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Fetal Bovine Serum Basics
A lab commodity whose quality may often be taken for granted, serum can make—or break—the outcome of all of your cell-based assays. These 5 key subjects will provide you with a strong educational foundation in the use of sera for
Fetal Bovine Serum (FBS)
Fetal bovine serum (FBS), sometimes called fetal calf serum (FCS), is a supplement for mammalian cell culture that is rich in embryonic growth-promoting factors. Choose FBS grade based on requirements and application specificity.
Bovine Spongiform Encephalopathy (BSE)-free fetal bovine serum (FBS)
Ensure that bovine products for research and production are free of bovine spongiform encephalopathy. Find BSE-free products including fetal bovine serum (FBS).
Serum Cross Reference Tables
Serum cross reference tables allow you to find our products to replace sera from Invitrogen/Gibco, Hycolne, Mediatech, Biowhittaker, Biomeda, and Fisher.
Sera
Origin-certified, quality-controlled cell culture sera include FBS, other bovine sera, human serum, and sera from diverse animal species, rigorously screened for contaminants and performance-tested in culture.
Choosing the Optimal Cell Culture Media for Vaccine Production
Choosing the optimal cell culture media for vaccine production
Choosing the optimal cell culture media for vaccine production
Choosing the optimal cell culture media for vaccine production
Media Cross Reference (Europe)
Invitrogen/Gibco Media Cross Reference Chart for tissue culture media. Find direct replacements for Gibco cell culture products in Europe.
Choosing the optimal cell culture media for vaccine production
Cultured cells are excellent hosts for the propagation of many types of viruses. The ability of cell culture systems to produce large quantities of attenuated viral particles has served as the basis for the production of both human and veterinary
(Other) Sera
Although FBS is the most common serum variety used for cell culture, adult and newborn bovine serum and sera from other species are alternatives for cell culture supplementation.
9CFR AVA Standards for Adventitious Virus Testing of Bovine Sera
9CFR (Code of Federal Regulations) testing of bovine sera for adventitious viral agents (AVA). Methods include cytopathic effects, hemadsorption assay, and antibody detection.
Cell Culture Media Preparation
Culture media preparation for in vitro cell cultivation including preparation of different media formats, sterilization methods, cell growth and regulation supplements, media storage and use.
Human Epidermal Keratinocytes (HEK) Culture Protocol
Storage - Preparation for Culturing - Culturing HEK - Subculturing HEK
Protocol for Charcoal-stripping FBS to Deplete Hormones
Protocol for charcoal stripping fetal bovine serum for hormonal depletion, with and without heat inactivation.
Microbe selection for the SER-TAIN™ gamma irradiation FBS validation process
Learn how relevant microbe selection for process validation of the SER-TAIN™ gamma irradiation of bovine serum ensures safer serum for cell culture.
Cell Freezing Reagents
Protect valuable cell line stocks by choosing reagents that are designed to protect cells during ultralow temperature storage. Our DMSO selection is application-tested, and ready-to-use cell freezing media are available in DMSO-free and in variable DMSO concentrations.
Pfizer Compounds
Pfizer research compounds in support of your neuroscience, gene regulation, ADME/Tox, immunology, ion channels and lipid signaling, apoptosis and cell cycle, cytokines and growth factor drug discovery workflows.
Serum free cancer cell line media
Defined culture conditions. Consistent growth of human cancer cell lines in PromoCell’s serum-free/xeno-free Cancer Cell Line Media-XF. Explore over 350 PromoCell products on Sigma.com
Structural Genomics Consortium
Structural Genomics Consortium (SGC) chemical probes for target validation and numerous additional research applications and workflow needs.
Frequently-asked questions about gamma-irradiated FBS (serum) and other products for cell culture
Find answers to questions (FAQs) about fetal bovine serum (FBS) that is gamma-irradiated by the SER-TAIN™ process. Request a sample of FBS at Sigma-Aldrich.com.
Structural Genomics Consortium
Structural Genomics Consortium (SGC) chemical probes for target validation and numerous additional research applications and workflow needs.
Pfizer® Compounds
Pfizer research compounds in support of your neuroscience, gene regulation, ADME/Tox, immunology, ion channels and lipid signaling, apoptosis and cell cycle, cytokines and growth factor drug discovery workflows.
Media and Supplements in Cell Culture
An overview of basic cell culture media and supplements including fetal bovine serum, basal media, salts, antibiotics, proteins and amino-acids. Free ECACC handbook download.
Primary Cell Culture
Primary cells isolated directly from human and animal sources maintain physiological relevance of the tissues from which they are derived, and are used for ADME/Tox screening. Find applications and methods for culturing mammalian primary cells in vitro.
Corning Cellgro Culture Cross-Reference Chart
This is a cell culture cross-reference chart to find our cell culture products, inclusing Corning Cellgro's cell culture products and DPBS.
PLTMax® Human Platelet Lysate (hPL): A Superior Serum-Free Media Supplement for Robust Mesenchymal Stem Cell Expansion
PLTMax® Human Platelet Lysate (hPL) is a superior serum-free and xeno-free media supplement alternative to fetal bovine serum (FBS) for human mesenchymal stem cell (MSC) cultures.
Serum for Cell and Tissue Culture FAQs
Find answers to frequently asked questions (FAQs) about FBS and other serum products, and links to related products.
Human Serum
Human serum is used to culture human cells and is available as plasma-derived AB human serum, clotted whole blood AB human serum, heat inactivated preparations, and complement-depleted preparations.
Sterile Laboratory Filtration Devices
Prepare cell culture media and filter-sterilize aqueous solutions from a few mL to 20 L using industry-leading Millipore® sterile filtration devices. Choose fit-for-application membranes and pore sizes in Steriflip®, Sterivex®, Stericap®, and Steripak® devices.
Stem Cells
Our complete range of ES and iPS cell-qualified products includes stem cell lines and media for differentiation and maturation, and provides stem cell researchers with convenient and cost-effective solutions for the reliable culture of pluripotent stem cells.
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