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Precipitation Procedures
This page describes protein precipitation as an optional step in sample preparation for 2-D electrophoresis with Cytiva products.
YeastBuster™ Protein Extraction Reagent for Fast, Efficient Extraction of Proteins from Yeast
YeastBuster™ Protein Extraction Reagent for Fast, Efficient Extraction of Proteins from Yeast
RNA Stabilization and Protein Stabilization in Tissue Extracts with Stabilyser™ Reagent
Stabilize complete, uniform tissue homogenates. Maintain RNA and functional proteins with Stabilyser reagent, the only reagent that protects the integrity of nucleic acid and active protein in one uniform lysate mixture.
Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens
Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens
Organelle Isolation
The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate and purify
cOmplete™ Lysis-M Protocol & Troubleshooting
cOmplete™ Lysis-M Inhibitor: Low protein concentration in lysate
Performing a Cell Lysis of Recombinant Histidine-Tagged Proteins
For optimal conditions for growth, induction, and cell lysis of recombinant histidine-tagged proteins, please refer to established procedures. The following is a general procedure for cell lysis and sample preparation from bacterial cultures. Other established procedures may also work.
Yeast Cell Lysis Reagents
Our yeast cell lysis reagents are versatile, phosphate-free, and non-denaturing. CelLytic Y Cell Lysis reagent provides an effective method for yeast cell lysis and protein solubilization. The CelLytic Y Yeast Enzymatic Lysis Kit provides a convenient method for
Roche Protein Stabilization Reagents
Get maximum protection after protein isolation by using protease and phosphatase inhibitor cocktail tablets from Roche.
Benzonase® Nuclease Q&A
This page lists nine frequently asked questions and answers about Benzonase® Nuclease.
Lysing Enzymes
Enzymes provide a non-mechanical method for cell lysis and protoplast preparation. It may seem like a simple process to throw in your enzyme, stick your tube in the water-bath and walk away, but what is actually going on in that
BugBuster® Plus Lysonase™ Kit: Cell Lysis and Nucleic Acid Removal for Gram-negative and Gram-positive Bacteria
Cell lysis and nucleic acid removal for Gram-negative and Gram-positive bacteria using the BugBuster Plus Lysonase™ Kit
Simple, efficient extraction of protein from hepatocytes with CytoBuster™ Reagent
CytoBuster Protein Extraction Reagent is a formulation of detergents prepared for the efficient extraction of soluble proteins from mammalian cells
BugBuster® and Benzonase® Reagents are the Clear Solutions to Simple, Efficient Extraction of E. coli Proteins
This article shows the use of BugBuster® and Benzonase® as protein purification tools to extract recombinant proteins from E. coli and to reduce the viscosity of the extract.
Use of BugBuster® and Lysonase™ Reagents for efficient protein extraction from Gram-positive bacteria
The combination of BugBuster® and Lysonase™ reagents greatly enhances the release of soluble proteins from Gram-positive bacteria.
Protease Inhibitor Cocktails
Protease Inhibitor Cocktails maintain and preserve cellular protein composition following cell lysis. When used with CelLytics, they also preserve target protein activity. These cocktails have been optimized for a wide variety of specific applications, ensuring excellent inhibition and ease of
Phosphatase Inhibitor Cocktail
The use of phosphatase inhibitors is critical for all types of phosphorylation studies. Compare phosphatase inhibitor cocktails to select the best one for your research.
Detergent Properties and Applications
Use detergents more effectively in cell lysis, membrane protein and lipid solubilization, protein crystallization, or Western blotting.
ReadyShield Phosphatase and Protease Inhibitor Cocktail FAQ
ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad