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Gary S L Peh et al.
Scientific reports, 5, 9167-9167 (2015-04-01)
The global shortage of donor corneas has garnered extensive interest in the development of graft alternatives suitable for endothelial keratoplasty using cultivated primary human corneal endothelial cells (CECs). We have recently described a dual media approach for the propagation of
Monika Gibis et al.
Journal of food science, 80(11), C2430-C2439 (2015-10-08)
The impact of precursors such as creatine, creatinine, and glucose on the formation of mutagenic/carcinogenic heterocyclic amines (HAs) were studied in patties of 9 different animal species equally heat treated with a double-plate contact grill. All grilled patties of the
Mark A Travassos et al.
The American journal of tropical medicine and hygiene, 93(2), 416-424 (2015-06-10)
A community-based immunization coverage survey is the standard way to estimate effective vaccination delivery to a target population in a region. Accompanying serosurveys can provide objective measures of protective immunity against vaccine-preventable diseases but pose considerable challenges with respect to
Helen L Wright et al.
Rheumatology (Oxford, England), 54(1), 188-193 (2014-08-16)
The aim of this study was to use whole transcriptome sequencing (RNA-Seq) of RA neutrophils to identify pre-therapy gene expression signatures that correlate with disease activity or response to TNF inhibitor (TNFi) therapy. Neutrophils were isolated from the venous blood
Hui-Ling Che et al.
International journal of analytical chemistry, 2015, 357609-357609 (2015-11-26)
Quantification of tocotrienols in human plasma is critical when the attention towards tocotrienols on its distinctive properties is arising. We aim to develop a simple and practical normal-phase high performance liquid chromatography method to quantify the amount of four tocotrienol
Caroline Übel et al.
Scientific reports, 4, 5865-5865 (2014-08-12)
In a murine model of allergic asthma, we found that Tyk-2((-/-)) asthmatic mice have induced peribronchial collagen deposition, mucosal type mast cells in the lung, IRF4 and hyperproliferative lung Th2 CD4(+) effector T cells over-expressing IL-3, IL-4, IL-5, IL-10 and
Jette Bork-Jensen et al.
Diabetologia, 58(2), 363-373 (2014-11-19)
We aimed to identify microRNAs (miRNAs) associated with type 2 diabetes and risk of developing the disease in skeletal muscle biopsies from phenotypically well-characterised twins. We measured muscle miRNA levels in monozygotic (MZ) twins discordant for type 2 diabetes using
M Ghosh et al.
Animal genetics, 46(3), 255-264 (2015-03-11)
This study was carried out with the objective to investigate the differentially expressed genes (DEGs) between Jeju native pig (JNP) and Berkshire piglets. The RNA-Seq technique was used to investigate the transcriptomes in the fat, liver and longissimus dorsi muscle
Yan-Ting Wu et al.
Journal of assisted reproduction and genetics, 32(3), 417-427 (2015-01-18)
To study the differences in protein expression profiles of follicular fluid (FF) between controlled ovarian hyperstimulation (COH) and natural ovulatory cycles. Twelve infertile women undergoing in vitro fertilization and embryo transfer (IVF-ET), with matched clinical information, were retrospectively recruited in
Jasmin Krismer et al.
Applied and environmental microbiology, 81(16), 5546-5551 (2015-06-07)
The consequences of cellular heterogeneity, such as biocide persistence, can only be tackled by studying each individual in a cell population. Fluorescent tags provide tools for the high-throughput analysis of genomes, RNA transcripts, or proteins on the single-cell level. However
Anders Norman et al.
PloS one, 9(8), e104405-e104405 (2014-08-15)
Two recently developed isolation methods have shown promise when recovering pure community plasmid DNA (metamobilomes/plasmidomes), which is useful in conducting culture-independent investigations into plasmid ecology. However, both methods employ multiple displacement amplification (MDA) to ensure suitable quantities of plasmid DNA
Agnieszka Chabowska-Kita et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 29(8), 3238-3252 (2015-04-22)
The brown adipocyte phenotype (BAP) in white adipose tissue (WAT) is transiently induced in adult mammals in response to reduced ambient temperature. Since it is unknown whether a cold challenge can permanently induce brown adipocytes (BAs), we reared C57BL/6J (B6)
Damian Cordoba Diaz et al.
Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.], 39(9), 1363-1367 (2013-05-04)
A phenolization approach is often chosen for treatment of ingrown toenails. Many reports describe lavage of the wound with alcohol to neutralize any residual phenol. The aim of our study was to compare the suitability and effectiveness of a solution
Wei-Dong Xu et al.
Journal of proteomics, 113, 57-72 (2014-10-05)
The present study first utilized a standardized shotgun proteomic analysis method to determine differences in protein expression of fibroblasts in the ligament between AS patients and healthy controls. Proteins extracted from primarily cultured FLLs from 35 AS patients and 10
Piia Takabe et al.
Experimental cell research, 337(1), 1-15 (2015-07-30)
Malignant skin melanoma is one of the most deadly human cancers. Extracellular matrix (ECM) influences the growth of malignant tumors by modulating tumor cells adhesion and migration. Hyaluronan is an essential component of the ECM, and its amount is altered
Alexandra Jaeger et al.
Veterinary research, 46, 50-50 (2015-05-08)
Postpartum Dysgalactia Syndrome (PDS) represents a considerable health problem of postpartum sows, primarily indicated by mastitis and lactation failure. The poorly understood etiology of this multifactorial disease necessitates the use of the porcine mammary epithelial cell (PMEC) model to identify
Joanna B Aitken et al.
Biology of reproduction, 92(5), 125-125 (2015-03-06)
This study demonstrates for the first time the presence of an L-amino acid oxidase (LAAO) enzyme in equine spermatozoa that is able to generate significant amounts of reactive oxygen species (ROS) and create a state of oxidative stress. RT-PCR analysis
Dmitri E Sharifulin et al.
Biochimica et biophysica acta, 1849(8), 930-939 (2015-06-13)
In this work, intimate contacts of riboses of mRNA stretch from nucleotides in positions +3 to +12 with respect to the first nucleotide of the P site codon were studied using cross-linking of short mRNA analogs with oxidized 3'-terminal riboses
Neil L Berinstein et al.
Oncoimmunology, 4(8), e1026529-e1026529 (2015-09-26)
DepoVax™ is an innovative and strongly immunogenic vaccine platform. Survivin is highly expressed in many tumor types and has reported prognostic value. To generate tumor-specific immune response, a novel cancer vaccine was formulated in DepoVax platform (DPX-Survivac) using survivin HLA
Yan Li et al.
BMC cancer, 15, 417-417 (2015-05-20)
DNA methylation regulates gene expression, through the inhibition/activation of gene transcription of methylated/unmethylated genes. Hence, DNA methylation profiling can capture pivotal features of gene expression in cancer tissues from patients at the time of diagnosis. In this work, we analyzed
Luís D R Melo et al.
Research in microbiology, 165(8), 679-685 (2014-10-12)
Relatively few phages (<10) of coagulase negative staphylococci (CoNS) have been described. Staphylococcus epidermidis phage vB_SepS_SEP9 is a siphovirus with a unique morphology as a staphylococcal phage, possessing a very long tail. Its genome is unique and unrelated to any
Zhang Zhao et al.
Oncology letters, 9(6), 2839-2846 (2015-07-03)
Chromosomal loci with genomic imbalances are frequently identified in hepatocellular carcinoma (HCC). Greater than two-thirds of hepatitis B virus (HBV)-related HCCs originate from liver cirrhosis following a duration of up to two decades. However, it is unclear whether these genomic
N Craig Carville et al.
Journal of biomedical materials research. Part A, 103(8), 2540-2548 (2014-12-17)
In this work, the influence of substrate surface charge on in vitro osteoblast cell proliferation on ferroelectric lithium niobate (LN) crystal surfaces is investigated. LN has a spontaneous polarization along the z-axis and is thus characterized by positive and negative
Lok Kumar Shrestha et al.
Journal of the American Chemical Society, 135(2), 586-589 (2013-01-02)
A new class of fullerene (C(60)) crystals with bimodal pore architectures consisting of macropores and mesopores was synthesized by using a liquid-liquid interfacial precipitation (LLIP) method involving an interface between isopropyl alcohol (IPA) and a saturated solution of C(60) in
Yuying Zhang et al.
Physical chemistry chemical physics : PCCP, 17(33), 21120-21126 (2014-12-11)
Noble metal nanoparticles (NPs) are the most commonly employed plasmonic substrates in surface-enhanced Raman scattering (SERS) experiments. Computer simulations show that monomers of Ag and Au nanocrystals ("spherical" NPs) do not exhibit a notable plasmonic enhancement, i.e., they are essentially
Narendranath Vikkath et al.
Familial cancer, 14(4), 585-594 (2015-05-09)
The general prevalence of the familial multi-organ tumor disorder, von Hippel-Lindau syndrome (VHL), was estimated to be 1 in 25-40,000 in western studies two decades back. Few studies were done in Indian sub-continent, amidst a surge in clinical reports on
Simone Wahl et al.
BMC medicine, 13, 48-48 (2015-04-11)
Excess body weight is a major risk factor for cardiometabolic diseases. The complex molecular mechanisms of body weight change-induced metabolic perturbations are not fully understood. Specifically, in-depth molecular characterization of long-term body weight change in the general population is lacking.
Sara Triñanes et al.
Talanta, 132, 433-442 (2014-12-06)
A new straightforward and inexpensive sample screening method for both EPA and EU priority polycyclic aromatic hydrocarbons (PAHs) in water has been developed. The method is based on combined direct immersion and headspace (DIHS) sorptive extraction, using low-cost disposable material
Ulrich-Christian Schröder et al.
Biomicrofluidics, 9(4), 044118-044118 (2015-09-05)
This work presents a polymeric centrifugal microfluidic platform for the rapid and sensitive identification of bacteria directly from urine, thus eliminating time-consuming cultivation steps. This "Lab-on-a-Disc" platform utilizes the rotationally induced centrifugal field to efficiently capture bacteria directly from suspension
Julie Lessard et al.
PloS one, 10(3), e0122065-e0122065 (2015-03-31)
Mature adipocytes can reverse their phenotype to become fibroblast-like cells. This is achieved by ceiling culture and the resulting cells, called dedifferentiated fat (DFAT) cells, are multipotent. Beyond the potential value of these cells for regenerative medicine, the dedifferentiation process
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