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C Widmann et al.
The Journal of biological chemistry, 273(12), 7141-7147 (1998-04-18)
Caspases are activated during apoptosis and cleave specific proteins, resulting in the irreversible commitment to cell death. The signal transduction proteins MEKK1, p21-activated kinase 2, and focal adhesion kinase are caspase substrates that contribute to the cell death response when
Thomas A Hall et al.
RNA (New York, N.Y.), 8(3), 296-306 (2002-05-11)
Although archaeal RNase P RNAs are similar in both sequence and structure to those of Bacteria rather than eukaryotes, and heterologous reconstitution between the Bacillus subtilis RNase P protein and some archaeal RNase P RNAs has been demonstrated, no archaeal
Tracie A Hennen-Bierwagen et al.
Plant physiology, 149(3), 1541-1559 (2009-01-27)
Starch biosynthetic enzymes from maize (Zea mays) and wheat (Triticum aestivum) amyloplasts exist in cell extracts in high molecular weight complexes; however, the nature of those assemblies remains to be defined. This study tested the interdependence of the maize enzymes
T H Sisson et al.
Journal of immunological methods, 127(2), 215-220 (1990-03-09)
This report describes a modification of a procedure developed by others for crosslinking IgG to protein A which itself is covalently linked to a gel support. Earlier immunoaffinity columns were described as having large antigen-binding capacities and stability under a
Junfei Gao et al.
Biomaterials, 33(11), 3344-3352 (2012-01-25)
Co-immunoprecipitation is a very effective method for studying protein-protein interactions. However, the preparation of antibodies in this method involves the injection of antigen into mammals, and requires the use of the expensive protein A-Sepharose 4B. Molecular imprinting polymer can compensate
Felicity Johnson et al.
PloS one, 4(9), e7100-e7100 (2009-09-19)
The mitochondrial protease OMI (also known as HtrA2) has been implicated in Parkinson's Disease (PD) and deletion or protease domain point mutations have shown profound neuropathologies in mice. A beneficial role by OMI, in preserving cell viability, is assumed to
Theophany Eystathioy et al.
Molecular biology of the cell, 13(4), 1338-1351 (2002-04-16)
A novel human cellular structure has been identified that contains a unique autoimmune antigen and multiple messenger RNAs. This complex was discovered using an autoimmune serum from a patient with motor and sensory neuropathy and contains a protein of 182
Dharmendra Pandey et al.
Blood, 107(2), 575-583 (2005-10-13)
Cofilin is a regulator of actin filament dynamics. We studied whether during platelet activation Rho kinase stimulates LIM kinase (LIMK) leading to subsequent phosphorylation and inactivation of cofilin. Platelet shape change and aggregation/secretion were induced by low and high concentrations
Fariba Vaziri-Sani et al.
Journal of immunological methods, 371(1-2), 25-37 (2011-06-29)
Autoantibodies against the zinc transporter 8 (ZnT8A) are common in type 1 diabetes (T1D). ZnT8A analyses are complicated by the fact that there are three variants of the autoantigen at amino acid position 325 representing ZnT8-R (Arginine), ZnT8-W (Tryptophan) and
Olga Khorosjutina et al.
The Journal of biological chemistry, 285(39), 29729-29737 (2010-07-14)
The multiprotein Mediator complex is an important regulator of RNA polymerase II-dependent genes in eukaryotic cells. In contrast to the situation in many other eukaryotes, the conserved Med15 protein is not a stable component of Mediator isolated from fission yeast.
Jyoti Parkash et al.
Endocrinology, 151(6), 2723-2735 (2010-04-08)
Within the preoptic region, nitric oxide (NO) production varies during the ovarian cycle and has the ability to impact hypothalamic reproductive function. One mechanism for the regulation of NO release mediated by estrogens during the estrous cycle includes physical association
Wassamon Boonying et al.
Journal of neurochemistry, 150(3), 312-329 (2019-02-09)
Loss of function mutations in the PTEN-induced putative kinase 1 (Pink1) gene have been linked with an autosomal recessive familial form of early onset Parkinson's disease (PD). However, the underlying mechanism(s) responsible for degeneration remains elusive. Presently, using co-immunoprecipitation in
Pramila Rijal et al.
Cell reports, 27(1), 172-186 (2019-04-04)
We describe therapeutic monoclonal antibodies isolated from human volunteers vaccinated with recombinant adenovirus expressing Ebola virus glycoprotein (EBOV GP) and boosted with modified vaccinia virus Ankara. Among 82 antibodies isolated from peripheral blood B cells, almost half neutralized GP pseudotyped
S H Jung et al.
Cell death and differentiation, 23(3), 417-429 (2015-08-08)
Cellular senescence is considered as an important tumor-suppressive mechanism. Here, we demonstrated that heparan sulfate (HS) prevents cellular senescence by fine-tuning of the fibroblast growth factor receptor (FGFR) signaling pathway. We found that depletion of 3'-phosphoadenosine 5'-phosphosulfate synthetase 2 (PAPSS2)
Eri Miyagi et al.
Journal of virology, 94(7) (2020-01-17)
Vif counteracts the host restriction factor APOBEC3G (A3G) and other APOBEC3s by preventing the incorporation of A3G into progeny virions. We previously identified Vif mutants with a dominant-negative (D/N) phenotype that interfered with the function of wild-type Vif, inhibited the
J W Zhang et al.
The Journal of cell biology, 132(3), 325-334 (1996-02-01)
Peb1 is a peroxisome biogenesis mutant isolated in Saccharomyces cerevisiae that is selectively defective in the import of thiolase into peroxisomes but has a normal ability to package catalase, luciferase and acyl-CoA oxidase (Zhang, J. W., C. Luckey, and P.
Frédéric Sorgeloos et al.
Journal of virology, 85(7), 3690-3694 (2011-01-14)
The L protein encoded by Theiler's murine encephalomyelitis virus (TMEV) is a unique example of a picornaviral protein encoded by an alternative open reading frame. This protein is an important determinant of TMEV persistence in the mouse central nervous system.
P Hornbeck et al.
Molecular and cellular biology, 9(9), 3727-3735 (1989-09-01)
The regulation and expression of protein kinase C (PKC) and phosphomyristin C (PMC) (a principal substrate of PKC which is the major myristylated protein in lymphocyte and glioma lines that express it) in murine B and T lymphocytes were investigated.
M Iezzi et al.
Molecular endocrinology (Baltimore, Md.), 13(2), 202-212 (1999-02-11)
Insulin-secreting cells express four GTPases of the Rab3 family. After separation of extracts of INS-1 cells on a sucrose density gradient, the bulk of the A, B, and C isoforms was recovered in the fractions enriched in insulin-containing secretory granules.
A Kishkevich et al.
Scientific reports, 9(1), 10686-10686 (2019-07-25)
Activation of cell cycle regulated transcription during the G1-to-S transition initiates S phase entry and cell cycle commitment. The molecular mechanisms involving G1/S transcriptional regulation are well established and have been shown to be evolutionary conserved from yeast to humans.
Bjorn-Patrick Mohl et al.
Journal of virology, 93(20) (2019-08-04)
The molecular chaperone machinery is important for the maintenance of protein homeostasis within the cells. The principle activities of the chaperone machinery are to facilitate protein folding and organize conformationally dynamic client proteins. Prominent among the members of the chaperone
Sunandini Chandra et al.
Molecular biology of the cell, 28(26), 3801-3814 (2017-10-28)
Reconstituted Sendai viral envelopes (virosomes) are well recognized for their promising potential in membrane fusion-mediated delivery of bioactive molecules to liver cells. Despite the known function of viral envelope glycoproteins in catalyzing fusion with cellular membrane, the role of host
Kristina Drizyte-Miller et al.
Journal of cell science, 133(11) (2020-04-17)
Epithelial cells, such as liver-resident hepatocytes, rely heavily on the Rab family of small GTPases to perform membrane trafficking events that dictate cell physiology and metabolism. Not surprisingly, disruption of several Rab proteins can manifest in metabolic diseases or cancer.
Aiqin Sun et al.
Autophagy, 13(3), 522-537 (2017-01-14)
The MAP1LC3/LC3 family plays an essential role in autophagosomal biogenesis and transport. In this report, we show that the HECT family E3 ubiquitin ligase NEDD4 interacts with LC3 and is involved in autophagosomal biogenesis. NEDD4 binds to LC3 through a
Shay Sela et al.
Circulation research, 102(12), 1566-1574 (2008-06-03)
A human-specific splicing variant of vascular endothelial growth factor (VEGF) receptor 1 (Flt1) was discovered, producing a soluble receptor (designated sFlt1-14) that is qualitatively different from the previously described soluble receptor (sFlt1) and functioning as a potent VEGF inhibitor. sFlt1-14
J H Ellis et al.
Journal of immunology (Baltimore, Md. : 1950), 164(11), 5805-5814 (2000-05-23)
Adapter proteins such as Grb2 play a central role in the formation of signaling complexes through their association with multiple protein binding partners. These interactions are mediated by specialized domains such as the well-characterized Src homology SH2 and SH3 motifs.
Johannes F Van Agthoven et al.
Structure (London, England : 1993), 27(9), 1443-1451 (2019-07-30)
Targeting both integrins αVβ3 and α5β1 simultaneously appears to be more effective in cancer therapy than targeting each one alone. The structural requirements for bispecific binding of ligand to integrins have not been fully elucidated. RGD-containing knottin 2.5F binds selectively
Grace O Iyirhiaro et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 37(28), 6729-6740 (2017-06-14)
Dysregulation of cell cycle machinery is implicated in a number of neuronal death contexts, including stroke. Increasing evidence suggests that cyclin-dependent kinases (Cdks) are inappropriately activated in mature neurons under ischemic stress conditions. We previously demonstrated a functional role for
Jennifer Kjellerås et al.
Scandinavian journal of clinical and laboratory investigation, 71(8), 701-704 (2011-10-04)
Tissue transglutaminase (tTG) autoantibodies are serological markers for celiac disease. The aim was to study the efficacy of the pTnT-rhtTG plasmid and subsequent diagnostic accuracy of tTG autoantibodies for childhood celiac disease using radioligand binding assays. Coupled in vitro transcription
M J Coffey et al.
The Journal of pharmacology and experimental therapeutics, 299(1), 198-203 (2001-09-19)
We have previously shown that the ability of overnight pretreatment with lipopolysaccharide (LPS) to suppress alveolar macrophage (AM) leukotrienes (LT) synthesis is explained by induction of nitric oxide (NO), and reactive oxygen intermediates (ROI). More recently we have demonstrated that
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