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Showing 1-25 of 25 results
hESC Transduction Protocol
A detailed protocol for the transduction of human embryonic stem cells using Lentiviral Vectors. Includes product list and references.
Genome Editing in Plants with CRISPR/Cas9
Zinc finger nucleases (ZFNs), were introduced by Sigma-Aldrich less than 8 years ago, but in that time the technology of targeted genome editing has advanced rapidly. Most recently, the discovery of the CRISPR/Cas9 pathway has accelerated interest in this field...
CRISPR Lentiviral Screening: Antibiotic Selection
You are not alone designing successful CRISPR, RNAi, and ORF experiments. Sigma-Aldrich was the first company to commercially offer lentivirus versions of targeted genome modification technologies and has the expertise and commitment to support new generations of scientists.
MystiCq® MicroRNA® Quantitation System
Method for purification, reverse transcription and quantitative PCR for MicroRNAs using Mysticq reagents
X-tremeGENE™ siRNA Transfection Reagent Protocol & Troubleshooting
X-tremeGENE™ siRNA Transfection Reagent Protocol & Troubleshooting
Escort™ IV Transfection Reagent Protocol
Escort™ IV Transfection Reagent Protocol
Suspension Stable Cells
General Protocol for Creation of Suspension Stable Cells Using MISSION Lentiviral Particles (SHVRS)
Lentivirus Protocols
MISSION Protocols in Functional Genomics and RNAi
Preparation of the Lentiviral Transduction Particles Using Packaging Plasmid Mix
Preparation of the Lentiviral Transduction Particles Using Packaging Plasmid Mix
Spinoculation Protocol
Complete protocol for Lentiviral transduction of suspension cells. This protocol describes the use of MISSION TRC shRNA Lentiviral Particles for long term silencing and phenotypic observation of suspension cells.
Lentiviral Transduction Protocol
Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.
Biodegradable Nanoparticles
Preparation for biodegradable nanoparticles and their use in transfection protocols .
Protocol for Transduction of Human Embryonic Stem Cells (hESCs) using Lentiviral Vectors
Protocol for Transduction of Human Embryonic Stem Cells (hESCs) using Lentiviral Vectors
CRISPR Cas 9 Nuclease RNA-guided Genome Editing
Learn about CRISPR Cas9, what it is and how it works. CRISPR is a new, affordable genome editing tool enabling access to genome editing for all.
RNAi In Situ
In Situ Hybridization of Whole-Mount Mouse Embryos with RNA Probes: Hybridization, Washes, and Histochemistry
Antibiotic Selection Optimization Using a Cytotoxicity Profile
A protocol describing how to use a cytotoxicity profile to select antibiotics.
X-tremeGENE™ 9 DNA Transfection Reagent Protocol
Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).
Protocol for Transducing Mouse Embryonic Fibroblasts (MEF) using MISSION® ExpressMag™ Super Magnetic Kit®
This detailed procedure allows you to transduce Mouse Embryonic Fibroblasts (MEF) using MISSION ExpressMag Super Magnetic Kit.
X-tremeGENE HP DNA Transfection Reagent Protocol
Cell preparation for transfection Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).
Preparation of Cationic Liposomes & Transfection of Cells - Avanti® Polar Lipids
This procedure describes the Preparation of Cationic Liposomes & Transfection of Cells
Integration of Sigma® TransPlex® WTA and the Complete WTA2 Kits with the Illumina Microarray Workflow
Amplification products generated by the TransPlex® WTA and Complete WTA2 kits are suitable for microarray target for expression analyses, and can be incorporated into existing Illumina workflows.
FACS Titration of Lentivirus Protocol
FACS (Fluorescence-Activated Cell Sorting) provides a method for sorting a mixed population of cells into two or more groups, one cell at a time, based on the specific light scattering and fluorescence of each cell. This method provides fast, objective...
MISSION® shRNA Guarantee
Upon purchase of the defined clone set quantity on the shRNA detail page for your gene of interest (this varies, but is typically 5 clones), we guarantee that at least one of those clones for a gene should yield greater...
Cancer Research Techniques
By focusing on analysis techniques and protocols specific for Cancer Research, Sigma-Aldrich is putting your research needs first. Protein Tyrosine Kinase and Phosphatase Expression Profiling in Cancer Research.