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IFN-γ ELISpot Assays on MultiScreen® IP
The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level.
Enzymatic Assay of Phosphoglucomutase (EC 5.4.2.2)
This procedure may be used for all Phosphoglucomutase products except for Phosphoglucomutase, Catalog Number P4109.
Enzymatic Assay of Neuraminidase
Enzymatic Assay of Neuraminidase applies to products that have a specification for neuraminidase content by enzymatic determination.
Enzymatic Assay of Protease Using Casein As a Substrate
To standardize a procedure for the enzymatic assay of Protease using Casein as a substrate.
Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)
Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)
Assay Procedure for Glycerol Dehydrogenase
Assay Procedure for Glycerol Dehydrogenase
Alkaline Phosphatase (AP) Protocol
Alkaline phosphatase (AP) is a non-specific phosphomonoester hydrolase that catalyzes the hydrolysis of a wide variety of organic monophosphates.
LPL Activity Assay Protocol
Lipoprotein lipase (LPL) hydrolyzes triglycerides associated with VLDL.
LCAT Activity Assay Kit
The LCAT Activity Assay Kit is a fluorometric assay useful for measuring phospholipase activity of LCAT.
Enzymatic Assay of Phytase (EC 3.1.3.8)
Derived from procedure SSPHYT02. Includes template updates to current SOP specifications and incorporation of notes into the procedure.
Assay Procedure for Protease
This procedure is for informational purposes including assay Procedure, definition, and calculations for Protease
Enzymatic Assay of Hyaluronidase (3.2.1.35)
This procedure may be used for Hyaluronidase products.
Enzymatic Assay of Superoxide Dismutase
Enzymatic Assay of Superoxide Dismutase
Pull-down assays
This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.
Enzymatic Assay of Pectinase
Objective: To standardize a procedure for determining the enzymatic activity of Pectinase.
Enzymatic Method for Determining Urea (Phenylalanine Assay)
Assay protocol for the colorimetric detection of urea in biological samples using the urea assay kit.
Enzymatic Assay of Laccase (EC 1.10.3.2)
Laccase (EC 1.10.3.2) is a cuproenzyme that oxidizes various types of phenols and similar aromatic compounds aromatic amines with the reduction of molecular oxygen to water, therefore, is used as a biocatalyst.
Enzymatic Assay of Trypsin Inhibitor
This technical article described the Enzymatic Assay of Trypsin Inhibitor.
Enzymatic Assay of Lyticase
This procedure may be used for the determination of Lyticase activity using Baker’s yeast as the substrate.
Enzymatic Assay of Alcohol Dehydrogenase (EC 1.1.1.1)
This procedure may be used for Alcohol Dehydrogenase products.
Enzymatic Assay of Ficin (EC 3.4.22.3)
This procedure may be used for all Ficin products.
Enzymatic Assay of α-Glucosidase by the Modified Boehenger Procedure (EC 3.2.1.20)
Enzymatic Assay of α-Glucosidase by the Modified Boehenger Procedure (EC 3.2.1.20)
Enzymatic Assay of Endoproteinase Glu-C (EC 3.4.21.19)
This procedure may be used for determination of Endoproteinase Glu-C enzymatic activity using N-t-Boc-L-glutamic acid α-phenyl ester as the substrate.
Procedure for Enzymatic Assay of Trypsin (EC 3.4.21.4)
This procedure is for products with a specification for Trypsin activity using Na-Benzoyl-L-arginine ethyl ester (BAEE) as a substrate. The procedure is a continuous spectrophotometric rate determination (A253, Light path = 1 cm).
Enzymatic Assay of 3α-Hydroxysteroid Dehydrogenase with Androsterone (EC 1.1.1.50)
Enzymatic Assay of 3α-Hydroxysteroid Dehydrogenase with Androsterone (EC 1.1.1.50)
Enzymatic Assay of Lysozyme (EC 3.2.1.17)
This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.
Enzymatic Assay of Cellulase
To standardize an enzymatic assay procedure of cellulase.
Enzymatic Assay of Achromopeptidase
Enzymatic Assay of Achromopeptidase
Enzymatic Assay of Plasmin with D-Val-Leu-Lys-p-Nitroanilide Dihydrochloride
Enzymatic Assay of Plasmin with D-Val-Leu-Lys-p-Nitroanilide Dihydrochloride
Enzymatic Assay of Amyloglucosidase (EC 3.2.1.3)
This procedure may be used for the determination of Amyloglucosidase activity using starch as the substrate.
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