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Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose® Products
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.
Absolute Quantification of Serum Proteome
Plasma proteomics holds great promise for the future of biomarker discovery, as well as in vitro diagnostics. Although plasma is readily accessible for analysis, the study of the plasma proteome is fundamentally limited by its vast dynamic range (10
Quantification of Infliximab in Human Serum by LC-MS/MS Using A Full-Length Stable Isotope Labeled Internal Standard
Infliximab, a chimeric monoclonal antibody, is used to treat rheumatoid arthritis, psoriatic arthritis, Crohn’s disease, and other autoimmune diseases.
Methods and Matrices for Mass Spectrometry of Glycans
Glycosylation is known to have profound influence on various physiochemical, cellular and biological functions of proteins. Alterations in this modification are known to affect the immune system and have been associated with various pathological states such as cancer, rheumatoid arthritis
SupraSolv® GC Solvent Specifications
Our GC solvents have been specially developed and tested for ECD, FID and MS detection or for headspace gas chromatography to ensure accurate, reliable and reproducible analytical results.
Large Molecule BioAnalysis
Bioanalysis for large molecules, like Proteins or specific Biotherapeutics, refer to a set of methods, assays, and procedures that enable scientists to analyze specific proteins found in living organisms and the biochemical reactions underlying life processes.
New Certified Reference Materials for α‑Lactalbumin, β‑Lactoglobulin A & B, and Lactoferrin
We have developed Certified Reference Materials (CRMs) of fully intact milk proteins that allow accurate testing of the most critical proteins in infant formula; help labs to develop and test infant formula products with high accuracy, while ensuring legal and
Development of Simple and Rapid Workflows for Quantitation of Infliximab and Adalimumab in Human Serum by LC-MS/MS
Development of Simple and Rapid Workflows for Quantitation of Infliximab and Adalimumab in Human Serum by LC-MS/MS
HPLC Troubleshooting Guide
Identify and correct common HPLC problems to restore column performance. Get help with peak problems (peak splitting, peak tailing, peak fronting, etc.), retention time, purge noise, and loss of resolution with example chromatograms.
HPLC Analysis of Proteins by Cation Exchange on Proteomix® WCX: Affect of Particle Size
Separation of Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein; α-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder; Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis;
BIOshell™ A400 Protein C4 U/HPLC Columns
BIOshell™ A400 Protein C4 columns contain 3.4 μm particles with 400 Å pores, which are derivatized with dimethylbutyl silane and exhaustively endcapped for optimum protein recovery.
An LC/MS Peptide Standard for Rapid System Suitability Assessment and Retention Time Translation Across LC/MS Platforms
Standards are critical in liquid chromatography/mass spectrometry (LC/MS) based proteomics to ensure optimal and consistent system performance before, during, and after sample analysis.
Application Note – Peroxidase in Vegetables
Application Note: Peroxidase in vegetables – Visual determination after conversion of a specific substrate.
Fast Analysis of Monoclonal Antibodies Using Silica Monoliths Designed for Bioanalysis
Silica monoliths with larger mesopores are a powerful tool for the fast separation of biomolecules including mAbs. The immobilization of protein A on epoxy-modified widepore (WP) silica rods enabled highly reproducible and fast, one minute, separations of mAbs. The Chromolith®
LC/MS Analysis of Illicit Bath Salts on Ascentis® Express HILIC, Fast Analysis
Separation of Butylone hydrochloride solution, 1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material; Ethylone hydrochloride, 1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material; Mephedrone hydrochloride solution, 1.0 mg/mL
A Quantitative Mass Spectrometry-based Approach for Identifying Protein Kinase Clients and Quantifying Kinase Activity
A quantitative mass spectrometry-based approach for identifying protein kinase clients and quantifying kinase activity
High Resolution and High Efficiency Separations of mAbs and ADCs Using Proteomix HIC Columns
Hydrophobic interaction chromatography, a protein separation technique, has been commonly used as an orthogonal method to SEC and ion exchange for the characterization of mAbs.
Physical Stability of a Gel Filtration Column for Antibody Analysis
The results of this column stability study show that TSKgel G3000SWXL are highly stable; the efficiency and peak symmetry values show minimal variation from the first to the 1,000th injection.
HPLC Analysis of Sugars by HILIC Chromatography using Ascentis® Express OH-5 Column with ELSD Detection
Separation of Dextrose, Pharmaceutical Secondary Standard; Certified Reference Material; Sucrose; Maltitol, Pharmaceutical Secondary Standard; Certified Reference Material
Optimising the LC-MS Analysis of Biomolecules
Sigma-Aldrich.com presents an article concerning optimising the LC-MS analysis of biomolecules.
Ascentis® Express ES-Cyano Reversed Phase U/HPLC Columns
Ascentis® Express ES-Cyano HPLC column is a high-speed, high-performance liquid chromatography column based on 90 Å Fused-Core® particles.
Monolithic Silica with Bimodal Pore Structure
Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.
Developing HPLC Methods
We recommend every HPLC method developer have these four columns in their arsenal; Ascentis C18, Ascentis RP-Amide, Ascentis Phenyl and Discovery HS F5.
UHPLC/MS Analysis of Diazepam and Metabolites on Titan™ C18, Comparison of Flow Rate and Column Length
Separation of Oxazepam; Desmethyldiazepam, analytical standard; Temazepam, analytical standard; Diazepam
Ascentis® Express Biphenyl UHPLC and HPLC Columns
The bonded, densely endcapped, dimethyl-biphenyl stationary phase of Ascentis® Express 90 Å Biphenyl, based on fused-core particles, provides a stable, reversed phase packing with enhanced π-π and mild steric interactions due to the two sequential phenyl groups bonded to the
UHPLC/MS Analysis of Spice Cannabinoids on Titan™ C18
Synthetic cannabinoids (e.g. "Spice") are a type of designer drug that provides a cannabis-type high.
Determination of Sucralose in Soy Sauce using HPLC-ELSD following the Chinese National Standard (GB) Method
The most frequently used synthetic sweeteners are: saccharin, cyclamate, aspartame, and sucralose (E955), and this application illustrate the analysis of sucralose from soy sauce following the current Chinese national standard method.
HPLC Analysis of Xanthines on Purospher® STAR RP-18 endcapped
Xanthine is a purine base found in most human body tissues and fluids as well as in other organisms. Methylated xanthines (methylxanthines), which include caffeine, paraxanthine, theobromine, and theophylline, commonly used for their effects as mild stiµlants and as bronchodilators
Ascentis® Express C8 U/HPLC Columns
When a C18 doesn′t give the desired separation, or your sample contains compounds that are known to be difficult to retain or resolve on a C18, consider changing to an Ascentis® Express C8 column.
LC/MS Analysis of Nicotine and Metabolites on Kromasil® Eternity™ C18 (Analyte Set 1)
Separation of (+)-Anabasine hydrochloride solution, 1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material; S(−)-Nicotine solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material; (S)-N-Nitrosoanabasine (NAB) solution, 1.0 mg/mL in methanol, ampule
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