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Purification of Membrane Proteins
This page shows how to perform a purification of His-tagged membrane proteins.
Performing a Purification of IgM Antibodies with HiTrap™ IgM Purification HP
This page shows how to purify IgM antibodies by affinity chromatography using HiTrap™ IgM Purification HP.
Optimizing Purification of Histidine-Tagged Proteins
How to optimize purification of histidine-tagged proteins using Cytiva products.
Salt Selection and Buffer Preparation
The correct choice of salt and salt concentration are the most important parameters that influence capacity and final selectivity. The objective is to optimize conditions to achieve the required selectivity to bind the target protein(s) and ensure that the majority
Principles and Standard Conditions for Different Purification Techniques
This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using Cytiva products.
ANTI-FLAG® M2 Magnetic Beads
The FLAG® Expression System is a proven method to express, purify and detect recombinant fusion proteins. Sigma®, the proven provider of FLAG®, now offers a magnetic bead for immunoprecipitation, protein purification, and the study of protein-protein interactions. The ANTI-FLAG® M2
Column, Media and Sample Preparation for Hydrophobic Interaction Chomatography
Here the correct ways for Column, Media and Sample preparation for Hydrophobic Interaction Chomatography (HIC) with Cytiva media are detailed, including sample application, load volume and temperature
Purification of Thiol-Containing Substances By Covalent Chromatography
Thiol-containing substances can be isolated selectively by covalent binding to an activated thiolated matrix via thiol-disulphide exchange to form a mixed disulphide bond. After washing away unbound material, the thiol-containing substance is eluted by reducing the disulphide bond. This technique
Purification or Removal of Viruses including Adeno-associated Virus
This page shows how to purify or remove viruses with a Capto DeVirS, AVB Sepharose High Performance from Cytiva.
Troubleshooting of Problems Associated with the Expression and Growth of GST-tagged Proteins
This page addresses common problems associated with the expression and growth of GST-tagged proteins using Cytiva products.
Peptide Impurities
Peptide Impurities
Troubleshooting of Problems Associated with the Expression and Growth of GST-tagged Proteins
This page addresses common problems associated with the expression and growth of GST-tagged proteins using GE Healthcare products.
Deglycosylation Kits
Deglycosylation Kits
Troubleshooting Strategies in GST-tagged Protein Purification
This page describes troubleshooting strategies in purification of GST-tagged proteins using Cytiva products.
Affinity Chromatography Troubleshooting
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
General Considerations for Purification of GST-tagged Protein
The yield of GST-tagged proteins is highly variable, ranging from 1 mg/l to 10 mg/l. The yield depends on various parameters, such as nature of the tagged protein, the host cell, and the culture conditions used.
Column Packing and Preparation for Affinity Chromatography of Antibodies
This page describes efficient column packing and preparation for affinity chromatography of antibodies.
Amicon® Pro Purification System – Protein Purification
Protein purification is simpler with Amicon Pro. The device delivers consistent sample preparation for more reliable recovery, uncompromised purity and easier data generation.
Troubleshooting of Cleavage Methods
This page shows troubleshooting strategies for cleavage methods using Cytiva products.
Protein G and Protein A Bind to Different IgG
This page shows a comparison of the relative binding strengths of protein G and protein A to different immunoglobulins.
Purification or Removal of Proteins and Peptides
This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from Cytiva.
Converting from Flow Velocity to Volumetric Flow Rates
This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.
Agarose Beads Vs. Magnetic Beads in ChIP
Agarose beads Vs. Magnetic beads in Chromatin Immunoprecipitation (ChIP)
Characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (High Sub) Media and Columns
This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.
Purification Using Protein A-based Chromatography Media
This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.
Polishing of MAbs Using Capto Adhere ImpRes in Bind/Elute Mode
In these studies, the binding capacity for MAbs and the efficiency in the clearance of impurities using Capto adhere ImpRes in bind/elute mode was evaluated.
Cleavage and Purification of GST-Tagged Protein Eluted from GSTrap
This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from Cytiva.
Performing an Isolation of Recombinant Protein Complexes
This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from Cytiva.
Purification using GST SpinTrap™
This page shows how to purify GST-tagged proteins using GST SpinTrap™ from Cytiva.
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