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Autophagy Mitophagy in Tumor Cell Survival Death
Autophagy is a highly regulated process that is involved in cell growth, development, and death. In autophagy cells destroy their own cytoplasmic components in a very systematic manner and recycle them.
Autophagy in Cancer Promotes Therapeutic Resistance
We presents an article on Autophagy in Cancer Promotes Therapeutic Resistance
Insulin-like Growth Factors (IGF)
Insulin-like Growth Factors (IGF)
Folic Acid Metabolism in Cancer
This issue of Biofiles reviews some of our newest and most innovative technologies and their specific applications toward cancer research. In preparing this issue of Biofiles, one is reminded how complex the disease of cancer is, and how difficult it...
Insulin Signaling and Energy Homeostasis
Glucose metabolism is regulated by the opposing actions of insulin and glucagon. Insulin is released from pancreatic ß cells in response to high blood glucose levels and regulates glucose metabolism through its actions on muscle, liver, and adipose tissue.
Inhibition of Cell Wall Biosynthesis by Antibiotics
Inhibition of Cell Wall Biosynthesis by Antibiotics
Using Immortalized 16HBE14o- Human Bronchial Epithelial Cell Lines to Model Respiratory Lung Diseases
16HBE14o- human bronchial epithelial cells used to model respiratory epithelium for the research of cystic fibrosis, viral pulmonary pathology (SARS-CoV), asthma, COPD, effects of smoking and air pollution. See over 5k publications.
Cell Analysis Best Practices
How do you assess the state of your cell culture? We’ve got some best practices to share.
NF-κB and Inflammation
NF-κB and Inflammation
Epidermal growth factor receptor (EGFR) signaling
Read review on EGF/EGFR signaling (epidermal growth factor receptor) and find links to related products at sigmaaldrich.com
Lipid Induced Insulin Resistance
Lipid Induced Insulin Resistance
Cell Culture FAQs: Bacterial Endotoxin Contamination
What is endotoxin? Frequently asked questions about bacterial endotoxin contamination of in vitro cell cultures. Details about how to endotoxin test using the LAL assay, common sources of laboratory endotoxin contamination and tips on how to avoid endotoxin contamination when...
Canonical Wnt / β-Catenin Signaling
We review the Wnt signaling pathway (also, canonical Wnt or beta (β)-catenin pathway) and provide links to related products at sigmaaldrich.com.
Corning® High Content Screening Microplates – Frequently Asked Questions
A frequently asked questions guide for users of the Corning® high content screening microplates.
General Adherent Cells
Protocol for creating create stable cell lines from both dividing and non-dividing cells for use with a lentiviral delivery system.
Transferrin in Cell Culture
How transferrin and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page introduces the in vitro chemistry and biochemistry of transferrin.
Cell Culture Contamination Troubleshooting
Common Cell Culture Problems: Contamination is easily the most common problem encountered in cell culture laboratories, sometimes with very serious consequence.
Poor Adherent Cell Attachment Troubleshooting
Cells that are cultured under suboptimal growth conditions attach poorly. When the culture surface provided is appropriate, the most common cause of failure of cells to attach to a substrate is environmental stress placed on the cells.
Serum for Cell and Tissue Culture FAQs
Find answers to frequently asked questions (FAQs) about FBS and other serum products, and links to related products.
Cell Culture Protocol 5: Subculture of Suspension Cell Lines
Cell culture protocol for passaging and splitting suspension cell lines
Identification of Cell Culture Media with Raman Spectroscopy
Identification of cell culture media samples by traditional LC methods, such as amino acid or vitamin analysis, has high costs, and requires significant analytical expertise and laboratory space.
Vitamin B12, Cobalamin, in Cell Culture
Importance and uses of Vitamin B12 in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell cultures
Uric Acid in Cell Culture
Uric acid is not a direct additive to any of the classical cell culture media. However, it is present in animal sera. Consequently, uric acid is typically present in complete serum-supplemented classical cell culture systems.
Cell Counting Using a Hemocytometer
Cell counting protocol using a hemocytometer and trypan blue for measuring cell viability.
Lipoic (Thioctic) Acid, in Cell Culture
How lipoic acid (thioctic acid) and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies.
Stearic Acid in Cell Culture
Fatty acids of the n-3, n-6 and n-9 families are important supplements for cell culture systems. They are important in cell culture systems used to biomanufacture heterologous proteins, such as monoclonal antibodies.
Common Cell Culture Problems: Precipitates
Common Cell Culture Problems: Precipitates -- When contamination is ruled out, turbidity in cell culture media is often explained by the precipitation of metals, proteins, and other media components. Precipitates can be harmful to cell health, as they may alter...
Cell Culture Fundamentals: Cryopreservation and Storage of Cell Lines
Overview on liquid nitrogen storage and freezing of cell stocks for cell line cryopreservation purposes. Free ECACC handbook download.
Alternative High-Yield Cell Culture Systems
Solutions for high-yield cell culture scale-up applications including multi-layered flasks (HyperFlasks/CellStacks), roller bottles, spinner and shaker flasks. Free ECACC handbook download.
How to Fractionate and Analyze Gradients of Percoll
Density Marker Beads are dyed derivatives of Sephadex™. There are ten color-coded bead types, each with a specific density. They have been specifically formulated for use in Percoll gradients and will not work with other media. Using Density Marker Beads...