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FACS Titration of Lentivirus Protocol
FACS (Fluorescence-Activated Cell Sorting) provides a method for sorting a mixed population of cells into two or more groups, one cell at a time, based on the specific light scattering and fluorescence of each cell. This method provides fast, objective
Lentiviral Production Using X-tremeGENE HP Transfection Reagent
Lentiviruses represent a powerful tool in research applications to transduce a wide range of cell types.
Lentiviral Transduction Protocol
Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.
X-tremeGENE™ 9 DNA Transfection Reagent Protocol
Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).
Co-transfection of Plasmid DNA
Transient co-transfection of plasmids is a method that is commonly employed for cellular protein-protein interaction studies, transcription factor studies, and gene knockdown studies using shRNA encoding plasmids.
Universal Transfection Reagent Protocol
Our Universal Transfection Reagent is a unique formulation of a proprietary polymer blend used for transient and stable transfection of nucleic acids into various eukaryotic cell lines and hard-to-transfect primary cells. This is a fast and easy protocol is compatible
Protocol for Transducing Mouse Embryonic Fibroblasts (MEF) using MISSION® ExpressMag™ Super Magnetic Kit®
This detailed procedure allows you to transduce Mouse Embryonic Fibroblasts (MEF) using MISSION ExpressMag Super Magnetic Kit.
Liposome Preparation - Avanti® Polar Lipids
Properties of lipid formulations can vary depending on the composition (cationic, anionic, neutral lipid species), however, the same preparation method can be used for all lipid vesicles regardless of composition. The general elements of the procedure involve preparation of the
X-tremeGENE™ HP DNA Transfection Reagent Protocol
Cell preparation for transfection Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).
Preparation of Cationic Liposomes & Transfection of Cells - Avanti® Polar Lipids
This procedure describes the Preparation of Cationic Liposomes & Transfection of Cells
Protocols for Transfecting Common Cell Lines with X-tremeGENE™ Transfection Reagents
Protocols for Transfecting Common Cell Lines with X-tremeGENE™ Transfection Reagents
Simplicon™ RNA Transfection and Electroporation Protocols
Simplicon™ RNA Transfection and Electroporation Protocols
Calcium Phosphate Transfection Kit Protocol
Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.
Preparation of Competent Cells and Transformation with pGEX DNA
This page describes preparation of competent cells and transformation with pGEX DNA.
Yeast Transformation Kit
The selection of plasmids in yeast is based on the use of auxotrophic mutant strains, which cannot grow without a specific medium component (an amino acid, purine, or pyrimidine)
Escort™ IV Transfection Reagent Protocol
Product manual provides detailed protocol for easy DNA transfection.
X-tremeGENE™ siRNA Transfection Reagent Protocol & Troubleshooting
X-tremeGENE™ siRNA Transfection Reagent Protocol & Troubleshooting
Genome Editing in Plants with CRISPR/Cas9
Zinc finger nucleases (ZFNs), were introduced by Sigma-Aldrich less than 8 years ago, but in that time the technology of targeted genome editing has advanced rapidly. Most recently, the discovery of the CRISPR/Cas9 pathway has accelerated interest in this field
CRISPR Lentiviral Screening: Antibiotic Selection
You are not alone designing successful CRISPR, RNAi, and ORF experiments. Sigma-Aldrich was the first company to commercially offer lentivirus versions of targeted genome modification technologies and has the expertise and commitment to support new generations of scientists.
Escort™ III Transfection Reagent Protocol
The product bulletin providin detailed use protocol for easy DNA transfection.
Lipid Transfection Optimization Protocol
Expert guidance on the best way to optimize your transfection experiments. Optimized protocols result in highest efficiency transfections.
Procedure for Preparation of Cationic Lipid/Oligomer Complex - Avanti® Polar Lipids
This procedure describes the preparation of Cationic Lipid/Oligomer Complex.