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Jonathan Michaud-Levesque et al.
The Journal of biological chemistry, 284(32), 21338-21346 (2009-06-11)
Protein arginine methyltransferase 6 (PRMT6) is known to catalyze the generation of asymmetric dimethylarginine in polypeptides. Although the cellular role of PRMT6 is not well understood, it has been implicated in human immunodeficiency virus pathogenesis, DNA repair, and transcriptional regulation.
Birgit Ungerböck et al.
Lab on a chip, 13(8), 1593-1601 (2013-02-28)
In this work we present a high resolution oxygen imaging approach, which can be used to study 2D oxygen distribution inside microfluidic environments. The presented setup comprises a fabrication process of microfluidic chips with integrated luminescent sensing films combined with
Nagat Areid et al.
Journal of materials science. Materials in medicine, 29(5), 56-56 (2018-05-08)
The purpose of this study was to evaluate blood and platelet response to nanostructured TiO2 coatings and to investigate the effect of Ultraviolet (UV) light treatment on blood clotting ability, platelet activation and protein adhesion. Ti-6Al-4V titanium alloy plates (n = 138)
Michelle Richner et al.
Nature protocols, 10(10), 1543-1555 (2015-09-18)
The ability to generate human neurons of specific subtypes of clinical importance offers experimental platforms that may be instrumental for disease modeling. We recently published a study demonstrating the use of neuronal microRNAs (miRNAs) and transcription factors to directly convert
Lan Jin et al.
Experimental biology and medicine (Maywood, N.J.), 233(7), 849-859 (2008-05-01)
CEACAM1-4L (carcinoembryonic antigen cell adhesion molecule 1, with 4 extracellular Ig-like domains and a long, 71 amino acid cytoplasmic domain) is expressed in epithelial cells and activated T-cells, but is down-regulated in most epithelial cell cancers and T-cell leukemias. A
Kristina Woodruff et al.
Scientific reports, 6, 23937-23937 (2016-04-01)
Mammalian synthetic biology could be augmented through the development of high-throughput microfluidic systems that integrate cellular transfection, culturing, and imaging. We created a microfluidic chip that cultures cells and implements 280 independent transfections at up to 99% efficiency. The chip
Michelle A Digman et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(7), 2170-2175 (2009-01-27)
We describe a method to detect molecular complexes and measure their stoichiometry in living cells from simultaneous fluctuations of the fluorescence intensity in two image channels, each detecting a different kind of protein. The number and brightness (N&B) analysis, namely
Emily C Benesh et al.
Molecular biology of the cell, 24(22), 3496-3510 (2013-09-21)
Directional cell movement is universally required for tissue morphogenesis. Although it is known that cell/matrix interactions are essential for directional movement in heart development, the mechanisms governing these interactions require elucidation. Here we demonstrate that a novel protein/protein interaction between
Jimmy Y C Chow et al.
American journal of physiology. Gastrointestinal and liver physiology, 294(4), G899-G905 (2008-02-02)
Transforming growth factor-beta (TGF-beta) suppresses growth via the TGF-beta-SMAD pathway but promotes growth in cancer cells with disrupted SMAD signaling and corresponds to an invasive phenotype. TGF-beta also downregulates the tumor suppressor PTEN that is rarely mutated in sporadic pancreatic
Michelle A Digman et al.
Biophysical journal, 96(2), 707-716 (2009-01-27)
We describe a general method for detecting molecular complexes based on the analysis of single molecule fluorescence fluctuations from laser scanning confocal images. The method detects and quantifies complexes of two different fluorescent proteins noninvasively in living cells. Because in
Lucia Pulzova et al.
Scientific reports, 1, 86-86 (2012-02-23)
Lyme borreliosis is the most widespread vector-borne disease in temperate zones of Europe and North America. Although the infection is treatable, the symptoms are often overlooked resulting in infection of the neuronal system. In this work we uncover the underlying
Vincent van Duinen et al.
International journal of molecular sciences, 21(13) (2020-07-11)
To advance pre-clinical vascular drug research, in vitro assays are needed that closely mimic the process of angiogenesis in vivo. Such assays should combine physiological relevant culture conditions with robustness and scalability to enable drug screening. We developed a perfused
Partial primary structure of bovine plasma fibronectin: three types of internal homology
Petersen TE, et al.
Proceedings of the National Academy of Sciences of the USA, 80(1), 137-141 (1983)
Joanne Nolan et al.
Gene, 761, 145024-145024 (2020-08-07)
Understanding how various pathologies of breast cancer respond to their environment may be imperative in the creation of novel therapeutic targets. Central to the organisation and behaviour of cells within the tumour microenvironment is the extracellular matrix (ECM), a meshwork
Jeanette C Perron et al.
Neural development, 6, 36-36 (2011-11-17)
Bone morphogenetic protein (BMP)7 evokes both inductive and axon orienting responses in dorsal interneurons (dI neurons) in the developing spinal cord. These events occur sequentially during the development of spinal neurons but in these and other cell types such inductive
Hsin-Yi Yang et al.
BMC musculoskeletal disorders, 15, 173-173 (2014-06-03)
Knee osteoarthritis (OA) is a complex disease involving both biomechanical and metabolic factors that alter the tissue homeostasis of articular cartilage and subchondral bone. The catabolic activities of extracellular matrix degradation products, especially fibronectin (FN), have been implicated in mediating
Karukriti Kaushik Ghosh et al.
Infection and immunity, 87(12) (2019-09-25)
Leptospirosis is one of the most widespread zoonoses caused by pathogenic Leptospira spp. In this study, we report that the LIC11966/ErpY-like lipoprotein is a surface-exposed outer membrane protein exclusively present in pathogenic species of Leptospira The recombinant ErpY (rErpY)-like protein
Fang Liu et al.
Neoplasia (New York, N.Y.), 10(11), 1275-1284 (2008-10-28)
Accumulating evidence suggests that multiple complex mechanisms may be involved, simultaneously or complementarily, in the emergence and development of multidrug resistance (MDR) in various cancers. Cell adhesion-mediated MDR is one such mechanism. In the present study, we initially observed increased
Marianne J van den Heuvel et al.
Journal of virology, 79(13), 8164-8170 (2005-06-16)
Bovine leukemia virus (BLV) is a deltaretrovirus that infects cattle worldwide. In agriculturally intensive regions, approximately 30% of dairy cows are BLV infected. Like the human T-cell leukemia virus (HTLV), there is a lengthy period of viral quiescence after initial
Ritsuya Niwayama et al.
Developmental cell, 51(5), 564-574 (2019-11-19)
Oriented cell division patterns tissues by modulating cell position and fate. While cell geometry, junctions, cortical tension, and polarity are known to control division orientation, relatively little is known about how these are coordinated to ensure robust patterning. Here, we
Toru Yoshihara et al.
PLoS genetics, 14(8), e1007545-e1007545 (2018-08-17)
It is uncertain which β4-galactosyltransferase (β4GalT; gene name, B4galt), β4GalT-5 and/or β4GalT-6, is responsible for the production of lactosylceramide (LacCer) synthase, which functions in the initial step of ganglioside biosynthesis. Here, we generated conditional B4galt5 knockout (B4galt5 cKO) mice, using
M Bernhardt et al.
Nature communications, 9(1), 3641-3641 (2018-09-09)
We present a correlative microscopy approach for biology based on holographic X-ray imaging, X-ray scanning diffraction, and stimulated emission depletion (STED) microscopy. All modalities are combined into the same synchrotron endstation. In this way, labeled and unlabeled structures in cells
Matthew J Stroud et al.
The Journal of biological chemistry, 286(28), 24987-24995 (2011-05-13)
The microtubule (MT) and actin cytoskeletons are fundamental to cell integrity, because they control a host of cellular activities, including cell division, growth, polarization, and migration. Proteins involved in mediating the cross-talk between MT and actin cytoskeletons are key to
Magali Berthouze et al.
The EMBO journal, 28(12), 1684-1696 (2009-05-09)
Agonist-induced ubiquitination of the beta(2) adrenergic receptor (beta(2)AR) functions as an important post-translational modification to sort internalized receptors to the lysosomes for degradation. We now show that this ubiquitination is reversed by two deubiquitinating enzymes, ubiquitin-specific proteases (USPs) 20 and
Belén Sánchez-Maldonado et al.
Stem cells and development, 27(22), 1557-1576 (2018-09-27)
Cell spheroids are inducible or spontaneously generated cell aggregates produced in vitro that can provide a valuable model for developmental biology, stem cell biology, and cancer therapy research. This investigation aimed to define the cellular identity of spheroids spontaneously generated
Nicolás E Muzzio et al.
Biomaterials science, 6(8), 2230-2247 (2018-07-07)
The modulation of cell adhesion via biologically inspired materials plays a key role in the development of realistic platforms to envisage not only mechanistic descriptions of many physiological and pathological processes but also new biointerfacial designs compatible with the requirements
Alexei Poliakov et al.
The Journal of cell biology, 183(5), 933-947 (2008-12-03)
In this study, we investigated whether the ability of Eph receptor signaling to mediate cell repulsion is antagonized by fibroblast growth factor receptor (FGFR) activation that can promote cell invasion. We find that activation of FGFR1 in EphB2-expressing cells prevents
Ita Costello et al.
BMC developmental biology, 9, 54-54 (2009-10-24)
Smad4 mutant embryos arrest shortly after implantation and display a characteristic shortened proximodistal axis, a significantly reduced epiblast, as well as a thickened visceral endoderm layer. Conditional rescue experiments demonstrate that bypassing the primary requirement for Smad4 in the extra-embryonic
R W Redline et al.
The Journal of clinical investigation, 85(6), 1951-1958 (1990-06-01)
Because of their paternal antigens, the fetus and placenta may be considered an allograft in the maternal host. Local properties of the maternal-fetal interface, the placenta and decidua basalis, are important in preventing maternal immunologic rejection of the fetoplacental allograft.
Lillian K Fritz-Laylin et al.
The Journal of cell biology, 216(6), 1673-1688 (2017-05-06)
Diverse eukaryotic cells crawl through complex environments using distinct modes of migration. To understand the underlying mechanisms and their evolutionary relationships, we must define each mode and identify its phenotypic and molecular markers. In this study, we focus on a
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