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Conformations of a metastable SH3 domain characterized by smFRET and an excluded-volume polymer model.
Mazouchi A, et al.
Biophysical Journal, 110(7), 1510-1522 (2016)
Sanjiv K Mishra et al.
Protein expression and purification, 80(2), 234-238 (2011-07-13)
C-phycoerythrin was isolated and purified from marine Pseudanabaena sp. using two step chromatographic methods. Phycobiliproteins in the marine Pseudanabaena was extracted in 100 mM phosphate buffer (pH 7.2) and precipitated by salting out. The precipitated C-phycoerythrin was purified by gel
Fernanda O Lemos et al.
European journal of dermatology : EJD, 21(5), 722-730 (2011-07-09)
Previous studies demonstrated that proteinases from latex of C. candamarcensis act as mitogens on fibroblast and epithelial cells and a subsequent report showed their protective, angiogenic and wound healing effects on gastric ulcers. In this study, we present evidence of
Li-Ming Shen et al.
Talanta, 85(3), 1285-1290 (2011-08-03)
A flow-through fluorescence optosensor with Sephadex G-50 microbeads as solid support is developed for the sensitive determination of tetracycline (TC). The fluorescent TC derivative encapsulated in CTAB micelle structures is retained onto the surface of the microbeads packed into a
Shuoye Yang et al.
International journal of pharmaceutics, 434(1-2), 155-160 (2012-05-31)
Three most commonly used preparative methods, dry-film, reverse phase evaporation and ethanol injection were employed to prepare cationic liposomes composed of DC-Chol and DOPE, respectively. The resulting samples were contrasted through morphology observation, particle size and zeta potential analysis. Sephadex
Sandipan Datta et al.
Journal of natural products, 76(4), 642-647 (2013-02-28)
Natural product drug discovery programs often rely on the use of silica (Si) gel, reversed-phase media, or size-exclusion resins (e.g., RP-C18, Sephadex LH-20) for compound purification. The synthetic polymer-based sorbent Diaion HP20SS (cross-linked polystyrene matrix) is used as an alternative
Yosra Ayed et al.
Annals of clinical microbiology and antimicrobials, 11, 15-15 (2012-06-14)
Toxins derived from jellyfishes have been exploited as a model for the development of new drug promising applications to treat neurodegenerative diseases. The present work is aimed to evaluate the acute toxicity of crude venom of Pelagia noctiluca and then
Jianrong Li et al.
Journal of food science, 77(6), C640-C645 (2012-05-18)
γ-Glutamyl-transpeptidase (GGT) is one of the important enzymes in the pathway of odor formation in shiitake mushroom (Lentinus edodes). Rapid purification and characterization of GGT from shiitake mushroom were studied in this work. The GGT was purified 179-fold after 3
Helena Siller et al.
Pulmonary pharmacology & therapeutics, 25(3), 223-227 (2012-03-27)
Pulmonary inflammation disorders represent a major healthcare burden, and novel anti-inflammatory agents are critically needed for the treatment of patients unresponsive to current therapies. In vivo animal models play a key role in the preclinical assessment of novel anti-inflammatory compounds. The implementation
Aline Mendes Maziero et al.
Life sciences, 93(9-11), 416-422 (2013-07-23)
The relationship between the activity of eosinophils and platelets has been observed in recent decades by many scientists. These observations include increased numbers of eosinophils associated with platelet disorders, including changes in the coagulation cascade and platelet aggregation. Based on
Jana Schwarzer et al.
Electrophoresis, 29(20), 4203-4214 (2008-10-18)
Glycoproteins, such as monoclonal antibodies as well as recombinant and viral proteins produced in mammalian cell culture play an important role in manufacturing of many biopharmaceuticals. To ensure consisting quality of the corresponding products, glycosylation profiles have to be tightly
M O Goodarzi et al.
Human reproduction (Oxford, England), 23(12), 2842-2849 (2008-09-02)
Human homologs (FEM1A, FEM1B, FEM1C) of nematode sex determination genes are candidate genes for polycystic ovary syndrome (PCOS). We previously identified a FEM1A mutation (H500Y) in a woman with PCOS; FEM1B has been implicated in insulin secretion. Women with and
Yen-Hui Lin
Water science and technology : a journal of the International Association on Water Pollution Research, 66(12), 2604-2612 (2012-10-31)
Molecular weight (MW) distribution of organic matter by ozonation and biofiltration was evaluated using gel chromatography. The MW distribution of organic matter by Sephadex G-25 was observed from groups 2 (MW = 1,029-7,031 g/mol) and 3 (MW = 303-1,029 g/mol)
Qiang Luo et al.
Journal of medicinal food, 14(12), 1609-1616 (2011-09-01)
Crude water-soluble Chinese truffle Tuber indicum polysaccharide (TIP) was extracted from the fruiting bodies with water and then successively purified by DEAE-cellulose 52 and Sephadex G-100 column chromatography, yielding two major polysaccharide fractions: TIP1-1 and TIP2-1. High-performance gel permeation chromatography
Qing-Qing Wang et al.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica, 38(3), 366-370 (2013-05-15)
To study the chemical constituents of Rhododendron seniavinii. Compounds were isolated from the aqueous extract of the leaves of R. seniavinii by using Sephadex LH-20, ODS open column chromatography and other means. Their structures were elucidated according to spectral data
Xing-Bo Li et al.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica, 38(10), 1543-1547 (2013-08-21)
Sixteen compounds were isolated from Conioselinum vaginatum by silica column chromatography over silica gel and Sephadex LH-20, as well as recrystallization. On the basis of their physical and chemical properties and spectral data, their structures were identified as ligustilide (1)
Lei Wang et al.
International journal of nanomedicine, 6, 2641-2652 (2011-11-25)
Single-walled carbon nanotubes (SWNT) are poorly soluble in water, so their applications are limited. Therefore, aqueous solutions of SWNT, designed by noncovalent functionalization and without toxicity, are required for biomedical applications. In this study, we conjugated docetaxel with SWNT via
Nerine T Joseph et al.
The Journal of endocrinology, 202(1), 179-190 (2009-04-22)
Two GnRH isoforms (cGnRH-I and GnRH-II) and two GnRH receptor subtypes (cGnRH-R-I and cGnRH-R-III) occur in chickens. Differential roles for these molecules in regulating gonadotrophin secretion or other functions are unclear. To investigate this we cloned cGnRH-R-III from a broiler
Xiao-Yu Tao et al.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica, 37(24), 3738-3742 (2013-05-01)
The column chromatography on silica gel, Sephadex LH-20 and semi-preparative HPLC were used to separate and purify the compounds from the EtOAc extract of medium and MeOH extract of cell cultures of Morus alba. Eight compounds were isolated. Based on
Ebru Akkemik et al.
Environmental toxicology and pharmacology, 34(3), 888-894 (2012-09-20)
The glutathione S-transferases (EC were purified and characterized from turkey liver for the first time. The enzyme was purified 252.7-fold with a yield of 45%, with a specific activity of 164.31 U/mg from turkey liver. The purity of the
Jun-Feng Gao et al.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica, 38(10), 1556-1559 (2013-08-21)
Chemical constituents were investigated on the ethyl acetate extract of Salvia chinensis. Compounds were separated and purified by various chromatograhic techniques including silica gel, Sephadex LH-20 and reversed-phase HPLC. Their structures were identified by spectroscopic data analysis. Eleven compounds were
Yong Ding et al.
Journal of chromatography. A, 1218(47), 8503-8510 (2011-10-26)
Refolding enables bioprocesses predicated on proteins expressed as inclusion bodies in Escherichia coli. Optimization of size-exclusion chromatography (SEC) refolding is a significant challenge because a wide range of factors, including the choice of gel media, the column dimensions and configuration
Ritu Dixit et al.
Human mutation, 39(12), 1957-1972 (2018-08-29)
Transcription factor GATA4 is known to play crucial role during heart development, regulating expression of several other key cardiogenic factors. Various GATA4 mutations are reported in familial as well as sporadic cases of congenital heart defects (CHDs). To estimate the
Anna Gerdova et al.
Chirality, 23(7), 574-579 (2011-06-29)
There are well established theoretical models for correction for absorption flattening of circular dichroism (CD) measurements on particle suspensions. However, these have not been directly tested experimentally. We describe a test system with the chiral tris(ethylenediamine)Co(III) complex dissolved in water
Zhipeng Li et al.
International immunopharmacology, 14(2), 145-149 (2012-07-10)
The study is to investigate the effect of compound IMMLG5521, a coumarin derivative, on lung inflammation induced by Sephadex in rats. Sephadex led to massive granulomas, infiltration of neutrophils and eosinophils, the increase of TNF-α level in BALF and lung
Anna Rethy et al.
International journal of computer assisted radiology and surgery, 13(1), 61-72 (2017-09-21)
The objective of this study was to develop a multimodal, permanent liver phantom displaying functional vasculature and common pathologies, for teaching, training and equipment development in laparoscopic ultrasound and navigation. Molten wax was injected simultaneously into the portal and hepatic
Marcílio S S Cunha-Filho et al.
The Journal of pharmacy and pharmacology, 63(9), 1156-1160 (2011-08-11)
The purpose of this work was to study the chemical stability of the new antitumoral β-lapachone (βLAP) to determine the degradation pathway/s of the molecule and the degradation kinetics in addition to identifying several degradation products. Samples of βLAP in
Lila Boulekbache-Makhlouf et al.
Phytochemical analysis : PCA, 24(2), 162-170 (2012-08-30)
Eucalyptus species are widely cultivated in Mediterranean regions. Moreover, plants of this family have been utilized for medicinal purposes. A number of studies have been devoted to the identification of eucalypt phenolics, all of them have focused on specific families
Olga N Solovjeva et al.
The protein journal, 31(2), 137-140 (2012-01-03)
Catalytic activity has been demonstrated for holotransketolase in the absence of free bivalent cations in the medium. The two active centers of the enzyme are equivalent in both the catalytic activity and the affinity for the substrates. In the presence
P M G Paiva et al.
Bioresource technology, 88(1), 75-79 (2003-02-08)
A highly purified trypsin inhibitor was obtained from Echinodorus paniculatus when an extract prepared from E. paniculatus seed flour (25 gl(-1), with 0.1 M ammonium acetate buffer, pH 8.3, under agitation for 6 min at 28 degrees C) was chromatographed
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