Showing 1-30 of 256 results for "

P1379

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Lili Lu et al.
Antioxidants & redox signaling, 11(4), 715-724 (2008-10-01)
Oxidative damage contributes to cone cell death in retinitis pigmentosa and death of rods, cones, and retinal pigmented epithelial (RPE) cells in age-related macular degeneration. In this study, we explored the strategy of overexpressing components of the endogenous antioxidant defense...
Marc J Tol et al.
Autophagy, 14(3), 437-449 (2018-02-20)
In recent years, the lysosome has emerged as a highly dynamic, transcriptionally regulated organelle that is integral to nutrient-sensing and metabolic rewiring. This is coordinated by a lysosome-to-nucleus signaling nexus in which MTORC1 controls the subcellular distribution of the microphthalmia-transcription...
Peter T Beernink et al.
Infection and immunity, 76(9), 4232-4240 (2008-07-02)
No broadly protective vaccine is available for the prevention of group B meningococcal disease. One promising candidate is factor H-binding protein (fHbp), which is present in all strains but often sparsely expressed. We prepared seven murine immunoglobulin G monoclonal antibodies...
Peter Pastuszko et al.
European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery, 35(4), 577-581 (2009-02-17)
To determine the effect of recovery with mild hypothermia after cardiopulmonary bypass (CPB) and deep hypothermic circulatory arrest (DHCA) on the activity of selected key proteins involved in initiation (Bax, Caspase-3) or inhibition of apoptotic injury (Bcl-2, increased ratio Bcl-2/Bax)...
Helena Zientek-Targosz et al.
Molecular cancer, 7, 51-51 (2008-06-07)
Widely accepted somatic mutation theory of carcinogenesis states that mutations in oncogenes and tumor suppressor genes in genomes of somatic cells is the cause of neoplastic transformation. Identifying frequent mutations in cancer cells suggests the involvement of mutant genes in...
Margherita Leonardi et al.
Autophagy, 15(2), 312-326 (2018-10-06)
Macroautophagy/autophagy is one of the major responses to stress in eukaryotic cells and is implicated in several pathological conditions such as infections, neurodegenerative diseases and cancer. Interestingly, cancer cells take full advantage of autophagy both to support tumor growth in...
Shmuel Negussie et al.
Journal of neurochemistry, 148(1), 46-62 (2018-10-23)
βarrestin (βarr)-1 and -2 are ubiquitously (outside the retina) expressed G-protein-coupled receptor adapter proteins. They uncouple G-protein-coupled receptors from G proteins, internalize the receptor, and subsequently initiate their own wave of signaling independently of G proteins. Angiotensin (Ang) II type...
Rubhana Raqib et al.
Human vaccines & immunotherapeutics, 15(6), 1326-1337 (2019-02-23)
Shigella sonnei live vaccine candidate, WRSS1, which was previously evaluated in US, Israeli and Thai volunteers, was administered orally to Bangladeshi adults and children to assess its safety, clinical tolerability and immunogenicity. In a randomized, placebo-controlled, dose-escalation, age-descending study, 39...
Tibor Sághy et al.
Cell death & disease, 10(6), 439-439 (2019-06-06)
Transglutaminase 2 (TG2) is a multifunctional protein that promotes clearance of apoptotic cells (efferocytosis) acting as integrin β3 coreceptor. Accumulating evidence indicates that defective efferocytosis contributes to the development of chronic inflammatory diseases. Obesity is characterized by the accumulation of...
Margaret O'Brien et al.
Reproductive biology and endocrinology : RB&E, 6, 1-1 (2008-01-15)
RHOGTPases play a significant role in modulating myometrial contractility in uterine smooth muscle. They are regulated by at least three families of proteins, RHO guanine nucleotide exchange factors (RHOGEFs), RHOGTPase-activating proteins (RHOGAPs) and RHO guanine nucleotide inhibitors (RHOGDIs). RHOGEFs activate...
Gamze Dereli Can et al.
Cytotherapy, 21(1), 83-95 (2018-12-05)
Several methods to cultivate limbal epithelial stem cells (LESCs) in vitro with the support of feeder layers and different growth medium formulations have been established for several years. The initial green medium consists of various ingredients that exhibit a non-optimal...
Riccardo Cristofani et al.
Autophagy, 13(8), 1280-1303 (2017-04-14)
Motoneuron diseases, like spinal bulbar muscular atrophy (SBMA) and amyotrophic lateral sclerosis (ALS), are associated with proteins that because of gene mutation or peculiar structures, acquire aberrant (misfolded) conformations toxic to cells. To prevent misfolded protein toxicity, cells activate a...
Natalia Felitsyn et al.
Journal of neurochemistry, 106(5), 2068-2079 (2008-07-31)
Delta-aminolevulinic acid (delta-ALA) is a heme precursor implicated in neurological complications associated with porphyria and tyrosinemia type I. Delta-ALA is also elevated in the urine of animals and patients treated with the investigational drug dichloroacetate (DCA). We postulated that delta-ALA...
Daniel C Zielinski et al.
Nature communications, 6, 7101-7101 (2015-06-10)
Drug side effects cause a significant clinical and economic burden. However, mechanisms of drug action underlying side effect pathogenesis remain largely unknown. Here, we integrate pharmacogenomic and clinical data with a human metabolic network and find that non-pharmacokinetic metabolic pathways...
Mads Skytte Rasmussen et al.
Autophagy, 13(5), 834-853 (2017-03-14)
The cysteine protease ATG4B cleaves off one or more C-terminal residues of the inactive proform of proteins of the ortholog and paralog LC3 and GABARAP subfamilies of yeast Atg8 to expose a C-terminal glycine that is conjugated to phosphatidylethanolamine during...
Gamze Dereli Can et al.
Experimental eye research, 173, 1-12 (2018-04-22)
Platelet-rich fibrin (PRF) is a natural biomaterial and has excellent biochemical and physical properties with a history of proven biocompatibility in the field of tissue engineering and regenerative medicine. Recent reports of fibrin-based matrices have offered new opportunities to apply...
Beatriz Alvarez-Castelao et al.
Nature protocols, 14(2), 556-575 (2019-01-06)
A big challenge in proteomics is the identification of cell-type-specific proteomes in vivo. This protocol describes how to label, purify and identify cell-type-specific proteomes in living mice. To make this possible, we created a Cre-recombinase-inducible mouse line expressing a mutant...
Tanya Fradin et al.
Journal of leukocyte biology, 104(5), 1023-1039 (2018-07-17)
A central event in the activation of the phagocyte NADPH oxidase involves binding of p67phox to the dehydrogenase region of Nox2. The identity of the binding site in Nox2 is unknown. By measuring binding of p67phox to synthetic Nox2 peptides...
Atsushi Kambe et al.
Cancer prevention research (Philadelphia, Pa.), 2(12), 1088-1099 (2009-11-26)
EP4 expression in human glioblastoma cells correlates with growth on soft agar. The cyclooxygenase inhibitor sulindac sulfide first altered specificity protein-1 (Sp-1) and early growth response gene-1 expression, then increased the expression of nonsteroidal anti-inflammatory drug-activated gene 1 and activating...
Ilda Theka et al.
Scientific reports, 9(1), 948-948 (2019-02-01)
Mouse embryonic stem cells (mESCs) are pluripotent and can differentiate into cells belonging to the three germ layers of the embryo. However, mESC pluripotency and genome stability can be compromised in prolonged in vitro culture conditions. Several factors control mESC...
Shahid Hussain et al.
Scientific reports, 8(1), 15785-15785 (2018-10-27)
Spontaneously occurring canine mammary tumours (CMTs) are the most common neoplasms of female unspayed dogs and are of potential importance as models for human breast cancer as well. Mortality rates are thrice higher in dogs as compared to humans with...
Paul Bourgine et al.
Stem cell research, 12(2), 584-598 (2014-02-25)
The hTERT-immortalization of human bone marrow-derived Mesenchymal Stromal Cells (hMSCs) was proposed to address availability/standardization issues for experimental or clinical studies, but raised concerns due to possible uncontrolled growth or malignant cell transformation. Here we report a method to generate...
Joseph G Dubouzet et al.
Plant biotechnology journal, 9(4), 466-485 (2010-10-20)
Approximately 20,000 of the rice-FOX Arabidopsis transgenic lines, which overexpress 13,000 rice full-length cDNAs at random in Arabidopsis, were screened for bacterial disease resistance by dip inoculation with Pseudomonas syringae pv. tomato DC3000 (Pst DC3000). The identities of the overexpressed...
Christopher D L Johnson et al.
ACS applied materials & interfaces, 11(1), 356-372 (2018-12-06)
Magnetic electrospun fibers are of interest for minimally invasive biomaterial applications that also strive to provide cell guidance. Magnetic electrospun fibers can be injected and then magnetically positioned in situ, and the aligned fiber scaffolds provide consistent topographical guidance to...
Martin Kongsbak et al.
PloS one, 9(5), e96695-e96695 (2014-05-06)
The active form of vitamin D3, 1,25(OH)2D3, has significant immunomodulatory properties and is an important determinant in the differentiation of CD4+ effector T cells. The biological actions of 1,25(OH)2D3 are mediated by the vitamin D receptor (VDR) and are believed...
Takayuki Nakayama et al.
International journal of hematology, 87(4), 363-370 (2008-03-29)
von Willebrand factor (VWF) performs its hemostatic functions through binding to various proteins. The A1 domain of VWF contains binding sites of not only physiologically important ligands, but also exogenous modulators that induce VWF-platelet aggregation. Sulfatides, 3-sulfated galactosyl ceramides, that...
Antonia Rotolo et al.
Cancer cell, 34(4), 596-610 (2018-10-10)
Chimeric antigen receptor anti-CD19 (CAR19)-T cell immunotherapy-induced clinical remissions in CD19+ B cell lymphomas are often short lived. We tested whether CAR19-engineering of the CD1d-restricted invariant natural killer T (iNKT) cells would result in enhanced anti-lymphoma activity. CAR19-iNKT cells co-operatively activated...
Guangyuan Li et al.
Molecular vision, 17, 3156-3165 (2011-12-16)
The receptor for advanced glycation end products (RAGE) has been implicated in the pathogenesis of numerous complications of diabetes. We assessed the effect of a novel RAGE fusion protein inhibitor on retinal histopathology and nerve function, and on retinal inflammation...
Kiran Kundu et al.
Cancer immunology research, 7(7), 1120-1134 (2019-06-06)
mAb-based blocking of the immune checkpoints involving the CTLA4-B7 and PD1-PDL1 inhibitory axes enhance T-cell-based adaptive immune responses in patients with cancer. We show here that antitumor responses by natural killer (NK) cells can be enhanced by a checkpoint-blocking mAb...
Kowit Yu Chong et al.
Cancer biology & therapy, 16(1), 149-159 (2015-01-16)
Multiple drug resistance (MDR) is a major obstacle to attenuating the effectiveness of chemotherapy to many human malignancies. Proteasome inhibition induces apoptosis in a variety of cancer cells and is recognized as a novel anticancer therapy approach. Despite its success...