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Fiona B McDonald et al.
Experimental physiology, 100(8), 947-966 (2015-06-23)
What is the central question of this study? Chronic intermittent hypoxia (CIH) is a dominant feature of respiratory control disorders, which are common. We sought to examine the effects of exposure to CIH during neonatal development on respiratory muscle form
R Hisada et al.
Journal of biochemistry, 82(5), 1469-1473 (1977-11-01)
This paper describes the properties and application of 1-methoxy-5-methylphenazinium methyl sulfate (1-methoxyPMS), which is a photochemically stable, versatile electron carrier. Like 5-methylphenazinium methyl sulfate (PMS), it mediates electron transfer between NADH and various electron acceptors such as tetrazolium dyes or
Yasuaki Anami et al.
Nature communications, 9(1), 2512-2512 (2018-06-30)
Valine-citrulline linkers are commonly used as enzymatically cleavable linkers for antibody-drug conjugates. While stable in human plasma, these linkers are unstable in mouse plasma due to susceptibility to an extracellular carboxylesterase. This instability often triggers premature release of drugs in
Early life exposure to chronic intermittent hypoxia causes upper airway dilator muscle weakness, which persists into young adulthood
McDonald FB, et al.
Experimental Physiology, 100(8), 947-966 (2015)
Debbie-Jane G Scarlett et al.
Biochimica et biophysica acta, 1708(1), 108-119 (2005-05-11)
Reduction of the cell-impermeable tetrazolium salt WST-1 has been used to characterise two plasma membrane NADH oxidoreductase activities in human cells. The trans activity, measured with WST-1 and the intermediate electron acceptor mPMS, utilises reducing equivalents from intracellular sources, while
Junpei Takaki et al.
Journal of neuroinflammation, 9, 275-275 (2012-12-25)
In the central nervous system, astrocytic L-glutamate (L-Glu) transporters maintain extracellular L-Glu below neurotoxic levels, but their function is impaired with neuroinflammation. Microglia become activated with inflammation; however, the correlation between activated microglia and the impairment of L-Glu transporters is
S Nakamura et al.
Clinica chimica acta; international journal of clinical chemistry, 101(2-3), 321-326 (1980-02-28)
1-Methoxy-5-methylphenazinium methyl sulfate (1-methoxyPMS) is a photochemically stable electron mediator between NAD(P)H and tetrazolium dyes. We have examined the efficiency of 1-methoxyPMS as an electron mediator in the assay of human lactate dehydrogenase (LDH, EC 1.1.1.27) and the activity staining
Patries M Herst et al.
Biochimica et biophysica acta, 1767(2), 170-177 (2007-02-03)
Oxygen consumption for bioenergetic purposes has long been thought to be the prerogative of mitochondria. Nevertheless, mitochondrial gene knockout (rho(0)) cells that are defective in mitochondrial respiration require oxygen for growth and consume oxygen at the cell surface via trans-plasma
Maxim Bychkov et al.
PloS one, 14(5), e0217339-e0217339 (2019-06-01)
Lynx1 is the first three-finger prototoxin found in the mammalian central nervous system. It is a GPI-anchored protein modulating nicotinic acetylcholine receptors (nAChRs) in the brain. Besides the brain, the Lynx1 protein was found in the lung and kidney. Endogenous
John R Ridpath et al.
Genes and environment : the official journal of the Japanese Environmental Mutagen Society, 41, 23-23 (2020-01-01)
Development of chemotherapeutic/preventive drugs that selectively kill cancer - the Holy Grail of cancer research - is a major challenge. A particular difficulty arises when chemotherapeutics and radiation are found to be rather ineffective against quiescent cancer cells in solid
Fernando Cartón-García et al.
Scientific reports, 5, 12312-12312 (2015-07-24)
Inherited MYO5B mutations have recently been associated with microvillus inclusion disease (MVID), an autosomal recessive syndrome characterized by intractable, life-threatening, watery diarrhea appearing shortly after birth. Characterization of the molecular mechanisms underlying this disease and development of novel therapeutic approaches
Elizabeth Gerard et al.
Pain, 156(7), 1320-1333 (2015-04-09)
Neuropathic pain is a chronic pain syndrome that arises from nerve injury. Current treatments only offer limited relief, clearly indicating the need for more effective therapeutic strategies. Previously, we demonstrated that proinflammatory tumor necrosis factor-alpha (TNF) is a key mediator
Zhaobin Zhang et al.
Nature communications, 8, 14585-14585 (2017-03-02)
Bisphenol A (BPA) is used in the production of plastic but has oestrogenic activity. Therefore, BPA substitutes, such as fluorene-9-bisphenol (BHPF), have been introduced for the production of so-called 'BPA-free' plastics. Here we show that BHPF is released from commercial
E Seidler
Acta histochemica, 66(2), 273-275 (1980-01-01)
1-Methoxy-5-methylphenazinium methyl sulfate (MPMS) was tested for use as redox mediator in histochemistry of dehydrogenases. Aqueous solutions of MPMS are absolute lightstable. Like PMS or Meldola Blue the succinate dehydrogenase reaction was increased by MPMS. In an non-enzymatic NADH-NBT-test the
Methods in Plant Cell Biology (1995)
Xingyi Ma et al.
The Analyst, 137(14), 3328-3334 (2012-06-07)
We developed a simple, ultrasensitive, and quantitative detection method for the reduced form of nicotinamide adenine dinucleotide (NADH), based on carbon nanotube field effect transistors (CNTFETs). Following the injection of NADH at different concentrations, we obtained different electrical signals from
Early life exposure to chronic intermittent hypoxia causes upper airway dilator muscle weakness, which persists into young adulthood.
McDonald FB, et. al.
Experimental Physiology, 100, 947-947 (2015)
Xu Tian et al.
PloS one, 11(12), e0167503-e0167503 (2016-12-03)
Contamination of potentially carcinogenic hexavalent chromium (Cr(VI)) in the drinking water is a major public health concern worldwide. However, little information is available regarding the biological effects of a nanomoler amount of Cr(VI). Here, we investigated the genotoxic effects of
Yuta Mutaguchi et al.
Analytical biochemistry, 409(1), 1-6 (2010-10-19)
Methods with which to simply and rapidly assay L-aspartate (L-Asp) and D-aspartate (D-Asp) would be highly useful for physiological research and for nutritional and clinical analyses. Levels of L- and D-Asp in food and cell extracts are currently determined using
Kumarasamy Murali et al.
Nanoscale, 7(9), 4199-4210 (2015-02-13)
Because of their capacity of crossing an intact blood-brain barrier and reaching the brain through an injured barrier or via the nasal epithelium, nanoparticles have been considered as vehicles to deliver drugs and as contrast materials for brain imaging. The
An optimized lactate dehydrogenase release assay for screening of drug candidates in neuroscience.
Kaja S et al.
Journal of Pharmacological and Toxicological Methods, 73, 1-1 (2015)
Toshiko Yamamoto et al.
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, 22(7), 793-797 (2002-08-20)
We have developed an assay system for guinea pig interferon (IFN) based on reduction of viral cytopathic effect (CPE) in various cell lines. CPE inhibition was detected optimally in the guinea pig fibroblast cell line 104C1 infected with encephalomyocarditis virus
Anne Miller et al.
Cell metabolism, 26(5), 788-800 (2017-09-12)
Over the past years, plenty of evidence has emerged illustrating how metabolism supports many aspects of cellular function and how metabolic reprogramming can drive cell differentiation and fate. Here, we present a method to assess the metabolic configuration of single
Petra A Priemel et al.
The Journal of pharmacy and pharmacology, 70(8), 1009-1021 (2018-06-01)
Pulmonary delivery of antibiotics will decrease the required dose for efficient treatment of lung infections and reduce systemic side effects of the drug. The objective was to evaluate the applicability of poly(ethylene carbonate) (PEC) for the preparation of inhalable, antibiotic-containing
Kuniyo Inouye et al.
Journal of biochemistry, 131(1), 97-105 (2002-01-05)
A synchronous enzyme-reaction system using water-soluble formazan and a non-enzymatic electron mediator was developed and applied to an enzyme immunoassay (EIA). The reaction system consists of four steps: (I) dephosphorylation of NADP(+) to produce NAD(+) by alkaline phosphatase (ALP), (II)
An S Tan et al.
Redox report : communications in free radical research, 9(6), 302-306 (2005-02-22)
Trans-plasma membrane electron transport (tPMET) in mammalian cells has been demonstrated using artificial cell-impermeable dyes, but the extent to which reduction of these dyes involves distinct pathways remains unclear. Here we compare the properties of three commonly used dyes, WST-1
Hiroaki Okuda et al.
Neurochemical research, 41(1-2), 278-289 (2015-12-24)
Sonic hedgehog (Shh), a member of the Hedgehog (Hh) family, plays essential roles in the development of the central nervous system. Recent studies suggest that the Hh signaling pathway also functions in mature astrocytes under physiological conditions. We first examined
Fonnet E Bleeker et al.
Acta neuropathologica, 119(4), 487-494 (2010-02-04)
Somatic mutations in the isocitrate dehydrogenase 1 gene (IDH1) occur at high frequency in gliomas and seem to be a prognostic factor for survival in glioblastoma patients. In our set of 98 glioblastoma patients, IDH1 ( R132 ) mutations were
Kazumichi Ozawa et al.
Applied microbiology and biotechnology, 101(1), 173-183 (2016-08-12)
FAD-dependent glucose dehydrogenase (FAD-GDH), which contains FAD as a cofactor, catalyzes the oxidation of D-glucose to D-glucono-1,5-lactone, and plays an important role in biosensors measuring blood glucose levels. In order to obtain a novel FAD-GDH gene homolog, we performed degenerate
T Nakaminami et al.
Analytical chemistry, 71(19), 4278-4283 (1999-10-12)
A biomimetic bilayer membrane immobilizing uricase (urate oxidase; EC 1.7.3.3) (UOx) and a redox agent of 1-methoxy-5-methylphenazinium (MMP) was fabricated on an Au electrode substrate with use of the Au substrate coated with a self-assembled monolayer of n-octanethiolate (OT/Au) and
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