the colonic mucosa are the absorption of water and electrolytes and the barrier function that preserves the integrity of the colonic wall. The mediators and mechanisms to accomplish these functions are under continuous investigation, but little...
Evelien Wynendaele et al.
PloS one, 10(11), e0142071-e0142071 (2015-11-05)
Bacteria communicate with each other by the use of signaling molecules, a process called 'quorum sensing'. One group of quorum sensing molecules includes the oligopeptides, which are mainly produced by Gram-positive bacteria. Recently, these quorum sensing peptides were found to...
Frida Loría et al.
Neurobiology of disease, 76, 1-12 (2015-01-03)
Friedreich's ataxia (FA) is a recessive, predominantly neurodegenerative disorder caused in most cases by mutations in the first intron of the frataxin (FXN) gene. This mutation drives the expansion of a homozygous GAA repeat that results in decreased levels of...
Petra Školáková et al.
Nucleic acids research, 43(9), 4733-4745 (2015-04-10)
There are two basic mechanisms that are associated with the maintenance of the telomere length, which endows cancer cells with unlimited proliferative potential. One mechanism, referred to as alternative lengthening of telomeres (ALT), accounts for approximately 10-15% of all human...
High-resolution in situ hybridization to whole-mount zebrafish embryos.
Thisse C and Thisse B
Nature Protocols, 3(1), 59-69 (2008)
Charles J McDonald et al.
Neurochemistry international, 88, 10-14 (2014-12-17)
The GLS1 gene encodes a mitochondrial glutaminase that is highly expressed in brain, kidney, small intestine and many transformed cells. Recent studies have identified multiple lysine residues in glutaminase that are sites of N-acetylation. Interestingly, these sites are located within...
Tzu-Wei Lin et al.
Current Alzheimer research, 12(10), 951-963 (2015-10-28)
Both the hippocampus and amygdala are early vulnerable brain regions in the development of Alzheimer's disease (AD). However, previous studies mainly focused on characterizing the hippocampus in the pathophysiology of AD, leaving the amygdala less explored. Here, we characterized the...
L-B Li et al.
Transplantation proceedings, 45(2), 523-527 (2013-03-19)
Clinically, donor hearts cannot be preserved for >6 hours between explantation and recipient implantation. A better approach is needed to preserve donor hearts for a longer time. We tested whether low-flow perfusion (LFP) could satisfactorily preserve isolated rat hearts with...
Yuriko Furukawa et al.
Biochimica et biophysica acta, 1830(9), 4329-4333 (2013-03-05)
Transplantation is one potential clinical application of neural stem cells (NSCs). However, it is very difficult to monitor/control NSCs after transplantation and so provide effective treatment. Electrical measurement using a poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) (PEDOT-PSS) modified microelectrode array (MEA) is a biocompatible, non-invasive...
C Beinke et al.
Radiation research, 184(1), 66-72 (2015-07-08)
The accidental gamma radiation exposure of an industrial radiography worker and the cytogenetic examination of the worker's blood lymphocytes are described here. The exposure of the worker was due to a malfunction at the entrance into the depleted uranium-shielding device...
Alexandra Malachová et al.
Analytical and bioanalytical chemistry, 407(20), 6009-6020 (2015-06-13)
A critical assessment of three previously published indirect methods based on acidic hydrolysis using superacids for the determination of "free" and "total" deoxynivalenol (DON) was carried out. The modified mycotoxins DON-3-glucoside (D3G), 3-acetyl-DON (3ADON), and 15-acetyl-DON (15ADON) were chosen as...
J Marcus et al.
Physical chemistry chemical physics : PCCP, 17(48), 32528-32538 (2015-11-26)
We study the addition of electrolytes to surfactant-free microemulsions in the domain where polydisperse pre-Ouzo aggregates are present. As in previous studies, the microemulsion is the ternary system water/ethanol/1-octanol, where ethanol acts as co-solvent. Addition of electrolytes modifies the static...
Saeyoung Park et al.
Cytotechnology, 67(5), 851-860 (2014-07-07)
Although stem cells are promising candidates for cell replacement therapies, the vast majority are derived using animal sera, which has risk of being contaminated by animal viruses or toxins. To overcome these potential problems, we initially established multiple lines of...
Claudio Hidalgo-Cantabrana et al.
Applied and environmental microbiology, 81(23), 7960-7968 (2015-09-13)
Exopolysaccharides (EPS) are extracellular carbohydrate polymers synthesized by a large variety of bacteria. Their physiological functions have been extensively studied, but many of their roles have not yet been elucidated. We have sequenced the genomes of two isogenic strains of...
Merve Bacanlı et al.
Environmental toxicology and pharmacology, 38(3), 774-782 (2014-10-13)
Oxidative stress has an important role in the development of sepsis-induced multiorgan failure. Ferulic acid (FA), a well-established natural antioxidant, has several pharmacological activities including anti-inflammatory, anticancer and hepatoprotective. This study aimed to investigate the effects of FA on sepsis-induced...
Edgar Santos et al.
Acta neurochirurgica. Supplement, 118, 93-97 (2013-07-24)
The aim of this study was to co-record electrical changes using electrocorticography (ECoG) and blood volume changes using intrinsic optical signal (IOS) imaging during the induction, propagation, and termination of cortical spreading depolarizations (CSDs). Anesthetized male swine were craniotomized and...
Miriam Reisenhofer et al.
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie, 253(5), 721-731 (2015-04-16)
To identify programmed cell death (PCD) pathways involved in N-methyl-N-nitrosourea (MNU)-induced photoreceptor (PR) degeneration. Adult C57BL/6 mice received a single MNU i.p. injection (60 mg/kg bodyweight), and were observed over a period of 7 days. Degeneration was visualized by H&E...
J Souframanien et al.
PloS one, 10(6), e0128748-e0128748 (2015-06-05)
Black gram [V. mungo (L.) Hepper] is an important legume crop extensively grown in south and south-east Asia, where it is a major source of dietary protein for its predominantly vegetarian population. However, lack of genomic information and markers has...
Maria E Grimmett
Journal of environmental quality, 44(4), 1183-1192 (2015-10-06)
Four hundred tons of sulfamethazine are fed to livestock annually in North America for disease prevention and growth promotion, but the majority is excreted unmetabolized into the environment. Due to its slow degradation and high mobility, sulfamethazine contaminates groundwater and...
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