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Applications:Protein concentration and buffer exchange
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Buffer Reference Center
Choose a buffer based on your pH requirements as well as the pKa, a measure of acid strength that accounts for pH, concentration, and temperature.
Clean-up of Antibody Labeling Reactions Using Amicon Ultra Filters
Using a standard Amicon® Ultra centrifugal filter, a few rounds of diafiltration can efficiently remove unincorporated label from an antibody labeling reaction without diluting the sample.
Large volume concentration and diafiltration with Amicon® Stirred Cells
Demonstrating the effective use of Amicon Stirred Cell for large volume concentration through diafiltration.
Desalting Samples Using Mini Dialysis Kit
Mini Dialysis Kit from GE Healthcare Lifescience is designed for the dialysis of small sample volumes with minimal handling and sample loss and reduces the pronounced streaking on 2-D gels caused by low-molecular-weight contaminants.
Conditioning Purified Membrane Proteins
This page shows how to condition membrane proteins for further analysis with products from GE Healthcare.
Rapid Antibody Concentration with Amicon® Ultra Centrifugal Filters
Purification protocols for immunoglobulins generally include affinity binding to Protein A or G chromatography media, washing unbound proteins, and eluting with a low pH buffer.
Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from GE Healthcare.
Buffer Exchange and Desalting for Affinity Chromatography
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
Rapid, Ultrafiltration-based Method for Purification of Monoclonal Antibodies Using the Centricon® Plus-70 Device
Monoclonal antibodies (MAb) continue to gain importance as therapeutic and diagnostic agents for many cancers. The process of screening hybridoma libraries for candidate MAbs typically requires several days to complete and can be labor-intensive when evaluating multiple MAbs in parallel....
Sample Preparation for Size Exclusion Chromatography
Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of...
A Simple Strategy for Protein Enrichment Using Ultrafiltration
The human plasma proteome is a vast resource for biomarker discovery and investigation.
Detergent Removal with Amicon® Pro Centrifugal Filters
Detergent Removal with Amicon® Pro Centrifugal Filters
Simple dialysis of Small Volume Samples using V Series Membranes
This article is a protocol for Simple dialysis of small volumes using V Series membranes
Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
Extracellular Vesicle Protocol
Learn best practices for standardizing and reducing the Extracellular Vesicle Preparation workflow, and eliminating contamination of EV preparations with dead-cell-derived vesicles.