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Purification using StrepTrap™ HP 1 mL and 5 mL
This page shows how to purify Strep-tag II recombinant proteins using StrepTrap HP 1 mL and 5 mL from GE Healthcare.
Performing a Separation with Sephadex®
This page shows how to perform a separation with a Sephadex column from GE Healthcare which are Size Exclusion Chromatography media consisting of an epichlorohydrin cross-linked dextran.
Purification or Removal of Albumin and IgG
This page shows how to purify or remove albumin and IgG with Albumin & IgG Depletion Sepharose High Performance from GE Healthcare.
Purification or Removal of Biotin and Biotinylated Substances
This page shows how to purify or remove biotin and biotinylated substances with Streptavidin Sepharose High Performance from GE Healthcare.
Clean-up of Antibody Labeling Reactions Using Amicon Ultra Filters
Using a standard Amicon® Ultra centrifugal filter, a few rounds of diafiltration can efficiently remove unincorporated label from an antibody labeling reaction without diluting the sample.
Rapid Antibody Concentration with Amicon® Ultra Centrifugal Filters
Purification protocols for immunoglobulins generally include affinity binding to Protein A or G chromatography media, washing unbound proteins, and eluting with a low pH buffer.
Characteristics of Glutathione Sepharose® Products
Characteristics of Glutathione Sepharose® Products
Purification Using GST HiTrap 1 mL and 5 mL Columns: GSTrap 4B, GSTrap FF, and GSTrap HP
This page describes how to purify GST-tagged proteins using GST HiTrap 1 ml and 5 ml columns: GSTrap 4B, GSTrap FF and GSTrap HP from GE Healthcare.
cOmplete™ His-Tag Purification Column Protocol & Troubleshooting
cOmplete™ His-Tag Purification Column Protocol & Troubleshooting
Column Packing and Preparation for Affinity Chromatography with Specific Groups of Biomolecules
Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.
Solubilization of Membrane Proteins
This page shows how to solubilize membrane proteins with products from GE Healthcare.
Performing a Purification of IgG Antibodies with MabSelect Sure
This page shows how to separate IgG antibodies by affinity chromatography using MabSelect Sure from GE Healthcare.
Purification of Histidine-Tagged Recombinant Proteins Using Ni Sepharose 6 Fast Flow
This page shows how to purify histidine-tagged recombinant proteins using Ni Sepharose 6 Fast Flow from GE Healthcare.
Sepharose XL – for selected proteins that require very high binding capacity to increase productivity, easy scale-up
This page covers how and when to use Sepharose XL media for purification of proteins.
Purification or Removal of Glycoproteins and Polysaccharides
This page shows how to purify or remove glycoproteins and polysaccharides with Con A Sepharose 4B, Lentil Lectin Sepharose 4B, Capto Lentil Lectin from GE Healthcare.
Purification Using GSTrap™ HP, GSTrap™ FF, and GSTrap™ 4B columns
This page shows how to purify GST-tagged proteins using GSTrap HP, GSTrap FF and GSTrap 4B columns from GE Healthcare.
Performing a Purification of IgG Antibodies with Protein G HP SpinTrap™/Ab SpinTrap™
This page shows how to separate IgG antibodies by affinity chromatography using Protein G HP SpinTrap™/Ab SpinTrap™ from GE Healthcare.
Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from GE Healthcare.
Purification using MBPTrap™ HP Columns
This page shows how to purify MBP-tagged proteins using MBPTrap HP columns from GE Healthcare.
Immunoprecipitation of FLAG Fusion Proteins Using Monoclonal Antibody Affinity Gels
Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels
Sepharose Fast Flow: Purification with Good Resolution and Easy Scale-Up
This page covers the use of Sepharose Fast Flow for purification of proteins.
Buffer Exchange and Desalting for Affinity Chromatography
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
Column Cleaning for Ion Exchange Chromatography and Chromatofocusing
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
Affinity Chromatography in a Purification Strategy (CIPP)
This page shows the purification strategy of capture, intermediate purification, and polishing (CIPP) in affinity chromatography using GE Healthcare products.
Sample Preparation for Affinity Chromatography
This page shows how to prepare samples for purification with affinity chromatography.
Sera-Mag™ SpeedBeads Magnetic Protein A/G-Particles Protocol
Sera-Mag™ SpeedBeads Magnetic Protein A/G-Particles Protocol
Purification of Histidine-Tagged Proteins using IMAC Sepharose® High Performance
Purification of Histidine-Tagged Proteins using IMAC Sepharose® High Performance
Sepharose High Performance: Purification with High Resolution
This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.
cOmplete™ His-Tag Purification Resin Protocol & Troubleshooting
cOmplete™ His-Tag Purification Resin Protocol & Troubleshooting
SOURCE™: Purification at High Throughput with High Resolution and Easy Scale-Up
This page covers using SOURCE™ medias for the purification of proteins, peptides, or oligonucleotides.