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Selecting Correctly Expressing Recombinants
Blue White Screening; DNA Minipreps; Screening by Restriction Digestion; Screening by PCR; Confirm cut plasmid sizes by agarose gel electrophoresis; DNA Maxipreps; DNA Precipitation; RNase Treatment; Clean-up of DNA
Buccal DNA Extraction & WGA Amplification Protocol
This protocol provides a simple and convenient method to isolate, amplify, and purify genomic DNA from buccal swabs. Buccal swabs are a convenient method of acquiring a DNA sample.
Protocol for Conjugating NHS-Ester Modifications to Amino-Labeled Oligonucleotides
This protocol is for conjugating NHS-ester modifications to an oligonucleotide with an amino label, such as 5'-Amino-Modifier C6.
Plant DNA Extraction & WGA Amplification Protocol
GenomePlex® Whole Genome Amplification has been used to amplify genomic DNA from soybean, corn, tomato, purple coneflower, and ginseng.
Whole Genome Amplification: Extraction Protocol - Blood Card
Whole Genome Amplification: Extraction Protocol - Blood cards provide the convenience of archiving small volumes of blood. However, many times genomic DNA from these samples is limited, which may hinder the researcher’s ability to perform downstream analysis. This protocol provides...
Deoxyribonuclease I
Deoxyribonuclease I is isolated from bovine pancreas and is processed to reduce RNase activity to below detectable levels.
REDExtract-N-Amp™ Tissue PCR Kit Protocol
The exrract-N-Amp kit protocol provides a rapid DNA extraction method that leads to a PCR-ready sample in 15 minutes. Optimized PCR ReadyMix yields successful and clean amplification. RED loading dye allows for sample tracking.
End-Point PCR: Antibody-Mediated Hot Start PCR Protocol with Enhanced Specificity and Yield
Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
Standard PCR Protocol
Learn standard PCR protocol steps and review reagent lists or cycling parameters. This method for routine PCR amplification of DNA uses standard Taq DNA polymerase.
Extract-N-Amp™ Plant PCR Kits Protocol
The Extract-N-Amp™ Plant PCR Kits contain all of the reagents required to rapidly extract and amplify genomic DNA from plant leaves.
Applications with REDTaq™
Reviews the applications and benefits for RedTaq, including standard RedTaq, Hot Start RedTaq and RedTaq for genomic DNA PCR.
Webinar: High-precision PCR using ThermaGenix Reagents
Webinar: High-precision PCR using ThermaGenix Reagents
Amplification of Genomic DNA using REDTaq DNA Polymerase
Method for PCR using genomic DNA or other complex templates
Whole Blood
Whole blood is a common source of material used to perform genetic analysis. Many times genomic DNA isolated from whole blood samples is of low yield.
End Point PCR Protocol for Long and Accurate DNA Amplification
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.
Long and Accurate PCR Amplification of DNA (D8045)
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. AccuTaq LA.
Hot Start dNTP protocol to reduce non-specific amplification
Protocol using hot start dNTPs. Method includes modified nucleoside triphosphates that block DNA polymerase nucleotide incorporation during hot start PCR to increase specificity. Compatible with a variety of PCR reagents.
dNTP Mediated Hot Start PCR Protocol
Hot Start dNTPs are modified with a thermolabile protecting group at the 3’ terminus. The presence of this modification blocks nucleotide incorporation by DNA polymerase until the nucleotide protecting group is removed during a heat activation step.
Multiplex Real-Time PCR
Multiplex qPCR employing probe-based chemistries is a demanding application that often requires extensive optimization and validation.
Genomic DNA Extraction and Amplification Procedure using REDExtract-N-Amp™ Tissue PCR Kit
he REDExtract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
Protocol for DNA extraction & WGA Amplification from Soil Samples
Genomic DNA from soil samples can be easily damaged by nucleases and contaminating debris resulting in low DNA yields. As a result, the researcher’s ability to perform downstream analysis may be compromised. After isolating DNA from the soil sample, the...
Extract-N-Amp™ Tissue PCR Kit Protocol
The Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
FFPE Tissue
Archived Formalin-fixed, Paraffin-embedded (FFPE) tissue samples are invaluable resources for profiling gene expression and studying a variety of diseases.
Extract-N-Amp™ FFPE Tissue PCR Protocol
Extract-N-Amp™ FFPE Tissue PCR Protocol
Expand™ Long Template PCR System Protocol & Troubleshooting
Expand™ Long Template PCR System Protocol & Troubleshooting
Kapa Biosystems Taq DNA Polymerase and PCR Kits
Robust Taq DNA polymerase reagents and PCR kits for efficient extraction, purification, and amplification of routine and challenging DNA template sequences.
Whole Gene Amplification Formalinfixed Paraffin embedded Tissues
Archived formalin-fixed and paraffin-embedded (FFPE) tissue collections represent invaluable resources for studying pathogenesis of cancer and a variety of other diseases.
Annealing Oligonucleotides Protocol
Annealing is the process of heating and cooling two single-stranded oligonucleotides with complementary sequences. Heat breaks all hydrogen bonds, and cooling allows new bonds to form between the sequences.
Titan™ One Tube RT-PCR System Protocol & Troubleshooting
Titan One Tube RT-PCR System Protocol & Troubleshooting
SEQR - SeqPlex RNA Amplification Kit Protocol
The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms.