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Jae Ho Choi et al.
Journal of agricultural and food chemistry, 63(5), 1468-1476 (2015-01-16)
We investigated the inhibitory effects of Platycodon grandiflorum root-derived saponins (Changkil saponins: CKS) on ovalbumin-induced airway inflammation in mice. CKS suppressed leukocytes number, IgE, Th1/Th2 cytokines, and MCP-1 chemokine secretion in bronchoalveolar lavage fluid. Also, ovalbumin-increased MUC5AC, MMP-2/9, and TIMP-1/-2
M M Reis et al.
Animal : an international journal of animal bioscience, 9(9), 1500-1508 (2015-05-21)
This experiment compared growth, physiological, and reproductive responses of beef heifers with (MI) or without (CON) access to a creep-feeder, as a manner to stimulate metabolic imprinting while nursing their dams. On day 0, 60 Angus × Hereford heifers were
Matthias Klemm et al.
PloS one, 10(7), e0131640-e0131640 (2015-07-21)
Fluorescence lifetime imaging ophthalmoscopy (FLIO) is a new technique for measuring the in vivo autofluorescence intensity decays generated by endogenous fluorophores in the ocular fundus. Here, we present a software package called FLIM eXplorer (FLIMX) for analyzing FLIO data. Specifically
Savitri Krishnamurthy et al.
Archives of pathology & laboratory medicine, 137(12), 1733-1739 (2013-08-21)
Whole slide imaging (WSI) is now used for educational purposes, for consultation, and for archiving and quantitation of immunostains. However, it is not routinely used for the primary diagnosis of hematoxylin-eosin-stained tissue sections. To compare WSI using the Aperio digital
L S Herculano et al.
The journal of physical chemistry. B, 117(6), 1932-1937 (2013-02-05)
Eosin Y is known to be a powerful probe of biological molecules and an efficient photosensitizing agent for the production of singlet molecular oxygen. Under continuous laser excitation, degradation through photobleaching is observed in aqueous solutions of eosin Y; this
J Filipowska et al.
Biomedical materials (Bristol, England), 9(6), 065001-065001 (2014-10-21)
In this study, 3D porous bioactive composite scaffolds were produced and evaluated for their physico-chemical and biological properties. Polymer poly-L-lactide-co-glycolide (PLGA) matrix scaffolds were modified with sol-gel-derived bioactive glasses (SBGs) of CaO-SiO2-P2O5 systems. We hypothesized that SBG incorporation into PLGA
F Gani et al.
SADJ : journal of the South African Dental Association = tydskrif van die Suid-Afrikaanse Tandheelkundige Vereniging, 67(7), 370-373 (2013-08-21)
Orthokeratinised jaw cyst (OJC) is an entity distinct from odontogenic keratocyst (OKC) that has not been fully characterised at the molecular level. To compare the proliferative activity of the epithelial linings of OKC and OJC immunohistochemically, using Ki-67 and cyclin
Patrick T Brown et al.
Cell and tissue research, 358(1), 149-164 (2014-06-15)
Embryonic stem cells (ESCs) and mesenchymal stem cells (MSCs) have been studied for years as primary cell sources for regenerative biology and medicine. MSCs have been derived from cell and tissue sources, such as bone marrow (BM), and more recently
Maria Q Gaunsbaek et al.
ORL; journal for oto-rhino-laryngology and its related specialties, 76(5), 288-301 (2014-12-17)
To investigate the presence of surfactant protein (SP) A, B, C and D in nasal airways and to determine whether the proteins exert their main functions in nasal secretions or in the deeper layers of the nasal mucosa. Volunteers were
Ji-Eun Baek et al.
Biochemical and biophysical research communications, 451(3), 442-448 (2014-08-12)
The transcription factors, Runx2 and Osterix (Osx), act downstream in the BMP2 pathway, and they are essential for osteoblast differentiation and bone formation. While Runx2 expression is normal in Osx-null mice, Osx is not expressed in Runx2-null mice, indicating that
Jason K F Wong et al.
PloS one, 9(11), e112672-e112672 (2014-11-11)
Repaired tendons may be complicated by progressive fibrosis, causing adhesion formation or tendon softening leading to tendon rupture and subsequent reduced range of motion. There are few therapies available which improve the gliding of damaged tendons in the hand. We
Brent P Goodman et al.
Journal of clinical neuromuscular disease, 14(2), 78-81 (2012-11-23)
Inclusion body myositis is the most common idiopathic inflammatory myopathy in elderly individuals. It typically causes proximal and distal limb weakness with forearm flexors and quadriceps being the most severely affected muscles. Axial musculature is infrequently involved. Here, we report
Radoslaw Kwapiszewski et al.
Analytical and bioanalytical chemistry, 406(18), 4551-4556 (2014-05-13)
Interest in the microfluidic environment, owing to its unique physical properties, is increasing in much innovative chemical, biological, or medicinal research. The possibility of exploiting and using new phenomena makes the microscale a powerful tool to improve currently used macroscopic
Swati Mishra et al.
Journal of biomedical materials research. Part A, 103(10), 3237-3249 (2015-03-18)
Three-dimensional (3D) patterning and engineering of biomaterials and biointerfaces have helped bioengineers harness the full potential of cell immobilization for different biomedical applications. However, the bioengineering of an efficient cell immobilized tool, having application in cell biology and tissue engineering
Hubert Pausch et al.
PLoS genetics, 10(1), e1004044-e1004044 (2014-01-07)
Genetic variants underlying reduced male reproductive performance have been identified in humans and model organisms, most of them compromising semen quality. Occasionally, male fertility is severely compromised although semen analysis remains without any apparent pathological findings (i.e., idiopathic subfertility). Artificial
Leah L Zagore et al.
Molecular and cellular biology, 35(23), 4030-4042 (2015-09-24)
RNA binding proteins (RBPs) are increasingly recognized as essential factors in tissue development and homeostasis. The polypyrimidine tract binding (PTB) protein family of RBPs are important posttranscriptional regulators of gene expression. In the nervous system, the function and importance of
Seong-Jin Yu et al.
PloS one, 10(7), e0132487-e0132487 (2015-07-18)
Recent studies have indicated that type 2 cannabinoid receptor (CB2R) agonists reduce neurodegeneration after brain injury through anti-inflammatory activity. The purpose of this study was to examine the time-dependent interaction of CB2R and inflammation in stroke brain. Adult male rats
Chang Hoon Moon et al.
Investigational new drugs, 32(3), 400-411 (2013-11-10)
CKD-516 is a benzophenone analog in which the B ring is modified by replacement with a carbonyl group. The study assessed CKD-516 as a vascular disrupting agent or anti-cancer drug. To assess the effect of S516 on vascularization, we analyzed
K F Wong et al.
American journal of clinical pathology, 141(2), 280-284 (2014-01-18)
To report the demonstration of double minutes with MYC amplification in a case of myeloproliferative neoplasm with monocytosis in transformation by a combination of standard karyotyping and interphase and metaphase fluorescence in situ hybridization (FISH). To determine the lineage involvement
Zheng Cheng et al.
PloS one, 9(11), e112719-e112719 (2014-11-15)
Activation of the cholinergic anti-inflammatory pathway, which relies on the α7nAchR (alpha 7 nicotinic acetylcholine receptor), has been shown to decrease proinflammatory cytokines. This relieves inflammatory responses and improves the prognosis of patients with experimental sepsis, endotoxemia, ischemia/reperfusion injury, hemorrhagic
Kathy Saraiva-Pava et al.
World journal of gastroenterology, 21(21), 6526-6542 (2015-06-16)
To establish a cellular model correctly mimicking the gastric epithelium to overcome the limitation in the study of Helicobacter pylori (H. pylori) infection. Aiming to overcome this limitation, clones of the heterogenic cancer-derived NCI-N87 cell line were isolated, by stably-transducing
Julian C Lui et al.
Pediatric research, 77(3), 406-415 (2014-12-19)
In juvenile mammals, the epiphyses of long bones grow by chondrogenesis within the articular cartilage. A better understanding of the molecular mechanisms that regulate the growth of articular cartilage may give insight into the antecedents of joint disease, such as
R Bryan Bell et al.
Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons, 71(7), 1268-1277 (2013-03-05)
The purpose of this study was to assess the predictability of sentinel lymph node biopsy (SNB) for oral squamous cell carcinoma (OSCC) when pathologic processing is performed without serial step sectioning. We prospectively enrolled 36 patients with T1 or T2
Nina-Fee Hübner et al.
Aesthetic plastic surgery, 38(5), 976-984 (2014-07-13)
This preliminary ex vivo study aimed to clarify the pathophysiologic mechanisms of fat tissue depletion by subcutaneous drug application. Therefore, the lipolytic effects of phosphatidylcholine plus deoxycholate (Lipostabil) (L) and of deoxycholate (DC) alone were compared with those of sodium
Jianyun Dong et al.
Chemical research in toxicology, 28(3), 518-531 (2015-01-31)
1,2:3,4-Diepoxybutane (DEB) is a major carcinogenic metabolite of 1,3-butadiene (BD), which has been shown to cause DNA strand breaks in cells through its potential genotoxicity. The adverse effect of DEB on male reproductive cells in response to DNA damage has
Y Chen et al.
Andrologia, 47(6), 694-699 (2014-08-02)
Cryotop is a carrier that has been used successfully in the cryopreservation of human spermatozoa. Here, we explored a novel method to vitrify human spermatozoa without cryoprotective agents (CPAs) using Cryotop. Spermatozoa from 21 Normozoospermic patients were collected and vitrified
Mihaela Necula et al.
The Journal of biological chemistry, 282(14), 10311-10324 (2007-02-08)
Alzheimer disease is characterized by the abnormal aggregation of amyloid beta peptide into extracellular fibrillar deposits known as amyloid plaques. Soluble oligomers have been observed at early time points preceding fibril formation, and these oligomers have been implicated as the
Yevgen Chornenkyy et al.
Molecular cancer therapeutics, 14(11), 2560-2568 (2015-09-10)
Pediatric high-grade astrocytomas (pHGA) and diffuse intrinsic pontine gliomas (DIPG) are devastating malignancies for which no effective therapies exist. We investigated the therapeutic potential of PARP1 inhibition in preclinical models of pHGA and DIPG. PARP1 levels were characterized in pHGA
A comparison of lyophilized amniotic membrane with cryopreserved amniotic membrane for the reconstruction of rabbit corneal epithelium.
Ahn J, et al.
Biotechnology and Bioprocess Engineering: BBE, 10, 262-269 (2005)
Pascal Stuelsatz et al.
Developmental biology, 397(1), 31-44 (2014-09-23)
Extraocular muscles (EOMs) are highly specialized skeletal muscles that originate from the head mesoderm and control eye movements. EOMs are uniquely spared in Duchenne muscular dystrophy and animal models of dystrophin deficiency. Specific traits of myogenic progenitors may be determinants
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