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Tali H Reingewertz et al.
Biochemistry, 54(21), 3337-3347 (2015-05-13)
The leading risk factor for gastric cancer in humans is infection by Helicobacter pylori strains that express and translocate the oncoprotein CagA into host epithelial cells. Once inside host cells, CagA interacts with ASPP2, which specifically stimulates p53-mediated apoptosis and
Avisek Mahapa et al.
PloS one, 10(3), e0122168-e0122168 (2015-03-31)
SarA, a Staphylococcus aureus-specific dimeric protein, modulates the expression of numerous proteins including various virulence factors. Interestingly, S. aureus synthesizes multiple SarA paralogs seemingly for optimizing the expression of its virulence factors. To understand the domain structure/flexibility and the folding/unfolding
B K Kishore Reddy et al.
Antimicrobial agents and chemotherapy, 58(6), 3312-3326 (2014-04-02)
Pantothenate kinase (PanK) catalyzes the phosphorylation of pantothenate, the first committed and rate-limiting step toward coenzyme A (CoA) biosynthesis. In our earlier reports, we had established that the type I isoform encoded by the coaA gene is an essential pantothenate
Xuguo Duan et al.
Applied and environmental microbiology, 81(6), 1926-1931 (2015-01-04)
Pullulanase (EC 3.2.1.41), an important enzyme in the production of starch syrup, catalyzes the hydrolysis of α-1,6 glycosidic bonds in complex carbohydrates. A double mutant (DM; D437H/D503Y) form of Bacillus deramificans pullulanase was recently constructed to enhance the thermostability and
Hang Yang et al.
Scientific reports, 5, 17257-17257 (2015-11-27)
The increasing emergence of multi-drug resistant streptococci poses a serious threat to public health worldwide. Bacteriophage lysins are promising alternatives to antibiotics; however, their narrow lytic spectrum restricted to closely related species is a central shortcoming to their translational development.
Mona I Shaaban et al.
Journal of microbiology and biotechnology, 25(6), 887-892 (2015-01-16)
Uricase is an important microbial enzyme that can be used in the clinical treatment of gout, hyperuricemia, and tumor lysis syndrome. A total of 127 clinical isolates of Pseudomonas aeruginosa were tested for uricase production. A Pseudomonas strain named Ps43
Kelly E Miller et al.
The Journal of cell biology, 209(2), 211-220 (2015-04-22)
Linker histone H1 is required for mitotic chromosome architecture in Xenopus laevis egg extracts and, unlike core histones, exhibits rapid turnover on chromatin. Mechanisms regulating the recruitment, deposition, and dynamics of linker histones in mitosis are largely unknown. We found
Soon-Kyeong Kwon et al.
Scientific reports, 5, 16076-16076 (2015-11-05)
Achieving sufficient yields of proteins in their functional form represents the first bottleneck in contemporary bioscience and biotechnology. To accomplish successful overexpression of membrane proteins in a workhorse organism such as E. coli, defined and rational optimization strategies based on
Astrid Pérez et al.
The Journal of antimicrobial chemotherapy, 69(9), 2407-2411 (2014-05-23)
Two natural variants of ADC-type β-lactamases of Acinetobacter spp., ADC-1 and ADC-5, differ by nine mutations in their protein sequence. ADC-5 hydrolyses cefoxitin better than ADC-1 and the opposite is true for ceftazidime. We produced single and combined mutations in
Coilín Boland et al.
Cellular and molecular life sciences : CMLS, 71(24), 4895-4910 (2014-07-12)
Membrane proteins are key elements in cell physiology and drug targeting, but getting a high-resolution structure by crystallographic means is still enormously challenging. Novel strategies are in big demand to facilitate the structure determination process that will ultimately hasten the
Craig Skinner et al.
PloS one, 10(7), e0132419-e0132419 (2015-07-21)
Stx2e is a primary virulence factor in STEC strains that cause edema disease in neonatal piglets. Though Stx2a and Stx2e are similar, many antibody-based Stx detection kits are designed to detect Stx2a and do not recognize the Stx2e subtype. Four
Andreas Bracher et al.
PloS one, 10(8), e0135448-e0135448 (2015-08-26)
The most prevalent form of the Rubisco enzyme is a complex of eight catalytic large subunits (RbcL) and eight regulatory small subunits (RbcS). Rubisco biogenesis depends on the assistance by specific molecular chaperones. The assembly chaperone RbcX stabilizes the RbcL
Na Feng et al.
Biochemical and biophysical research communications, 459(3), 481-487 (2015-03-10)
Both mutation and knockdown in mev-1 gene render Caenorhabditis elegans susceptibility to Enterococcus faecalis infection. However, the mechanisms by which of MEV-1 defects pathogen resistance remain unclear. Here we show that mev-1RNAi causes a dramatic decrease in oxidative stress and
Hsin-Wei Liao et al.
The Journal of clinical investigation, 125(12), 4529-4543 (2015-11-17)
Posttranslational modifications to the intracellular domain of the EGFR are known to regulate EGFR functions; however, modifications to the extracellular domain and their effects remain relatively unexplored. Here, we determined that methylation at R198 and R200 of the EGFR extracellular
Rajib Schubert et al.
FEBS letters, 588(19), 3639-3648 (2014-06-15)
Single-cell force spectroscopy (SCFS) is becoming a widely used method to quantify the adhesion of a living cell to a substrate, another cell or tissue. The high sensitivity of SCFS permits determining the contributions of individual cell adhesion molecules (CAMs)
Pushkar Malakar et al.
Archives of microbiology, 195(4), 291-295 (2013-01-29)
Studies have shown that the production of unnecessary proteins burdens the cellular growth mainly due to allocation of cellular resources to unnecessary protein synthesis, thereby limiting the resources available for growth. In the current study, we focus on the effect
Hamidreza Iranpoor et al.
Avicenna journal of medical biotechnology, 7(3), 101-105 (2015-08-26)
Human insulin-like growth factor type 1 (hIGF-1) is a protein consisting of 70 amino acids (MW=7.6 kDa) and mainly synthesized by liver. Mecasermin (Trade name INCRELEX) is the synthetic form of the protein which is used as an effective treatment
Se Hee Han et al.
Cell & bioscience, 5, 50-50 (2015-08-28)
Human DNA topoisomerase II-binding protein 1 (hTopBP1) plays an important role in DNA replication and the DNA damage checkpoint pathway. The human mutY homolog (hMYH) is a base excision repair DNA glycosylase that excises adenines or 2-hydroxyadenines that are mispaired
Don Anushka Sandaruwan Elvitigala et al.
Developmental and comparative immunology, 53(1), 169-178 (2015-07-18)
Pentraxins are a family of evolutionary conserved proteins that contains two main members, namely c-reactive proteins (CRPs) and serum amyloid P (SAP), which are involved in acute phase responses in animals. In this study, a cDNA sequence of a CRP-like
Benjamin F Zhao et al.
Biochemical and biophysical research communications, 453(4), 761-766 (2014-12-03)
Protein tyrosine phosphorylation is thought to be a unique feature of multicellular animals. Interestingly, the genome of the unicellular protist Monosiga brevicollis reveals a surprisingly high number and diversity of protein tyrosine kinases, protein tyrosine phosphatases (PTPs), and phosphotyrosine-binding domains.
Qingxin Li et al.
Scientific reports, 5, 13586-13586 (2015-09-01)
Erythropoiesis is regulated by the erythropoietin receptor (EpoR) binding to its ligand. The transmembrane domain (TMD) and the juxtamembrane (JM) regions of the EpoR are important for signal transduction across the cell membrane. We report a solution NMR study of
Mustafa T Ardah et al.
Neurobiology of disease, 74, 89-101 (2014-12-03)
Compelling evidence indicates that α-synuclein (α-syn) aggregation plays a central role in the pathogenesis of Parkinson's disease (PD) and other synucleinopathies. Identification of compounds that inhibit or reverse the aggregation process may thus represent a viable therapeutic strategy against PD
Jernej Godec et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(2), 512-517 (2014-12-31)
The differentiation of effector CD8(+) T cells is critical for the development of protective responses to pathogens and for effective vaccines. In the first few hours after activation, naive CD8(+) T cells initiate a transcriptional program that leads to the
Anna Starus et al.
Biochemistry, 54(31), 4834-4844 (2015-07-18)
Binding of the competitive inhibitor L-captopril to the dapE-encoded N-succinyl-L,L-diaminopimelic acid desuccinylase from Neisseria meningitidis (NmDapE) was examined by kinetic, spectroscopic, and crystallographic methods. L-Captopril, an angiotensin-converting enzyme (ACE) inhibitor, was previously shown to be a potent inhibitor of the
Valerie Stadlmann et al.
BMC biotechnology, 15, 109-109 (2015-12-03)
Celiac disease (CD) is a chronic, small intestinal inflammatory disease mediated by dietary gluten and related prolamins. The only current therapeutic option is maintenance of a strict life-long gluten-free diet, which implies substantial burden for CD patients. Different treatment regimes
Farzad Badmasti et al.
Journal of medical microbiology, 64(Pt 5), 559-564 (2015-03-31)
Acinetobacter baumannii is a Gram-negative bacteria associated with hospital-acquired infections. Definitely, antimicrobial resistance and biofilm formation capabilities of clinical isolates have threading potential to persistence in the hospital environment and colonization on medical equipment. Twenty-seven multidrug-resistant clinical isolates were selected
Haizhan Jiao et al.
Structure (London, England : 1993), 27(8), 1258-1269 (2019-06-11)
In mitochondria, CDP-diacylglycerol (CDP-DAG) is a crucial precursor for cardiolipin biosynthesis. Mitochondrial CDP-DAG is synthesized by the translocator assembly and maintenance protein 41 (Tam41) through an elusive process. Here we show that Tam41 adopts sequential catalytic mechanism, and report crystal
Duraisamy Ponnusamy et al.
PloS one, 10(11), e0141984-e0141984 (2015-11-05)
Yersinia pestis initiates infection by parasitism of host macrophages. In response to macrophage infections, intracellular Y. pestis can assume a filamentous cellular morphology which may mediate resistance to host cell innate immune responses. We previously observed the expression of Y.
Lorraine Endersen et al.
The Journal of general virology, 96(Pt 2), 463-477 (2014-11-06)
Bacteriophages and their derivatives are continuously gaining impetus as viable alternative therapeutic agents to control harmful multidrug-resistant bacterial pathogens, particularly in the food industry. The reduced efficacy of conventional antibiotics has resulted in a quest to find novel alternatives in
David R Cooper et al.
Biophysical journal, 109(1), 66-75 (2015-07-15)
The N-methyl-D-aspartate receptor (NMDAR) is a member of the glutamate receptor family of proteins and is responsible for excitatory transmission. Activation of the receptor is thought to be controlled by conformational changes in the ligand binding domain (LBD); however, glutamate
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