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End Point PCR Protocol for Long and Accurate DNA Amplification
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.
Long and Accurate PCR Amplification of DNA (D8045)
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. AccuTaq LA.
Low Contaminant Amplification of DNA Using MTP™ Taq DNA Polymerase
Method for bacterial genome analysis and detection of pathogens. Minimize false positive PCRs through lab design and reagents tested for use in bacterial PCR applications.
Amplification of DNA Using Jumpstart™ Taq DNA Polymerase (D4184)
Hot Start Taq Polymerase protocol to reduce non-specific amplification, with MgCl2 Optimization
Amplification of DNA Using Jumpstart™ REDTaq® DNA Polymerase (D8187)
Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
Long and Accurate PCR Amplification of DNA with RedAccuTaq® (D4812)
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. Red dye allows direct loading of reaction on a gel. REDAccuTaq LA
Amplification of Damaged DNA with Restorase DNA Polymerase (R1028)
Method for amplification of DNA from damaged DNA sources. Particularly useful for DNA extracted from old samples.
Standard PCR Protocol
Learn standard PCR protocol steps and review reagent lists or cycling parameters. This method for routine PCR amplification of DNA uses standard Taq DNA polymerase.
Amplification of Genomic DNA using REDTaq DNA Polymerase
Method for PCR using genomic DNA or other complex templates
End-Point PCR: Antibody-Mediated Hot Start PCR Protocol with Enhanced Specificity and Yield
Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
Amplification of DNA Using Jumpstart™ Taq DNA Polymerase (D9307)
Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
Method for PCR amplification of Bacterial DNA with low contamination using MTP™ Taq DNA Polymerase (D7442)
Method for PCR amplification of Bacterial DNA with low contamination using MTP™ Taq DNA Polymerase (D7442)
Preactivated Magnetic Beads for Affinity Chromatography
Covalent coupling of antibodies, aptamers, and proteins with a NHS Mag Sepharose, Sera-Mag Carboxylate-Modified, Sera-Mag SpeedBeads Carboxylate-Modified from Cytiva.
Enzymatic Method for Determining Alanine (Alanine Assay)
Assay protocol for the colorimetric/fluorometric detection of Alanine in biological samples using the alanine assay kit.
dNTP Mediated Hot Start PCR Protocol
Hot Start dNTPs are modified with a thermolabile protecting group at the 3’ terminus. The presence of this modification blocks nucleotide incorporation by DNA polymerase until the nucleotide protecting group is removed during a heat activation step.
MTP Activity Assay Kit Protocol
The MTP Activity Assay Kit is a sensitive, homogeneous fluorometric assay useful for continuous measurement of MTP activity in cell lysate or tissue homogenate.
Enzymatic Method for Determining Glucose-6-Phosphate (Glucose-6-Phosphate Assay)
Assay protocol for the colorimetric detection of glucose-6-phosphate in biological samples using the glucose-6-phosphate assay kit.
Enzymatic Method for Determining Phenylalanine (Phenylalanine Assay)
Assay protocol for the fluorometric detection of Phenylalanine in biological samples using the phenylalanine assay kit.
Alkaline Phosphatase Protocol
Alkaline phosphatase (AP) is a non-specific phosphomonoester hydrolase that catalyzes the hydrolysis of a wide variety of organic monophosphates.
Enzymatic Method for Determining Galactose (Galactose Assay)
This assay protocol is suitable for the colorimetric/fluorometric detection of Galactose and Lactose in cell and tissue culture supernatants, urine, plasma, serum, and other biological samples using the Galactose Assay Kit.
Heavy Metals in Textiles
Sample preparation and determination of heavy metals in textiles.
Purification or Removal of Biotin and Biotinylated Substances
This page shows how to purify or remove biotin and biotinylated substances with Streptavidin Sepharose High Performance from Cytiva.
Enzymatic Assay of Choloylglycine Hydrolase
To standardize a procedure for the determination of the enzymatic assay of choloylglycine hydrolase.
Protease Inhibition Using Protease Inhibitor Mix
Protease Inhibitor Mix from Cytiva inhibits serine, cysteine, metalloproteases, and calpain proteases and is suitable for animal and plant tissues, yeast, and bacteria.
Alkaline Phosphatase (AP) Protocol
Alkaline phosphatase (AP) is a non-specific phosphomonoester hydrolase that catalyzes the hydrolysis of a wide variety of organic monophosphates.
Enzymatic Method for Determining D-Sorbitol (D-Sorbitol Assay)
Assay protocol for the colorimetric detection of D-Sorbitol in samples using the D-Sorbitol assay kit.
Enzymatic Assay of Proteinase K (EC 3.4.21.64)
Proteinase K (EC 3.4.21.64) activity can be measured spectrophotometrically using hemoglobin as the substrate. Proteinase K hydrolyzes hemoglobin denatured with urea, and liberates Folin-postive amino acids and peptides. One unit will hydrolyze hemoglobin to produce color equivalent to 1.0 μmol
Determination of Fipronil and Fipronil Sulfone in Food Using Purospher® STAR RP-18 Endcapped Column and UV Detection
broad spectrum insecticide, egradation product, phenylpyrazole pesticide, World Health Organization (WHO), HPLC, UV, testing of Fipronil and Fipronil sulfone in Food
Enzymatic Galactose and Lactose Assay Protocol
This assay protocol is suitable for the colorimetric/fluorometric detection of Galactose and Lactose in cell and tissue culture supernatants, urine, plasma, serum, and other biological samples using the Galactose and Lactose Assay Kit (MAK011). In this assay kit, galactose is
Enzymatic Assay of 5’-Nucleotidase
Objective: To standardize a procedure for determining the enzymatic activity of 5’-Nucleotidase.
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