Applications
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Showing 1-28 of 28 results
Fibronectin Coating Protocol
Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs at sigmaaldrich.com
Thawing of Frozen Cell Lines
Cell culture protocol for proper thawing of cryopreserved cell lines. Free ECACC handbook download.
Gelatin Coating Protocol
Gelatin coating protocol, products, and FAQs.
Enzymatic Method for Determining Glucose and Sucrose (Glucose and Sucrose Assay)
Assay protocol for the colorimetric/fluorometric detection of glucose and sucrose in biological samples using the glucose and sucrose assay kit.
HL-1 Cells
We’ve got everything else you’ll need to get started in your research of your HL-1 cells. From culture plates and cryovials to the proprietary Claycomb Media, look no further for a complete offering of products and services to support your...
Coating Tissue Culture Plates with Vitronectin
Vitronectin is used to coat culture vessels for cells attachment.
Trypsin Inhibitors
Natural trypsin Inhibitors also known as serine protease inhibitors (serpins) are the largest and most diverse family of protease inhibitors. Serpins control the activation and catabolism of proteins by the inhibition of serine proteases in vivo.
Powdered Media Preparation Instructions
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
A Guide to Successful Cell Culture
Guidelines and best practices, brought to you by ECACC and us.
Cultrex® Basement Membrane Extract, Type 2, PathClear® Protocol
Cultrex® Basement Membrane Extract (BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor.
Subculture of Adherent Cell Lines
Cell culture protocol for passaging and splitting adherent cell lines using trypsin EDTA. Free ECACC handbook download.
Testing Cells for Bacterial and Fungal Contamination
Cell culture protocol for testing cell lines for bacterial and fungal contamination. Free ECACC handbook download.
Fibronectin Coating Protocol
Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.
Fibronectin Coating Protocol
Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs
Poly-L-Lysine Cell Attachment Protocol
Poly-L-Lysine Cell Attachment Protocol
StableCell™ Trypsin Solutions
StableCell™ Trypsin solutions are designed to perform cell detachment as standard trypsin solutions do, without the need to aliquot, freeze, and thaw. This saves significant time before passaging.
Basic Indirect Co-culture on Both Sides of Millicell® Cell Culture Insert Membranes
This page covers the basic indirect co-culture procedure on both sides of Millicell cell culture insert membranes.
Laminin Coating Protocol
Laminin coating protocol, products and FAQs at sigmaaldrich.com
Enzymatic Glutamate Assay
Assay protocol for the colorimetric detection of Glutamate in biological samples using the Glutamate assay kit.
Indirect Co-culture of Embryonic Stem Cells with Embryonic Fibroblasts
This page covers the indirect co-culture of embryonic stem cells with embryonic fibroblasts.
How to make and use gradients of Percoll
How to use percoll to prepare a gradient
Cell Dissociation Protocol with Trypsin
Trypsin is frequently used in cell dissociation from adherent surfaces. We offer a wide variety of trypsin solutions to meet your specific cell line requirements, as well as protocols, troubleshooting ideas, and more.
Canine Osteoblasts (CnOb) Culture Protocol
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
Cell Culture Protocol 4: Subculture of Semi-Adherent Cell Lines
Cell culture protocol for passaging and splitting semi-adherent cell lines using trypsin EDTA. Free ECACC handbook download.
Cryopreservation of Cell Lines
Cell culture protocol for freezing cell lines at high cell viabilities using cryopreservation reagents such as Sigma DMSO. Cryopreservation is a method whereby cells are frozen, maintaining their viability, until they are defrosted months or years later. Free ECACC handbook...
Removal of Adherent Cells from a Culture Surface Using Trypsin
Trypsin may be used to remove adherent cells from a culture surface. Cells are most commonly removed from the culture substrate by treatment with trypsin or trypsin/EDTA solutions.
Cell Culture Protocol 1: Proper Aseptic Technique and Sterile Handling of Cells
Cell culture protocol for proper aseptic technique to maintain a sterile environment and to prevent cell culture contamination. Free ECACC handbook download.
Dextran
Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(1-6) linkages. The remaining a(1-3) linkages account for the branching of dextran. Conflicting data on the branch lengths implies that the average branch length is less than three...