Applications
Content Type
Showing 1-14 of 14 results
Reproducibility with Biological Buffers
Biological buffers are organic substances that maintain a constant pH over a given range by neutralizing the effects of hydrogen ions.
Performing a purity and homogeneity check
This page shows how to perform a purification and homogeneity check of membrane proteins with products from GE Healthcare.
Precipitation Procedures
This page describes protein precipitation as an optional step in sample preparation for 2-D electrophoresis with GE Healthcare products.
DNA / Protein Electrophoresis and Troubleshooting Tables
DNA / Protein Electrophoresis and Troubleshooting Tables
Troubleshooting 2-D DIGE Results
Troubleshooting 2-D DIGE Results
Sample Preparation & Gel Electrophoresis Troubleshooting
The possible causes and potential remedies for challenges encountered during preparation of samples for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and optimizing electrophoresis conditions.
Fluorescent Multiplex Detection using Antibody Atto Dye Conjugates
Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.
Components of Rehydration Solution
This page describes components of rehydration solutions for use in 2-D Electrophoresis with GE Healthcare products.
Restriction Endonucleases - The Molecular Scissors
The term “Restriction enzyme” originated from the studies of Enterobacteria phage λ (lambda phage) in the laboratories of Werner Arber and Matthew Meselson.
Selecting Markers and Ladders for Gel Electrophoresis
Sigma-Aldrich offers a variety of markers for nucleic acid size determination of sample separated by various gel electrophoresis methods.
Reproducibility with Biological Buffers
Biological buffers are organic substances that maintain a constant pH over a given range by neutralizing the effects of hydrogen ions.
Protein Visualization
Prior to probing a membrane using precious antibodies, it is helpful to visualize the transferred proteins on the blot to ensure complete transfer and even loading. This page describes possible causes and potential remedies for challenges encountered during protein visualization.
Electrophoresis – Protein Staining Reagents
To meet the great diversity of protein analysis needs, Sigma offers a wide selection of protein visualization (staining) reagents. EZBlue™ and ProteoSilver™, designed specifically for proteomics, also perform impressively in traditional PAGE formats.
Detergent Properties and Applications
Use detergents more effectively in cell lysis, membrane protein and lipid solubilization, protein crystallization, or Western blotting.