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Fetal Bovine Serum

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Fetal Bovine Serum Basics
A lab commodity whose quality may often be taken for granted, serum can make—or break—the outcome of all of your cell-based assays. These 5 key subjects will provide you with a strong educational foundation in the use of sera for
Bovine Spongiform Encephalopathy (BSE)-free fetal bovine serum (FBS)
Ensure that bovine products for research and production are free of bovine spongiform encephalopathy. Find BSE-free products including fetal bovine serum (FBS).
Choosing the optimal cell culture media for vaccine production
Choosing the optimal cell culture media for vaccine production
Choosing the optimal cell culture media for vaccine production
Cultured cells are excellent hosts for the propagation of many types of viruses. The ability of cell culture systems to produce large quantities of attenuated viral particles has served as the basis for the production of both human and veterinary
Choosing the optimal cell culture media for vaccine production
Choosing the optimal cell culture media for vaccine production
9CFR AVA Standards for Adventitious Virus Testing of Bovine Sera
9CFR (Code of Federal Regulations) testing of bovine sera for adventitious viral agents (AVA). Methods include cytopathic effects, hemadsorption assay, and antibody detection.
PLTMax® Human Platelet Lysate (hPL): A Superior Serum-Free Media Supplement for Robust Mesenchymal Stem Cell Expansion
PLTMax® Human Platelet Lysate (hPL) is a superior serum-free and xeno-free media supplement alternative to fetal bovine serum (FBS) for human mesenchymal stem cell (MSC) cultures.
Media and Supplements in Cell Culture
An overview of basic cell culture media and supplements including fetal bovine serum, basal media, salts, antibiotics, proteins and amino-acids. Free ECACC handbook download.
Microbe selection for the SER-TAIN™ gamma irradiation FBS validation process
Learn how relevant microbe selection for process validation of the SER-TAIN™ gamma irradiation of bovine serum ensures safer serum for cell culture.
Frequently-asked questions about gamma-irradiated FBS (serum) and other products for cell culture
Find answers to questions (FAQs) about fetal bovine serum (FBS) that is gamma-irradiated by the SER-TAIN™ process. Request a sample of FBS at Sigma-Aldrich.com.
Serum for Cell and Tissue Culture FAQs
Find answers to frequently asked questions (FAQs) about FBS and other serum products, and links to related products.
Quality Control Considerations in Cell Culture
Quality control guidelines to maintain high quality authenticated and contamination-free cell cultures. Free ECACC handbook download.
Corning® U-shaped 150 and 175 cm2 Flasks Demonstrate Same Cell Growth as Traditional Rectangular Design
Corning® U-shaped 150 and 175 cm2 Flasks Demonstrate Same Cell Growth as Traditional Rectangular Design
Primary Human Fibroblasts and Growth Media
Primary human fibroblasts and growth medium for the in vitro cultivation of juvenile and adult fibroblasts. Protocols for handling, cell passaging, media, and product use.
Mesenchymal Stem Cell FAQs
Frequently asked questions about mesenchymal stem cells including MSC derivation, expansion, differentiation and allogenic stem cell therapy.
Mycoplasma Detection and Elimination
Detect mycoplasma contamination in cell cultures three ways: culture test, DNA stain test, or mycoplasma PCR test. Mycoplasma elimination kits rid cultures of contamination.
Rapid Analysis of Human Adipose-Derived Stem Cells and 3T3-L1 Differentiation Towards Adipocytes Using the Scepter™ 2.0 Cell Counter
Scepter cell counter is a tool to track phenotypic change in differentiation of human adipose-derived stem cells towards adipocytes.
Primary Human Osteoblasts and Osteoblast Growth/Differentiation Media
Primary human osteoblasts and media for the optimal growth and mineralization of human osteoblast cultures. Protocols for handling, cell passaging, media, and product use.
Primary Human Follicle Dermal Papilla Cells and Growth Media
Primary human follicle dermal papilla cells and optimized culture medium. Protocols for handling, cell passaging, media, and product use.
Detection of Endogenous Pathway Activity in Novel Reporter Cell Lines
Our company presents an article on the Detection of Endogenous Pathway Activity in Novel Reporter Cell Lines
Cell Culture FAQs: Bacterial Endotoxin Contamination
What is endotoxin? Frequently asked questions about bacterial endotoxin contamination of in vitro cell cultures. Details about how to endotoxin test using the LAL assay, common sources of laboratory endotoxin contamination and tips on how to avoid endotoxin contamination when
Primary Human Skeletal Muscle Cells and Growth Media
Primary human skeletal muscle cells and culture medium optimized for the in vitro cultivation and differentiation of primary human skeletal muscle cells. Protocols for handling, cell passaging, media, and product use.
3D Neurosphere Culture Media
3D neurosphere culture protocol to expand human U-87 MG glioblastoma cells in serum-free/xeno-free stem cell culture media. Explore over 350 PromoCell products on Sigma.com.
Cancer Stem Cell Proliferation in Human Prostate and Breast Cancer Cell Lines Utilizing a New Defined Serum-Free 3D Spheroid Media
Serum-free defined cancer stem cell media used to grow and expand cancer cells in 3D tumorsphere aggregates.
Primary Human Smooth Muscle Cells and Growth Media
Smooth muscle human primary cells isolated from blood vessels and hollow organs, plus smooth muscle cell growth media. Protocols on media handling, cell passaging and product use.
Primary Human Endothelial Cells and Growth Media
Primary human endothelial cells isolated from microvascular tissue and large blood vessels including HUVEC plus optimized growth media. Protocols for handling, cell passaging, media, and product use.
Technical Bulletins
Technical bulletins for our shRNA products, including Bacterial Glycerol Stock, Purified Plasmid DNA Format, Lentiviral Format, MISSION® Control Vectors, MISSION Control Transduction Particles, MISSION Lentiviral Packaging Mix.
Antibody Basics
Technical Support in Antibody Basics includes basic structure, classes and normal immunoglobulin ranges.
Cell Culture Contamination Troubleshooting
Common Cell Culture Problems: Contamination is easily the most common problem encountered in cell culture laboratories, sometimes with very serious consequence.
Attachment-Dependent Cell Growth and Cell Proliferation in the MultiScreen Assay System
This article discusses attachment-dependent cell growth and cell proliferation investigations using the MultiScreen Assay System from Millipore.
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