Applications
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Column Packing and Preparation for Hydrophobic Interaction and Reversed Phased Chromatography
This page discusses column packing and preparation techniques for reverse phase chromatography
Manual Purification of Histidine-Tagged Proteins using His GraviTrap™ TALON®
This page describes manual purification of histidine-tagged proteins using His GraviTrap TALON from GE Healthcare.
General Considerations for Purification of GST-tagged Proteins
This page describes general considerations for purification of GST-tagged proteins using GE Healthcare products.
Troubleshooting Purification Methods
This page shows troubleshooting methods for purification of GST-tagged proteins using GE Healthcare products.
Organelle Isolation
The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate and purify...
Characteristics of Dextrin Sepharose® High Performance Products
This page shows the characteristics of Dextrin Sepharose High Performance products from GE Healthcare.
Capto Adhere
Capto adhere from GE Healthcare is a multimodal strong anion exchanger for BioProcess applications.
Performing a separation with HiTrap Protein G HP
This page shows how to perform a separation with the HiTrap Protein G HP from GE Healthcare, an Affinity Exclusion Chromatography product containing pre-packed Protein G.
Maintenance of Multimodal Chromatography Media and Storage Conditions
This page describes the maintenance of media and storage conditions for multimodal chromatography using GE Healthcare products.
Performing a purity and homogeneity check
This page shows how to perform a purification and homogeneity check of membrane proteins with products from GE Healthcare.
Troubleshooting Guide for Affinity Chromatography of Tagged Proteins
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.
Capto MMC
Capto MMC is a multimodal cation exchanger with the properties of a weak cation exchanger. In addition to electrostatic interactions, the ligand structure provides for additional interaction modes such as hydrophobic interaction, hydrogen bonding, and thiophilic interaction.
Troubleshooting of Detection Methods of GST-tagged Proteins
Troubleshooting of Detection Methods of GST-tagged Proteins
Performing a Separation with IgG Sepharose 6 Fast Flow
This page shows how to perform a separation with IgG Sepharose 6 Fast Flow from GE Healthcare, an Affinity Chromatography product for purification of recombinant fusion proteins containing a protein A tail.
HIC as a Capture Step
This page shows Hydrophobic Interaction Chromatography (HIC) in a purification strategy.
Performing High-Throughput Screening Using His MultiTrap™ HP and His MultiTrap™ FF 96-Well filter Plates
This page shows high-throughput screening using His MultiTrap HP and His MultiTrap FF 96-well filter plates from GE Healthcare.
Automated Purification of Antibodies Using ÄKTA Chromatography Systems
This page describes automated purification of antibodies using ÄKTA chromatography systems.
Performing a Purification of IgG Antibodies with Protein G GraviTrap™
This page shows how to separate IgG antibodies by affinity chromatography using Protein G GraviTrap from GE Healthcare.
Purifcation or Removal of Serine Proteases, e.g. Thrombin and Trypsin, and Zymogens
This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from GE Healthcare.
Principles and standard conditions for different purification techniques
This page provides information about the principles and standard conditions for different purification techniques for the purification of multiprotein complexes with from GE Healthcare.
Centrifugation-free Protein Extraction and Purification from Total Cultures of Baculovirus-infected Insect Cells
Centrifugation-free Protein Extraction and Purification from Total Cultures of Baculovirus-infected Insect Cells
Anti-FLAG® M2 Magnetic Beads
Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.
Protein Labeling and Purification with Amicon® Pro Centrifugal Filters
Commercially available protein labeling kits provide researchers with the flexibility to customize their own immunoassay detection panels.
Purification or Removal of Serine Proteases, such as Thrombin and Trypsin, and Zymogens
This page shows how to Purify or remove serine proteases with a Benzamidine Sepharose 4 Fast Flow, Benzamidine Sepharose 4 Fast Flow from GE Healthcare.
Cleavage and Purification of GST-Tagged Protein Bound to Glutathione Sepharose in Batch Mode
This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from GE Healthcare.
Performing a Purification of NADP+-dependent dehydrogenases and other enzymes with affinity for NADP+ Using 2’5’ ADP Sepharose 4B and Red Sepharose CL-6B
This page shows how to purify NADP+-dependent dehydrogenases and other enzymes with affinity for NADP+ by affinity chromatography using 2’5’ ADP Sepharose 4B and Red Sepharose CL-6B from GE Healthcare.
Automated IgG Purification Using PureProteom
PureProteome™ Protein A and G Magnetic beads provide a rapid and reproducible means to purify immunoglobulins (IgG) using the KingFisher Duo particle processor.
Crystallization
The elucidation of a macromolecular structure to the atomic level by X-ray or neutron diffraction analysis requires the molecule to be available in the form of relatively large single crystals. Many soluble proteins, membrane proteins, nucleic acids and nucleoprotein complexes...
Yeast Cell Lysis Reagents
Our yeast cell lysis reagents are versatile, phosphate-free, and non-denaturing. CelLytic Y Cell Lysis reagent provides an effective method for yeast cell lysis and protein solubilization. The CelLytic Y Yeast Enzymatic Lysis Kit provides a convenient method for...
Robust, load independent viral clearance in monoclonal antibody purification with NatriFlo® HD-Q membrane adsorber
Membrane adsorbers are a cost-effective, robust, single-use solution for monoclonal antibody polishing