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Bennett J Davenport et al.
PLoS neglected tropical diseases, 12(10), e0006921-e0006921 (2018-10-30)
Leishmania species are sand fly-transmitted protozoan parasites that cause leishmaniasis, neglected tropical diseases that affect millions of people. Leishmania amastigotes must overcome a variety of host defenses, including reactive oxygen species (ROS) produced by the NADPH oxidase. Leishmania species encode
Victor Heurtier et al.
Nature communications, 10(1), 1109-1109 (2019-03-09)
Transcription factor networks, together with histone modifications and signalling pathways, underlie the establishment and maintenance of gene regulatory architectures associated with the molecular identity of each cell type. However, how master transcription factors individually impact the epigenomic landscape and orchestrate
Hong-Jay Lo et al.
Organic letters, 14(23), 5896-5899 (2012-11-15)
A chiral pool based synthetic strategy that leads from the readily available and inexpensive C(2)-symmetric tartaric acids to the chiral O-isopropylidenebenzooxazole--a convenient precursor to the aminocyclitol core of hygromycin A as well as the chiral γ-disilyloxybutyrolactone--a pivotal intermediate to approach
Iksung Jin et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(23), 9131-9136 (2012-05-24)
Long-term plasticity can differ from short-term in recruiting the growth of new synaptic connections, a process that requires the participation of both the presynaptic and postsynaptic components of the synapse. How does information about synaptic plasticity spread from its site
N Kretschy et al.
British journal of cancer, 108(3), 570-578 (2013-01-10)
As metastasis is the prime cause of death from malignancies, there is vibrant interest to discover options for the management of the different mechanistic steps of tumour spreading. Some approved pharmaceuticals exhibit activities against diseases they have not been developed
António J Pereira et al.
Methods in molecular biology (Clifton, N.J.), 545, 145-164 (2009-05-29)
Progress from our present understanding of the mechanisms behind mitosis has been compromised by the fact that model systems that were ideal for molecular and genetic studies (such as yeasts, C. elegans, or Drosophila) were not suitable for intracellular micromanipulation.
Bettina Fussbroich et al.
PloS one, 6(7), e21651-e21651 (2011-07-19)
The Enhancer of Zeste 2 (EZH2) protein has been reported to stimulate cell growth in some cancers and is therefore considered to represent an interesting new target for therapeutic intervention. Here, we investigated a possible role of EZH2 for the
Faisal Asghar Khattak et al.
BMC microbiology, 12, 204-204 (2012-09-13)
The genus Mycobacterium (M.) comprises highly pathogenic bacteria such as M. tuberculosis as well as environmental opportunistic bacteria called non-tuberculous mycobacteria (NTM). While the incidence of tuberculosis is declining in the developed world, infection rates by NTM are increasing. NTM
Bharat P Gurale et al.
The Journal of organic chemistry, 77(13), 5801-5807 (2012-06-06)
Concise and efficient syntheses of the aminocyclitol cores of hygromycin A (HMA) and methoxyhygromycin (MHM) have been achieved starting from readily available myo-inositol. Reductive cleavage of myo-inositol orthoformate to the corresponding 1,3-acetal, stereospecific introduction of the amino group via the
Bernd Becker et al.
ACS chemical biology, 8(1), 105-115 (2012-11-01)
Aminoglycoside antibiotics were among the first antibiotics discovered and used clinically. Although they have never completely fallen out of favor, their importance has waned due to the emergence of other broad-spectrum antibiotics with fewer side effects. Today, with the dramatically
Shijie He et al.
PloS one, 14(2), e0202065-e0202065 (2019-02-02)
Loss of function of the tumor suppressor p53 is known to increase the rate of migration of cells transiting the narrow pores of the traditional Boyden chamber assay. Here by contrast we investigate how p53 impacts the rate of cellular
Michael J McDonald et al.
Current biology : CB, 22(13), 1235-1240 (2012-06-26)
Mutation is the source of both beneficial adaptive variation and deleterious genetic load, fueling the opposing selective forces than shape mutation rate evolution. This dichotomy is well illustrated by the evolution of the mutator phenotype, a genome-wide 10- to 100-fold
Rengasamy Ramamoorthy et al.
Plant cell reports, 31(10), 1923-1931 (2012-06-27)
The increasing interest in renewable energy has attracted more research attention on biofuels. In order to generate sustainable amount of biomass feedstock from dedicated biofuel crops such as switchgrass they need to be genetically improved. Genetic transformation is one of
Jung-Won Lee et al.
Nature communications, 10(1), 1897-1897 (2019-04-25)
The cellular decision regarding whether to undergo proliferation or death is made at the restriction (R)-point, which is disrupted in nearly all tumors. The identity of the molecular mechanisms that govern the R-point decision is one of the fundamental issues
Daniela Cornacchia et al.
The EMBO journal, 31(18), 3678-3690 (2012-08-02)
The eukaryotic genome is replicated according to a specific spatio-temporal programme. However, little is known about both its molecular control and biological significance. Here, we identify mouse Rif1 as a key player in the regulation of DNA replication timing. We
Iksung Jin et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(23), 9137-9142 (2012-05-24)
Whereas short-term (minutes) facilitation at Aplysia sensory-motor neuron synapses is presynaptic, long-term (days) facilitation involves synaptic growth, which requires both presynaptic and postsynaptic mechanisms. How are the postsynaptic mechanisms recruited, and when does that process begin? We have been investigating
Sanjeev Kumar et al.
PloS one, 7(11), e48982-e48982 (2012-11-08)
Chronological aging of yeast cells is commonly used as a model for aging of human post-mitotic cells. The yeast Saccharomyces cerevisiae grown on glucose in the presence of ammonium sulphate is mainly used in yeast aging research. We have analyzed
Chun-Yu Zhang et al.
Yi chuan = Hereditas, 34(12), 1599-1606 (2012-12-25)
The decision of using selection marker is one of the key factors for success of plant genetic transformation and offspring screening. As two commonly used selection markers, hpt and bar genes are widely used in tissue culture-based rice transformation. To
Mauricio Niklitschek et al.
Methods in molecular biology (Clifton, N.J.), 898, 219-234 (2012-06-20)
Generally two selection markers are required to obtain homozygous mutations in a diploid background, one for each gene copy that is interrupted. In this chapter is described a method that allows the double gene deletions of the two copies of
Akane Kubota et al.
Bioscience, biotechnology, and biochemistry, 77(1), 167-172 (2013-01-08)
The thallus, the gametophyte body of the liverwort Marchantia polymorpha, develops clonal progenies called gemmae that are useful in the isolation and propagation of isogenic plants. Developmental timing is critical to Agrobacterium-mediated transformation, and high transformation efficiency has been achieved
Hyojin Kim et al.
PloS one, 8(2), e56476-e56476 (2013-02-27)
The ability to enrich cells with targeted mutations greatly facilitates the process of using engineered nucleases, including zinc-finger nucleases and transcription activator-like effector nucleases, to construct such cells. We previously used surrogate reporters to enrich cells containing nuclease-induced mutations via
Sabine Gruber et al.
BMC research notes, 5, 641-641 (2012-11-20)
Species of the fungal genus Trichoderma are important industrial producers of cellulases and hemicellulases, but also widely used as biocontrol agents (BCAs) in agriculture. In the latter function Trichoderma species stimulate plant growth, induce plant defense and directly antagonize plant
Yuko Konishi et al.
BioTechniques, 53(3), 141-152 (2012-09-12)
Gene targeting in a broad range of human somatic cell lines has been hampered by inefficient homologous recombination. To improve this technology and facilitate its widespread application, it is critical to first have a robust and efficient research system for
Katharina Viola et al.
Archives of toxicology, 87(7), 1301-1312 (2013-03-19)
Health beneficial effects of xanthohumol have been reported, and basic research provided evidence for anti-cancer effects. Furthermore, xanthohumol was shown to inhibit the migration of endothelial cells. Therefore, this study investigated the anti-metastatic potential of xanthohumol. MCF-7 breast cancer spheroids
Andrew K Hastings et al.
Journal of virology, 93(13) (2019-04-12)
Saliva from the mosquito vector of flaviviruses is capable of changing the local immune environment, leading to an increase in flavivirus-susceptible cells at the infected bite site. In addition, an antibody response to specific salivary gland (SG) components changes the
Ayaz Najafov et al.
Molecular cell, 75(3), 457-468 (2019-06-25)
Necroptosis, a cell death pathway mediated by the RIPK1-RIPK3-MLKL signaling cascade downstream of tumor necrosis factor α (TNF-α), has been implicated in many inflammatory diseases. Members of the TAM (Tyro3, Axl, and Mer) family of receptor tyrosine kinases are known for
Goetz Hensel et al.
BMC plant biology, 12, 171-171 (2012-09-26)
While the genetic transformation of the major cereal crops has become relatively routine, to date only a few reports were published on transgenic triticale, and robust data on T-DNA integration and segregation have not been available in this species. Here
Masayuki Nakamura et al.
Current microbiology, 65(2), 176-182 (2012-05-16)
Agrobacterium tumefaciens-mediated transformation (AtMT) has become a common technique for DNA transformation of yeast and filamentous fungi. In this study, we first established a protocol of AtMT for the phytopathogenic fungus Colletotrichum sansevieriae. Binary T-DNA vector containing the hygromycin B
M Cruz Muñoz-Centeno et al.
FEMS yeast research, 12(5), 511-520 (2012-03-16)
Using a yeast two-hybrid screen, we isolated a gene from Schizosaccharomyces pombe, whose product interacts with Mpg1, a GDP-mannose-1-phosphate guanylyltransferase involved in the maintenance of cell wall integrity and glycosylation. We have designated this gene mpg2 based on its similarity
Paul Bourgine et al.
Stem cell research, 12(2), 584-598 (2014-02-25)
The hTERT-immortalization of human bone marrow-derived Mesenchymal Stromal Cells (hMSCs) was proposed to address availability/standardization issues for experimental or clinical studies, but raised concerns due to possible uncontrolled growth or malignant cell transformation. Here we report a method to generate
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