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Verena K Hehle et al.
BMC biotechnology, 11, 128-128 (2012-01-03)
Interest in using plants for production of recombinant proteins such as monoclonal antibodies is growing, but proteolytic degradation, leading to a loss of functionality and complications in downstream purification, is still a serious problem. In this study, we investigated the
Tegwen Marlais et al.
PloS one, 15(9), e0238840-e0238840 (2020-09-15)
Diagnosis of visceral leishmaniasis (VL) relies on invasive and risky aspirate procedures, and confirmation of cure after treatment is unreliable. Detection of Leishmania donovani antigens in urine has the potential to provide both a non-invasive diagnostic and a test of
Boris Gorbatyuk et al.
Molecular microbiology, 55(4), 1233-1245 (2005-02-03)
DnaA protein binds bacterial replication origins and it initiates chromosome replication. The Caulobacter crescentus DnaA also initiates chromosome replication and the C. crescentus response regulator CtrA represses chromosome replication. CtrA proteolysis by ClpXP helps restrict chromosome replication to the dividing
Lu Chen et al.
Cell, 174(1), 218-230 (2018-05-29)
Ribonucleoprotein enzymes require dynamic conformations of their RNA constituents for regulated catalysis. Human telomerase employs a non-coding RNA (hTR) with a bipartite arrangement of domains-a template-containing core and a distal three-way junction (CR4/5) that stimulates catalysis through unknown means. Here
Satya Dash et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(23), 9350-9355 (2009-05-28)
Tre-2, BUB2, CDC16, 1 domain family member 4 (TBC1D4) (AS160) is a Rab-GTPase activating protein implicated in insulin-stimulated glucose transporter 4 (GLUT4) translocation in adipocytes and myotubes. To determine whether loss-of-function mutations in TBC1D4 might impair GLUT4 translocation and cause
Aroa Ejarque-Ortiz et al.
Journal of neuroscience research, 88(5), 1113-1123 (2009-11-13)
The transcription factor CCAAT/enhancer binding protein delta (C/EBP delta) regulates transcription of genes that play important roles in glial activation. Previous studies have shown the astroglial expression of C/EBP delta but the microglial expression of C/EBP delta remains virtually unexplored
Andreas O Doesch et al.
Journal of clinical apheresis, 25(6), 315-322 (2010-09-09)
The objective of this study was to investigate functional effects of immunoadsorption (IA) in patients with chronic nonfamilial dilated cardiomyopathy (DCM) regarding clinical and humoral markers of heart failure. IA has been shown to induce early hemodynamic improvement in patients
Yu Ya Kit et al.
Biochemistry. Biokhimiia, 73(8), 950-956 (2008-09-09)
Immunoglobulins IgG and sIgA actively hydrolyzing histone H1 have been detected on analyzing proteolytic activity of antibodies isolated by chromatography on Protein A-agarose from blood serum of patients with multiple sclerosis and from colostrum of healthy mothers. These antibodies hydrolyze
Hans H Bock et al.
The Journal of biological chemistry, 278(40), 38772-38779 (2003-07-29)
Reelin is a large secreted signaling protein that binds to two members of the low density lipoprotein receptor family, the apolipoprotein E receptor 2 and the very low density lipoprotein receptor, and regulates neuronal positioning during brain development. Reelin signaling
Hsin-Yi Henry Ho et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(11), 4044-4051 (2012-02-22)
Wnts make up a large family of extracellular signaling molecules that play crucial roles in development and disease. A subset of noncanonical Wnts signal independently of the transcription factor β-catenin by a mechanism that regulates key morphogenetic movements during embryogenesis.
Phosphoprotein Inhibitors of Protein Phosphatase-1
Eto, M., et al.
Methods in Enzymology, 366, 241-260 (2003)
Nsi1 plays a significant role in the silencing of ribosomal DNA in <I>Saccharomyces cerevisiae</I>.
Ha, C.W., et al.
Nucleic Acids Research (2012)
Stable Isotope Labeling with Amino Acids in Cell Culture (SILAC) for Studying Dynamics of Protein Abundance and Posttranslational Modifications.
Amanchy, R., et al.
Science Signaling, 2005(267), pI2-pI2 (2005)
Y Shimazaki et al.
Journal of biochemical and biophysical methods, 37(1-2), 1-4 (1998-11-24)
Protein spots, such as immunoglobulin G (IgG), A (IgA) or alpha 2-macroglobulin, on the patterns of non-denaturing two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) were selectively removed by the application of protein A or antibody-conjugated protein A agarose to the first-dimension
Masumi Eto et al.
The Journal of biological chemistry, 282(24), 17806-17815 (2007-04-17)
Focal adhesions attach cultured cells to the extracellular matrix, and we found endogenous protein phosphatase-1alpha isoform (PP1alpha) localized in adhesions across the entire area of adherent fibroblasts. However, in fibroblasts migrating into a scrape wound or spreading after replating PP1alpha
Tingting Guo et al.
International journal of molecular sciences, 20(10) (2019-05-28)
Leaf blade width, curvature, and cuticular wax are important agronomic traits of rice. Here, we report the rice Oschr4-5 mutant characterized by pleiotropic phenotypes, including narrow and rolled leaves, enhanced cuticular wax deposition and reduced plant height and tiller number.
Lu Chen et al.
Cell reports, 30(5), 1358-1372 (2020-02-06)
Biogenesis of the human telomerase RNA (hTR) involves a complex series of posttranscriptional modifications, including hypermethylation of the 5' mono-methylguanosine cap to a tri-methylguanosine cap (TMG). How the TMG cap affects hTR maturation is unknown. Here, we show that depletion
Hans H Bock et al.
The Journal of biological chemistry, 279(32), 33471-33479 (2004-06-04)
The cytoplasmic adaptor protein Disabled-1 (Dab1) is necessary for the regulation of neuronal positioning in the developing brain by the secreted molecule Reelin. Binding of Reelin to the neuronal apolipoprotein E receptors apoER2 and very low density lipoprotein receptor induces
K Klaamas et al.
Neoplasma, 55(2), 143-150 (2008-02-02)
All human immunoglobulins are glycosylated. The changes in IgG glycosylation are associated with autoimmune disorders and pregnancy. Little is known about IgG glycosylation in patients with cancer. A lectin enzyme-linked immunosorbent assay (LELISA) based method was developed for measuring the
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