Applications
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Oligonucleotide Quantification
DNA absorbs ultraviolet light due to its highly conjµgated nature. DNA may thus be easily quantitated in a UV spectrometer.
DNA Oligonucleotide Synthesis
Learn about the steps in phosphoramidite solid-phase method of Oligonucleotide Synthesis method that we use for DNA oligonucleotide manufacturing.
Restorase® DNA Polymerase
Restorase® was developed for researchers unable to achieve amplification of damaged DNA templates when using other commercially available DNA polymerases.
Whole Transcriptome Amplification of RNA from Low Cell-Number Samples
The efficacy of amplification of small quantities of total RNA with the Complete Whole Transcriptome Amplification Kit (WTA2) was examined in this study.
KAPA2G Fast PCR Kits FAQ
Frequently asked questions for KAPA2G Fast PCR Kits.
Polymerase Chain Reaction
The polymerase chain reaction is one of the most widely used techniques in molecular biology. The PCR process consists of three main steps, Denaturation, Annealing & Extension
Oligonucleotide Array CGH Analysis of a Robust Whole Genome Amplification Method
In recent years, array-based Comparative Genomic Hybridization (aCGH) has been refined to determine chromosomal changes at progressively higher resolutions. This evolving technology is, however, somewhat hampered by the large DNA input requirement—a minimum of 150,000 copies of a human genome...
Qualitative multiplex PCR assay for assessing DNA quality from FFPE tissues and other sources of damaged DNA
The assessment of DNA quality is a crucial first step in acquiring meaningful data from formalin-fixed paraffin-embedded (FFPE) tissues, and other sources of damaged DNA. Using intact genomic DNA is key for successful analysis of chromosomal aberrations (e.g. SNP analysis...
Extract-N-Amp™ Tissue Feature Article
The Extract-N-Amp™ Tissue PCR Kit has been created to release PCR-ready DNA from mouse tails in a 15 minute single tube procedure. The included 2x PCR mix is optimized to work with the crude extracts and the solutions in the...
Troubleshooting PCR and RT-PCR Amplification
This page shows PCR and RT-PCR amplification troubleshooting.
What is Hot Start PCR?
Learn about the benefits of using Hot Start PCR reagents to suppress enzymatic activity before PCR reaction tubes enter the thermal cycler.
Polymerase Chain Reaction (PCR) Basics
Polymerase chain reaction (PCR) is a technique that results in exponential amplification of a target DNA sequence.
PCR-based Assay Regulations and Validation
While many PCR assays are developed for research applications there are further considerations for those that are being developed to become diagnostic assays or to be performed in support of: Biologics License Application (BLA), New Drug Application (NDA), Premarket Approval...
PCR/qPCR/dPCR Assay Design
The entire PCR workflow is vulnerable to factors which introduce variability. Many of the variable components are unavoidable, such as the source of the sample or the requirement for a reverse transcription step. Assay design is also highly variable and...
Retinoic Acid and Gene Expression
All-trans retinoic acid (RA, ATRA) is a pleiotropic activation factor that regulates genes associated with normal vertebrate cellular processes such as cell differentiation, cell proliferation, apoptosis, and embryonic development.
Extract-N-Amp™ Tissue Feature Article
Standard methods for extracting DNA from tissues can be extremely laborious and time consuming. Certain applications, such as genotyping of transgenic mice using a section of tail, employ a lengthy DNA extraction process.
KAPA Long Range PCR Kits FAQs
Frequently asked questions (FAQs) for KAPA Long Range PCR Kits.
Low Contaminant Amplification of DNA Using MTP™ Taq DNA Polymerase (D7442)
Method for bacterial genome analysis and detection of pathogens. Minimize false positive PCRs through lab design and reagents tested for use in bacterial PCR applications.
Extract-N-Amp Reagent Provides a Highly Effective Way of Generating PCR Products from FTA® Blood Cards
Extract-N-Amp is a versatile, combined DNA extraction and amplification kit intended to simplify the generation of PCR products from a range of sample types. This methodology can be used without modification to perform PCR on blood samples stored on FTA...
5'/3' RACE Kit, 2nd Generation Troubleshooting
5'/3' RACE Kit, 2nd Generation Troubleshooting
Roche PCR Reagents and PCR Protocols
Roche polymerase chain reaction (PCR) reagents for reducing the error rate of Taq DNA polymerase and PCR selection guide.
ThermaStop-RT PCR Additive
PCR additive interacts with reverse transcriptase at low temperatures to reduce priming problems that lead to nonspecific bands.
Dual-Labeled Probes in Digital PCR
Dual-Labeled Probes in Digital PCR
OligoArchitect™ Assay Design
Efficient qPCR relies on good assay design. Since the invention of PCR, many parameters have been identified as important for assay quality, such as estimates of oligo temperature characteristics, GC content and folding properties.
PCR/qPCR Data Analysis
After a traditional PCR has been completed, the PCR/qPCR data analysis is conducted by resolution through an agarose gel or, more recently, through a capillary.
Oligonucleotide Quality Control by Mass Spectrometry
Mass Spectrometry is the technology of choice for analyzing oligonucleotide synthesis. It enables the most sensitive detection of low levels of by-products, which can affect performance.
KAPA3G Plant PCR Kits FAQs
Frequently asked questions (FAQs) for KAPA3G Plant PCR Kits.
Extract-N-Amp™ Plant Tissue PCR Kits
The Extract-N-Amp™ kits are designed to rapidly extract and amplify genomic DNA. The plant tissue version of these kits has been optimized to amplify without concern over plant inhibitors. This technical document will discuss the versions of this kit that...
Long and Accurate PCR
Long and accurate PCR applications address the needs for longer read lengths, greater fidelity and higher yields than that which can be achieved with Taq DNA polymerase.
Quantitative PCR and Digital PCR Detection Methods
Fluorescent dyes or probes are included in PCR mixes to monitor the change in NA amplicon concentration as the reaction proceeds.