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General Instructions for Affinity Purification Using HiTrap® Columns
General instructions for affinity purification using HiTrap columns from Cytiva.
Purification of Histidine-Tagged Recombinant Proteins Using Ni Sepharose® High Performance
Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions.
Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules
Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules
Sepharose® High Performance: Purification with High Resolution
Here we discuss purification options when working with Sepharose® High Performance and we present some examples of media screening, capture and purification.
Selectivity and the Properties of a HIC Medium
Selectivity of HIC medium from Cytiva is based on properties such as hydrophobicity, porosity and particle size.
Performing a Purification of Glycoproteins and Polysaccharides with Agarose Wheat Germ Lectin
This page shows how to purify glycoproteins and polysaccharides by affinity chromatography using Agarose Wheat Germ Lectin.
Performing a Separation of DNA binding proteins with Cytiva Products Based on Heparin
This page shows how to use heparin in the separation of DNA binding proteins used in HiTrap Heparin HP, HiPrep 16/10 Heparin FF and Heparin Sepharose 6 Fast Flow products from Cytiva.
Purification of Protein A-Tagged Proteins
This page shows how to purify protein A-tagged proteins using Cytiva products.
Removal of Thrombin and Factor Xa Using HiTrap® Benzamidine FF (High Sub)
To protect the fusion protein from proteolytic degradation prior to enzymatic cleavage with PreScission Protease, thrombin, or Factor Xa, it may be necessary to remove proteases from the sample. Additionally, following enzymatic cleavage, it may be necessary to remove thrombin
Performing a Separation with Superdex
Superdex from Cytiva are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.
Column Packing and Preparation
This page describes efficient column packing and preparation for affinity chromatography of tagged proteins using Cytiva products.
Removal of GST Tag by Enzymatic Cleavage
This page how to remove GST tags by enzymatic cleavage using Cytiva products.
Column Packing and Preparation for Size Exclusion Chromatography
Column Packing and Preparation for Size Exclusion Chromatography
Column Packing and Preparation Protocol for Affinity Chromatography
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.
Purification or Removal of Biotin and Biotinylated Biomolecules with Magnetic Beads
This page shows how to Purify or remove biotin and biotinylated biomolecules with magnetic beads.
Gravity-flow Purification using His GraviTrap™ and His GraviTrap™ Kit
This page shows how to purify histidine-tagged recombinant proteins by gravity flow using His GraviTrap and His GraviTrap Kit from Cytiva.
Purification from Unclarified Cell Lysate using HisTrap™ FF Crude
This page shows how to purify histidine-tagged recombinant proteins from unclarified cell lysate using HisTrap FF crude from Cytiva.
Capto Adhere ImpRes
Capto adhere ImpRes is a cost-effective and flexible chromatography medium from Cytiva, designed for high-resolution polishing of MAbs.
Antibody Purification using Protein A, Protein G, or Protein L Agarose
Antibody Purification using Protein A, Protein G, or Protein L Agarose protocol is designed as a quick purification method for antibodies from mammalian sera, ascites, and cell culture supernatants. It should be noted that if the starting material is serum
Performing a Separation with Cytiva Products Based on Poly (His) Fusion Proteins
This page shows how to perform a separation with poly (His) fusion proteins which are affinity chromatography tags used in His MicroSpin Purification Module, HisTrap™ Kit, HiTrap® Chelating HP and Chelating Sepharose® Fast Flow products from Cytiva.
Performing a Purification of NAD+-dependent dehydrogenases and ATP-dependent kinases with 5’ AMP Sepharose® 4B, HiTrap® Blue HP and Blue Sepharose® 6 Fast Flow
This page shows how to purify NAD+-dependent dehydrogenases and ATP-dependent kinases by affinity chromatography using 5’ AMP Sepharose® 4B, HiTrap® Blue HP and Blue Sepharose® 6 Fast Flow products from Cytiva.
Performing a Purification with Calmodulin Sepharose 4B
This page shows how to purify ATPases, adenylate cyclases, protein kinases, phosphodiesterases and neurotransmitters by affinity chromatography using Calmodulin Sepharose 4B from Cytiva.
Column Packing and Preparation for Affinity Chromatography with Specific Groups of Biomolecules
Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.
Purification using StrepTactin Sepharose® High Performance
This page shows how to purify Strep-tag II recombinant proteins using StrepTactin Sepharose® High Performance from Cytiva.
Magnetic Bead-Based Purification/Screening Using His Mag Sepharose® Ni
This page shows magnetic bead-based purification and screening using His Mag Sepharose Ni from Cytiva.
Packing Tricorn 10/100 Columns with MabSelect™ or MabSelect™ SuRe
This page shows how to pack Tricorn 10/100 columns with MabSelect or MabSelect SuRe from Cytiva.
Sample Preparation for Size Exclusion Chromatography
Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of
Purification of Histidine-Tagged Proteins using HiTrap® IMAC HP and HiTrap® IMAC FF
This page shows how to purify histidine-tagged proteins using HiTrap® IMAC HP and HiTrap® IMAC FF from Cytiva.
HIC Purification Options and Scale up with SOURCE™ Media
This page discusses how to do a purification with SOURCE™ media from Cytiva.
Gel Filtration Markers Kit for Protein Molecular Weights 12,000-200,000 Da
Gel filtration chromatography is an established method for determining the size and molecular mass of proteins.
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