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Enzymatic Assay of Proteinase K with Hemoglobin Substrate
Proteinase K (EC 3.4.21.64) activity can be measured spectrophotometrically using hemoglobin as the substrate. Proteinase K hydrolyzes hemoglobin denatured with urea, and liberates Folin-postive amino acids and peptides. One unit will hydrolyze hemoglobin to produce color equivalent to 1.0 μmol
Enzymatic Assay of Cathepsin B
This procedure applies to all products that have a specification for Cathepsin B activity determined by the liberation of 7-amino-4-methylcoumarin from Z-Arg-Arg 7-amido-4-methylcoumarin.
Enzymes for Alternative Energy Research
We are committed to help unlock the today’s challenge of releasing the energy of glucose that nature has cleverly locked into lignocellulosic biomass. To the untrained eye, starch and cellulose structures appear almost identical. Yet the barriers between cellulosic biomass
Phospholipase C
The hydrolysis of a minor membrane phospholipid, phosphatidylinositol 4,5-bisphosphate (PIP2) by a specific phospholipase C (PLC) is one of the earliest key events in the regulation of various cell functions by more than 100 extracellular signaling molecules.
Neuropeptidases
Sigma-Aldrich offers many products related to neuropeptidases for your research needs.
Enzyme Commission Numbers
Complete List of Enzyme Commission Numbers for metabolics research.
PKA & PKG
Our offers many products related to PKA & PKG for your research needs.
Tubulin
Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells.
Horseradish Peroxidase (HRP) Enzymes
Discover our peroxidase from horseradish enzymes, products, substrates, and inhibitors for your ELISA, immunoassay, and protein application needs.
Coenzyme A
Enzyme Reagent Coenzyme A (CoA, CoASH or HSCoA) is the key cofactor in first step of the TCA cycle, responsible for transferring the acetyl group from pyruvate oxidation to oxaloacetate yielding citrate. Available through Sigma-Aldrich online.
Complement Proteins
Application Index: Complement Proteins offered by Sigma-alderich online.The complement system is a complex cascade involving proteolytic cleavage of serum glycoproteins often activated by cell receptors. This cascade ultimately results in induction of the inflammatory response, phagocyte chemotaxis and opsonization, and
HMG-CoA Reductase (HMGR) Assay Kit
3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) is a transmembrane glycoprotein, located on the endoplasmic reticulum.
G Proteins (Heterotrimeric)
Heterotrimeric G proteins, comprising α, β and γ subunits, respond to extracellular signals generated by activated seven-transmembrane (7TM) receptors by modulating intracellular effector proteins such as enzymes and ion channels.
Fibrinogen/Fibrin
Fibrinogen is a ~340 kDa glycoprotein synthesised in the liver. It is a hexamer composed of pairs of three peptide chains (α, β, and γ). Fibrinogen is an acute phase protein that is part of the coagulation cascade of
Enzymatic Method for Determining Branched Chain Amino Acids (BCAA Assay)
Assay protocol for the colorimetric detection of Branched Chain Amino Acids (BCAA) in biological samples using the alanine assay kit.
β Secretase (BACE1) Activity Assay for BACE1 Inhibitor Screening
In recent years, the biological processes involved in the pathogenesis of Alzheimer’s disease (AD) have become increasingly better understood. It is now recognized that a key role is played by BACE1 proteases that cleave amyloid precursor protein (APP) to produce
Bioluminescent Firefly Luciferase Assays
Firefly luciferase is a widely used bioluminescent reporter for studying gene regulation and function. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues.
Enzymatic Assay of Chitosanase from Streptomyces Species
Enzymatic Assay of Chitosanase from Streptomyces Species
Nuclear Receptors (PPARs)
Peroxisome proliferator activated receptors (PPARs) are members of the nuclear hormone receptor superfamily of ligand-activated transcription factors that are related to retinoid, steroid and thyroid hormone receptors.
Aprotinin
While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.
Enzymatic Method for Determining Amylase Activity (Amylase Activity Assay)
Assay protocol for the colorimetricdetection of amylase activity in biological samples using the Amylase Activity assay kit.
Decorin
Decorin is a small proteoglycan, which has been found associated with collagen fibrils in all connective tissues. In the skeletal system it is found primarily in the extracellular matrix of articular cartilage, which covers the surface of adult bones in
Protein Kinase C (PKC)
Protein kinase C (PKC) is an AGC kinase that phosphorylates serine and threonine residues in many target proteins.
Cholesterol Esterification
Cholesterol undergoes esterification to improve transport. Cholesterol esters are more easily packaged into the interior of lipoproteins - increasing the quantity that can be readily transported in the blood stream.
Neurotensin Receptors
Neurotensin is a 13 amino acid regulatory peptide found mainly in gut and brain. Intestinal neurotensin is produced by a discrete population of endocrine cells (N cells) scattered throughout the jejuno-ileal mucosa.
Mucin
Mucins are a diverse family of densely glycosylated proteins. Mucin domains within the protein core are rich in threonine, serine and hydroxyproline enabling post-translational O-glycosylation.
Phospholipase D
Phosphatidylcholine (PC)-specific phospholipase D (PLD) enzymes catalyze the hydrolysis of PC to generate choline and phosphatidic acid. These enzymes belong to a superfamily of phosphatidyltransferases with a characteristic unique duplicated catalytic domain motif, the PLD or " HKD"
How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions
Instructions for working with enzymes supplied as ammonium sulfate suspensions
Drug Conjugate Analysis using β-Glucuronidases
Drug conjugate analysis and the enzymatic hydrolysis of glucuronides
Phosphodiesterases
Cyclic nucleotide phosphodiesterases (PDEs) catalyze the hydrolysis of cAMP and/or cGMP. There are 11 different mammalian PDE families.
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