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Maintenance of Multimodal Chromatography Media and Storage Conditions
This page describes the maintenance of media and storage conditions for multimodal chromatography using Cytiva products.
Troubleshooting Guide for Affinity Chromatography of Tagged Proteins
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.
Characteristics of Dextrin Sepharose® High Performance Products
This page shows the characteristics of Dextrin Sepharose High Performance products from Cytiva.
Capto Adhere Anion Exchanger for MAbs & Bind/Elute
Capto adhere from Cytiva is a multimodal strong anion exchanger for BioProcess applications.
Performing a Purity and Homogeneity Check
This page shows how to perform a purification and homogeneity check of membrane proteins with products from Cytiva.
Capto MMC
Capto MMC is a multimodal cation exchanger with the properties of a weak cation exchanger. In addition to electrostatic interactions, the ligand structure provides for additional interaction modes such as hydrophobic interaction, hydrogen bonding, and thiophilic interaction.
Desalting and Buffer Exchange for Affinity Chromatography of Antibodies
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
Non-volatile and Volatile Buffer Systems
This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.
Immobilized Metal Chelate Affinity Chromatography (IMAC)
This page shows how to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography using HiTrap Chelating HP, Chelating Sepharose Fast Flow,His MicroSpin Purification Module or HisTrap Kit from Cytiva.
Lewatit® Ion Exchange Resins – All-around Solutions for Chemical Processes
Chemical processes comprise the steps of processing chemical starting materials, synthesizing the products, isolating the generated materials from the reaction mixture and subsequent purification.
QuEChERS Cleanup and Analysis of Veterinary Drugs in Milk by LC/MS/MS
QuEChERS Cleanup and Analysis of Veterinary Drugs in Milk by LC/MS/MS
HPLC analysis of Letrozole and its impurities by following the USP Guidelines Using a Purospher® STAR RP18e Column and UV Detection
An application note concerning the HPLC analysis of letrozole and its impurities by following the USP43-NF38 Guidelines using a Purospher® STAR RP18e Column.
Determination of Chlorogenic acid and Baicalin in compounded Lonucerae Japonicae Flos with Purospher® STAR RP-18e
Extraction and analysis of chlorogenic acid and baicalin active ingredients in traditional Chinese medicine (TCM) according to Chinese pharmacopeia monograph by HPLC with Purospher® RP-18e column
UHPLC Analysis of Vitamin D2 & D3 Metabolites and Epimers on Supel™ Carbon LC Columns
Ultra high-performance liquid chromatographic (UHPLC) separation of Vitamin D Metabolites― 3-epi-25-hydroxyvitamin D2 and 25-hydroxyvitamin D2, using Supel™ Carbon LC column with baseline separation, excellent peak shape, and sensitivity.
UHPLC Analysis of Ephedrine and Related Compounds on Supel™ Carbon LC Column
Ultra-high performance liquid chromatographic (UHPLC) separation of ephedrine and its three structural analogs based on polar retention effect on graphite (PREG) mechanism using Supel™ Carbon LC column.
Determination of Ephedrine HCl and Pseudoephedrine HCl in Xiao’er Kechuanling Oral Solution using a Discovery® HS C18 Column
Developing an HPLC method for quantifying ephedrine HCl and pseudoephedrine HCl in traditional Chinese medicine (TCM) with a Discovery® C18 column to report: chromatographic data, linearity, specificity and repeatability, LOD and LOQ.
LC-MS/MS Analysis of Bile Acids using Ascentis® Express C18
Isobaric separation of bile acids and conjugates by LC-MS/MS on Ascentis® Express C18 column with excellent resolution and linearity.
UHPLC Analysis of Paraquat and Diquat on Supel™ Carbon LC
Ultra-high performance liquid chromatographic (UHPLC) separation of polar herbicides, paraquat and diquat using SupelTM Carbon LC column with baseline resolution, excellent peak shape, and good lot-to-lot reproducibility.
Ion Exchange Chromatography
Ion exchange chromatography is employed by the Genopure Plasmid Midi Kit and the Genopure Plasmid Maxi Kit.
Comparison of Spherical and Irregular Silicas in Flash Chromatography
Comparison of Spherical and Irregular Silicas in Flash Chromatography
Purification of Thiol-Containing Substances By Covalent Chromatography
Thiol-containing substances can be isolated selectively by covalent binding to an activated thiolated matrix via thiol-disulphide exchange to form a mixed disulphide bond. After washing away unbound material, the thiol-containing substance is eluted by reducing the disulphide bond. This technique
Viral Clearance Using Capto™ Adhere
Description of viral clearance using Capto adhere from Cytiva.
LRA (Lipid Removal Agent) for Biopharmaceutical Production
Lipids are naturally-occurring hydrocarbons that are poorly soluble in water and soluble in non-polar solvents such as ether and chloroform.
Bind/Elute vs Flowthrough Mode in Multimodal Chromatography
This page describes the difference between bind/elute and flowthrough modes in multimodal chromatography using Cytiva products.
Troubleshooting Reversed Phase Chromatography (RPC)
Poor-quality eluent components can cause a phenomenon referred to as “ghosting”. Trace levels of organic impurities bind to the medium, concentrating during equilibration and sample application. When elution begins, these contaminants appear in the chromatogram as unknown, or “ghost” peaks.
Setting Column Pressure Limits for Size Exclusion Chromatography
Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.
Optimization of Loading Conditions on Capto Adhere Using DoE
This page describes the optimization of loading conditions on Capto adhere from using full factorial design of experiments (DoE).
Selection of Purification Equipment
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
Protein G and Protein A Bind to Different IgG
This page shows a comparison of the relative binding strengths of protein G and protein A to different immunoglobulins.
Determination of Ephedrine HCl and Pseudoephedrine HCl in Xiao’er Kechuanling Oral Solution with Purospher® STAR RP-18e colμmn
HPLC method to analyze ephedrine HCl and pseudoephedrine HCl in traditional Chinese medicine (TCM) with a Purospher® RP-18e colμmn to report chromatographic data, linearity, specificity and repeatability and LOD and LOQ.
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