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Imprint® Chromatin Immunoprecipitation Kit (CHP1) Protocol
The Sigma Imprint Chromatin Immunoprecipitation Kit uses a plate based system to allow rapid ChIP assays in a high throughput format
Immunoprecipitation Kit (Protein A) Protocol & Troubleshooting
Immunoprecipitation Kit (Protein A) Protocol & Troubleshooting
Antibody Purification using Protein A, Protein G, or Protein L Agarose
Antibody Purification using Protein A, Protein G, or Protein L Agarose protocol is designed as a quick purification method for antibodies from mammalian sera, ascites, and cell culture supernatants. It should be noted that if the starting material is serum...
Dextran Sulfate
Dextran sulfates are supplied as the sodium salt forms, making them soluble and stable in water. Dextran sulfate contains approximately 17% sulfur which is equivalent to approximately 2.3 sulfate groups per glucosyl residue.
Purification from Unclarified Cell Lysate using HisTrap™ FF Crude
This page shows how to purify histidine-tagged recombinant proteins from unclarified cell lysate using HisTrap FF crude from GE Healthcare.
Immunoprecipitation Procedure
To determine the molecular weights of protein antigens, to study protein/protein interactions, to determine specific enzymatic activity, to monitor protein post-translational modifications and to determine the presence and quantity of proteins.
Protocols for Membrane Scaffold Proteins and Nanodisc Formation
Protocols for Membrane Scaffold Proteins and Nanodisc Formation
Performing a Purification of IgG Antibodies with MabSelect Sure
This page shows how to separate IgG antibodies by affinity chromatography using MabSelect Sure from GE Healthcare.
Performing a Purification of IgG Antibodies with Protein G HP SpinTrap™/Ab SpinTrap™
This page shows how to separate IgG antibodies by affinity chromatography using Protein G HP SpinTrap™/Ab SpinTrap™ from GE Healthcare.
Dextran
Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(1-6) linkages. The remaining a(1-3) linkages account for the branching of dextran. Conflicting data on the branch lengths implies that the average branch length is less than three...
Sera-Mag™ SpeedBeads Magnetic Protein A/G-Particles Protocol
Sera-Mag™ SpeedBeads Magnetic Protein A/G-Particles Protocol
Immunoprecipitation Kit (Protein G) Protocol & Troubleshooting
Immunoprecipitation Kit (Protein G) Protocol & Troubleshooting
Performing a Separation with GE Healthcare Products Based on GST Fusion Proteins
How to perform a separation with GST fusion proteins which are affinity chromatography tags used in GST MicroSpin Purifcation Module, GSTrap FF, GSTPrep FF 16/10, Glutathione Sepharose 4 Fast Flow and Glutathione Sepharose 4B products from GE Healthcare.