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Molecular Beacons
Molecular Beacons are structured probes that are highly sensitive, sequence specific, and are used for sequence detection in qPCR and in vitro studies.
Dual-Labeled Probes
Dual-Labeled Probes are the most common probe type for qPCR and are often referred to as hydrolysis probes.
Safe Lentivirus Handling
Our lentiviral vector systems are developed with enhanced safety features. Numerous precautions are in place in the design of our lentiviruses to prevent replication. Good handling practices are a must.
What Is ZFN Technology?
Zinc finger nucleases (ZFNs) are a class of engineered DNA-binding proteins that facilitate targeted editing of the genome by creating double-strand breaks in DNA at user-specified locations.
Genome-wide Collection of Lenti 3’UTR Reporters for Functional Validation of microRNA Targets
Sigma-Aldrich presents an article about microRNAs (miRNAs), which are a class of naturally occurring small non-coding RNA molecules that regulate a variety of developmental and physiological processes.
MISSION® shRNA Lentiviral Vectors Summary
RNA interference technology has been one of the key biological breakthroughs of the last decade and has revolutionized basic biology and gene function studies, but it also holds promise to revolutionize drug discovery and therapeutics.
DNA Oligonucleotide Synthesis
Learn about the steps in phosphoramidite solid-phase method of Oligonucleotide Synthesis method that we use for DNA oligonucleotide manufacturing.
Modulated Gene Expression in 3D Cell Culture: Implications for Drug Discovery
Introduction of the modulation in expression profile for some genes and proteins when comparing 2D versus 3D cell culture conditions.
Lentiviral Transduction
Methods for Enhancing Lentiviral Transduction Efficiency
Phosphorothioate Oligonucleotides
Several phosphate backbone variants have been developed in an attempt to alter the chemical properties of native-state DNA and therefore overcome the two major challenges involved with using oligonucleotides in vivo.
Predesigned siRNA
We and Rosetta Inpharmatics, a recognized leader in Bioinformatics, have partnered to take RNAi research to the next level. Our exclusive siRNAdesign algorithm, licensed from Rosetta Inphamatics, has been optimized with over 3 years of continuous development for enhanced...
Reverse Transcription
One approach to the analysis of gene expression is to measure the concentration of mRNA of a gene. There are several challenges to such analyses, such as the differences in half life between different transcripts, the temporal patterns of transcription and...
Analysis of Fluorescent Dyes Used for Labeling DNA in Microarray Experiments
An article concerning Analyzing Properties of Fluorescent Dyes for Labeling DNA in Microarray Experiments presented by Sigma-Aldrich.com
Using Microarray Technology to Select Housekeeping Genes in Chinese Hamster Ovary Cells
In the present study, we have identifi ed species-specifi c housekeeping genes (HKGs) for Chinese Hamster Ovary (CHO) cells using data from microarray gene expression profiling.
pLKO-1 Vector & shRNA Design
Frequently asked questions about pLKO-1 vector & shRNA design
Small RNA and miRNA (microRNA) Sequencing
A review on reducing miRNA and other small RNAs sequencing bias with RealSeq®-AC RNA library preparation on the Illumina® RNA sequencing platform.
CRISPR-based Gene Activation
Sigma has developed a gene activation system based on a fusion of dCas9 to the catalytic histone acetyltransferase (HAT) core domain of the human E1A-associated protein p300.
shRNA Process and shRNA Diagram
What is shRNA, how does it work, and what is the process?
Retinoic Acid and Gene Expression
All-trans retinoic acid (RA, ATRA) is a pleiotropic activation factor that regulates genes associated with normal vertebrate cellular processes such as cell differentiation, cell proliferation, apoptosis, and embryonic development.
MISSION™ shRNA Library: Next Generation RNA Interference
MISSION™ shRNA Library: Next Generation RNA Interference
Tips for Cell Engineering using Cas9-GFP CRISPR plasmids
CRISPR endonucleases have shown wide variation in their activity, even among multiple CRISPRs designed within close genomic proximity.
Introduction to Cell Transfection
Transfection is the introduction of DNA, RNA, or proteins into eukaryotic cells and is used in research to study and modulate gene expression. Thus, transfection techniques and protocols serve as an analytical tool that facilitates the characterization of genetic functions...
Efficient Gene Silencing using MISSION® TRC shRNA Lentiviral Vectors
The RNAi Consortium (TRC) is a collaborative effort among several academic laboratories (including the Broad Institute, the Whitehead Institute for Biomedical Research, the Dana Farber Cancer Institute, Massachusetts General Hospital, Massachusetts Institute of Technology, Harvard Medical School, Columbia University, and...
Technical Bulletins
Technical bulletins for our shRNA products, including Bacterial Glycerol Stock, Purified Plasmid DNA Format, Lentiviral Format, MISSION® Control Vectors, MISSION Control Transduction Particles, MISSION Lentiviral Packaging Mix.
siRNA Design, Manufacturing, Transfection & Detection
RNA interference (RNAi) is a natural biological mechanism wherein short inhibitory RNA (siRNA) duplexes induce potent inhibition of gene expression.
siRNA Delivery in Mammalian Cell Lines
NA interference (RNAi) is a mechanism of posttranscriptional gene silencing effective in most eukaryotic organisms. The RNAi machinery is most likely an evolutionary conserved defense mechanism against retroviral assaults.
Ago RIP to Isolate microRNA and their Targets Using Imprint® RNA Immunoprecipitation Kit
Ago RIP to Isolate microRNA and their Targets Using Imprint® RNA Immunoprecipitation Kit
Utility of shRNA Clones
A complimentary approach to overexpression experiments is to observe the effects of F-Box Protein 6 (i.e. FBG2 or FBXO6) specific suppression on A1AT levels. A panel of FBG2 specific shRNA plasmids was transfected into COS-7 cells in equimolar amounts with...
TRC Library Citations
TRC 1.5 human and mouse shRNA libraries
Examination of Efficacy, Specificity, and Efficiency of an shRNA Lentiviral Library
The introduction of small interfering RNAs (siRNAs) into cultured cells provides a fast and efficient means of knocking down gene expression and has allowed siRNAs to quickly become a ubiquitous tool in molecular biology.
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