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ChIP Troubleshooting
Frequently ask questions about ChIP.
ChIC and CUT&RUN Data Analysis Tool Frequently Asked Questions (FAQs)
Chromatin Immunocleavage (ChIC) and CUT&RUN data analysis tool frequently asked questions (FAQs).
ChIC and CUT&RUN Data Analysis Tool Overview
Chromatin Immunocleavage (ChIC) and CUT&RUN data analysis tool for file management and downstream sequencing data analyses.
RNA Immunoprecipitation (RIP) FAQs
RNA Immunoprecipitation (RIP) Frequently Asked Questions
Chromatin Immunocleavage (ChIC) kits and CUT&RUN technology overview for improved chromatin isolation and downstream analyses.
PEPscreen® Peptide Library Frequently Asked Questions (FAQ)
PEPscreen peptides are synthesized on proprietary, state-of-the-art robotic platforms utilizing optimized protocols based on Fmoc-chemistry.
Duolink® PLA Flow Cytometry Kits
Traditional flow cytometry has been limited in the ability to detect protein-protein interactions and low abundant proteins events — until now. We have combined Duolink® Proximity Ligation Assay (PLA) with flow cytometry in a convenient kit, making the analysis of...
Duolink® PLA Technology for Neuroscience Applications
Duolink proximity ligation assay used to study neuron interactions furthering neuroscience research.
Sulfoxide-Containing Mass Spectrometry (MS)-Cleavable Cross-Linkers
Learn about homobifunctional sulfoxide-containing MS-cleavable cross-linkers that enables the capture of protein-protein interactions (PPIs) in native cellular environments, and the identification of cross-linked peptides permits the determination of both identity and connectivity of PPIs.
Use of PEPscreen® Peptides to Investigate Protein Farnesyltransferase Substrate Specificity
Prenylation is an essential post-translational modification for the proper localization and function of many proteins and is catalyzed by protein farnesyltransferase (FTase) and protein geranylgeranyltransferase type I (GGTase-I). To identify potential novel prenylated proteins, we used a PEPscreen peptide library...
RNA Immunoprecipitation Chip (RIP) Assay
RNA Immunoprecipitation (RIP) is an essential method for analyzing proteins that interact with and modify the function of mRNAs, small RNAs, viral RNAs, or lncRNAs.
ChIP: Immunoprecipitation, washing and elution
Chromatin Immunoprecipitation (ChIP), washing and elution
Duolink® References
Find Duolink references based on the type of method used, post translational modification detected, and research focus.
How Proximity Ligation Assays (PLA) Work
Learn how Proximity Ligation Assay technology works and how the protein-protein interaction control kit can confirm in situ detection of EGF-induced EGFR-HER2 dimerization.
Troubleshooting Guide for Duolink® PLA for Flow Cytometry Detection
General tips and tricks for proper experiment execution, aid in identifying potential problems, and provide solutions to ensure a successful Duolink® PLA experiment for flow cytometry.
Use of PEPscreen® Libraries to Identify CD8 Epitopes Presented During Malaria Infection
Malaria caused by Plasmodium species kills more than one million people per year, 90% of them in sub-Saharan Africa. Our previous work had shown that CD8 T cells are a major component of immunity to the first stage of infection...
Duolink® PLA – Protein Detection Technology
Duolink based on Proximity Ligation Assay (in situ PLA) technology when combined with qualified antibodies outperforms traditional immunoassays, such as co-IP, through a simple and sensitive in situ detection of endogenous proteins. This technology enables you to visualize protein interactions...
Detection of protein-protein interactions by in situ PLA
Detect protein-protein interactions with Duolink® in situ PLA. Highly characterized antibodies are crucial for achieving a successful PLA reaction.
Duolink® Proximity Ligation Assay Instructional Video
This video will show you step-by-step how to perform a Duolink® PLA experiment with cells on a slide that have already been fixed and permeabilized by the user.
ChIP Troubleshooting Tips
Chromatin Immunoprecipitation (ChIP) Troubleshooting Tips
FLAG® HA System
The FLAG® HA tandem epitope system consists of small epitopes tags that are not eukaryotic derived. These features minimize interference with protein functions and provide superior specificity.
How to Optimize the Duolink® Proximity Ligation Assay
Things to consider for preparation, setup and execution of the Duolink® assay protocol
ChIP-X: Cross-linking proteins to DNA and cell lysis
X-ChIP: Cross-linking proteins to DNA and cell lysis
Use of PEPscreen® Peptides to Investigate CTL Class I MHC and Th Class II Responses to AAV-hF.IX Gene Therapy
Recombinant adeno-associated virus (AAV)-mediated gene therapy expressing human coagulant factor IX (hF.IX) is a promising gene transfer strategy due to the long-term expression of hF.IX in the liver and weak immune response using a liver-specific promoter. However, in humans and...
Primary Antibodies for use with Duolink® PLA
Search for PLA validated primary antibodies and use with Duolink® PLA to amplify the signal 1000x. You can visually detect endogenous proteins in native cells.
Guides to cell number for ChIP and endpoint analysis
Guides to cell number for Chromatin Immunoprecipitation (ChIP) and endpoint analysis
How to Optimize Duolink® PLA for Flow Cytometry Detection
Considerations for proper experimental design, preparation and execution of the Duolink® PLA for flow cytometry protocol.
Duolink® Proximity Ligation Assay (PLA) Multicolor Kits for Sensitive Multiplex Detection
Duolink® PLA Multicolor technology and the quantification of protein-protein interactions in fixed tissues and cells.
Studies of Dimerization and Activation of Receptors in the EGFR-Family Using Duolink® Assays
Our aim was to investigate the dimerization and activation pattern of two receptors, EGFR (ErbB1) and HER2 (ErbB2), in the EGFR-family using breast cancer cell lines and selected colon tissues as model systems.
Fragmentation of chromatin in ChIP
Fragmentation of chromatin in Chromatin Immunoprecipitation (ChIP)
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