Showing 1-30 of 101 results for "

I8896

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Anthony W Purcell et al.
Nature protocols, 14(6), 1687-1707 (2019-05-17)
Peptide antigens bound to molecules encoded by the major histocompatibility complex (MHC) and presented on the cell surface form the targets of T lymphocytes. This critical arm of the adaptive immune system facilitates the eradication of pathogen-infected and cancerous cells...
Heinz Fischer et al.
PloS one, 6(3), e17581-e17581 (2011-03-11)
The cornified layer, the stratum corneum, of the epidermis is an efficient barrier to the passage of genetic material, i.e. nucleic acids. It contains enzymes that degrade RNA and DNA which originate from either the living part of the epidermis...
Ana C A M Pajuaba et al.
Proteomics, 12(6), 820-831 (2012-04-28)
Brucella abortus is a Gram-negative intracellular bacterium that causes infectious abortion in food-producing animals and chronic infection in humans. This study aimed to characterize a B. abortus S19 antigen preparation obtained by Triton X-114 (TX-114) extraction through immunoproteomics to differentiate...
Yasmin M Vasquez et al.
Molecular endocrinology (Baltimore, Md.), 29(3), 421-433 (2015-01-15)
The forkhead box O1A (FOXO1) is an early-induced target of the protein kinase A pathway during the decidualization of human endometrial stromal cells (HESCs). In this study we identified the cistrome and transcriptome of FOXO1 and its role as a...
R Lappe et al.
Natural product research, 26(23), 2259-2265 (2012-01-21)
Cerein 8A is an antimicrobial peptide with potential application against food spoilage and pathogenic bacteria. The partitioning of cerein 8A was investigated in two liquid-liquid extraction systems that are considered promising for bioseparation and purification purposes. Aqueous two-phase systems (ATPSs)...
Dorthe Helena Larsen et al.
The Journal of cell biology, 190(5), 731-740 (2010-09-02)
In response to ionizing radiation (IR), cells delay cell cycle progression and activate DNA repair. Both processes are vital for genome integrity, but the mechanisms involved in their coordination are not fully understood. In a mass spectrometry screen, we identified...
Matteo Colombo et al.
Virology journal, 6, 59-59 (2009-05-19)
The genome of measles virus consists of a non-segmented single-stranded RNA molecule of negative polarity, which is encapsidated by the viral nucleoprotein (N) within a helical nucleocapsid. The N protein possesses an intrinsically disordered C-terminal domain (aa 401-525, N(TAIL)) that...
Yoshinori Asano et al.
Scientific reports, 6, 19174-19174 (2016-01-13)
The transcription factors HSF1 and p53 both modulate the stress response, thereby protecting and facilitating the recovery of stressed cells, but both have the potential to promote tumor development. Here we show that a p53 target gene, IER5, encodes an...
Mariane H Schleimann et al.
EBioMedicine, 45, 328-340 (2019-07-14)
TLR9 agonists are being developed as immunotherapy against malignancies and infections. TLR9 is primarily expressed in B cells and plasmacytoid dendritic cells (pDCs). TLR9 signalling may be critically important for B cell activity in lymph nodes but little is known...
Silvia Castagnaro et al.
Autophagy, 12(12), 2484-2495 (2016-09-23)
A pilot clinical trial based on nutritional modulation was designed to assess the efficacy of a one-year low-protein diet in activating autophagy in skeletal muscle of patients affected by COL6/collagen VI-related myopathies. Ullrich congenital muscular dystrophy and Bethlem myopathy are...
Per Wasteson et al.
Development (Cambridge, England), 135(10), 1823-1832 (2008-04-18)
Aortic smooth muscle cells (SMCs) have been proposed to derive from lateral plate mesoderm. It has further been suggested that induction of SMC differentiation is confined to the ventral side of the aorta, and that SMCs later migrate to the...
Shikha Gupta et al.
Journal of pharmaceutical and biomedical analysis, 66, 33-39 (2012-04-03)
A new validated high-performance thin-layer chromatographic (HPTLC) method has been developed for the simultaneous quantitation of four antipsychotic indole alkaloids (IAs), reserpiline (RP, 1), α-yohimbine (YH, 2), isoreserpiline (IRP, 3) and 10-methoxy tetrahydroalstonine (MTHA, 4) as markers in the leaves...
Bojana Lucic et al.
Nature communications, 10(1), 4059-4059 (2019-09-08)
HIV-1 recurrently targets active genes and integrates in the proximity of the nuclear pore compartment in CD4+ T cells. However, the genomic features of these genes and the relevance of their transcriptional activity for HIV-1 integration have so far remained...
Hamta Shoaee et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 98, 70-75 (2012-09-18)
A cloud-point extraction process coupled to ICP-OES by using 3-nitro benzaldehyde thiosemicarbazone (3-NBT) as complexing agent was developed for the simultaneous preconcentration and determination of copper and mercury in water samples. The variables affecting the complexation and extraction steps were...
Junjie Tony Hua et al.
Cell, 174(3), 564-575 (2018-07-24)
The prostate cancer (PCa) risk-associated SNP rs11672691 is positively associated with aggressive disease at diagnosis. We showed that rs11672691 maps to the promoter of a short isoform of long noncoding RNA PCAT19 (PCAT19-short), which is in the third intron of...
Ariel C Aspiras et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(31), 9668-9673 (2015-07-15)
Despite recent advances in the understanding of morphological evolution, the genetic underpinnings of behavioral and physiological evolution remain largely unknown. Here, we study the metabolic changes that evolved in independently derived populations of the Mexican cavefish, Astyanax mexicanus. A hallmark...
Qi Zhao et al.
Journal of chromatographic science, 51(4), 341-348 (2012-09-15)
A method based on cloud point extraction (CPE) coupled with high-performance liquid chromatography separation and ultraviolet (UV) detection was developed to determine andrographolide and dehydroandrographolide in human plasma. The nonionic surfactant Triton X-114 was chosen as the extraction medium. Variable...
Stuart K Archer et al.
PLoS pathogens, 5(8), e1000565-e1000565 (2009-08-29)
Many genes that are required at specific points in the cell cycle exhibit cell cycle-dependent expression. In the early-diverging model eukaryote and important human pathogen Trypanosoma brucei, regulation of gene expression in the cell cycle and other processes is almost...
Anna Hitrik et al.
PLoS genetics, 12(11), e1006330-e1006330 (2016-11-16)
The development of niches for tissue-specific stem cells is an important aspect of stem cell biology. Determination of niche size and niche numbers during organogenesis involves precise control of gene expression. How this is achieved in the context of a...
Teruyuki Fukushima et al.
BMC neuroscience, 12, 36-36 (2011-05-10)
Enkephalins are endogenous opiates that are assumed to modulate nociceptive information by mediating synaptic transmission in the central nervous system, including the spinal dorsal horn. To develop a new tool for the identification of in vitro enkephalinergic neurons and to...
Kenneth Shatzkes et al.
Scientific reports, 4, 4659-4659 (2014-04-12)
Sample nucleic acid purification can often be rate-limiting for conventional quantitative PCR (qPCR) workflows. We recently developed high-throughput virus microneutralization assays using an endpoint assessment approach based on reverse transcription qPCR (RT-qPCR). The need for cumbersome RNA purification is circumvented...
Chao Niu et al.
Autophagy, 15(5), 843-870 (2019-01-18)
Studies regarding macroautophagic/autophagic regulation in endothelial cells (ECs) under diabetic conditions are very limited. Clinical evidence establishes an endothelial protective effect of metformin, but the underlying mechanisms remain unclear. We aimed to investigate whether metformin exerts its protective role against...
Margarita Cabrera et al.
The Journal of cell biology, 191(4), 845-859 (2010-11-17)
Tethering factors are organelle-specific multisubunit protein complexes that identify, along with Rab guanosine triphosphatases, transport vesicles and trigger their SNARE-mediated fusion of specific transport vesicles with the target membranes. Little is known about how tethering factors discriminate between different trafficking...
Friedrich P Thinnes et al.
Molecular genetics and metabolism, 107(3), 632-633 (2012-09-25)
There is indication that human type-1 VDAC/Porin31HL complexes, when purified from highly enriched cell membrane preparations of human B-lymphocytes by classical ion-exchange chromatography in the detergent Nonidet P40, rest in fully closed state, its N-terminus being accessible for mAbs. Cholesterol...
Dimosthenis L Giokas et al.
Journal of chromatography. A, 1251, 33-39 (2012-07-13)
A novel two-step extraction technique combining cloud point extraction (CPE) with dispersive micro-solid phase extraction (D-μ-SPE) is presented in this work for the first time. The method involves initial extraction of the target analytes by CPE in the micelles of...
Amr Ali Mohamed et al.
Journal of colloid and interface science, 381(1), 198-201 (2012-06-19)
Uniform fully dispersed particles of flufenamic acid, a widely used anti-inflammatory drug, were prepared by two different methods. In the first one, the drug solution in organic solvents was added to a non-solvent (water or aqueous solutions of stabilizers); while...
Julian Nüchel et al.
Autophagy, 14(3), 465-486 (2018-01-04)
TGFB1 (transforming growth factor beta 1) is a potent cytokine playing a driving role in development, fibrosis and cancer. It is synthesized as prodomain-growth factor complex that requires tethering to LTBP (latent transforming growth factor beta binding protein) for efficient...
Adam J Lowe et al.
Methods in molecular biology (Clifton, N.J.), 899, 265-275 (2012-06-28)
Endotoxin removal is critical when producing therapeutic proteins in bacterial systems. This hydrophobic compound can be removed through chromatography or filtration, but presents unique challenges dependent upon protein composition as well as production scale. Here we present a robust method...
Suman Rao et al.
Cell chemical biology, 26(6), 818-829 (2019-04-16)
Covalent kinase inhibitors, which typically target cysteine residues, represent an important class of clinically relevant compounds. Approximately 215 kinases are known to have potentially targetable cysteines distributed across 18 spatially distinct locations proximal to the ATP-binding pocket. However, only 40...
Chunyapuk Kukusamude et al.
Journal of chromatography. A, 1351, 110-114 (2014-06-09)
Sample clean-up by cloud point phase separation and analysis by capillary electrophoresis with stacking was developed for quaternary ammonium herbicides (i.e., paraquat and diquat) in milk. For sample clean-up, a mixture of 845μL of milk sample, 5μL of 100mM phosphoric...