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In Vitro Differentiation of Human iPS Cells Into Colon Organoids in Serum-Free Cell Culture Conditions
Highly characterized cryopreserved human colonic organoids and a step-by-step organoid culture protocol for epithelial intestinal organoid differentiation from iPS cells.
Duolink® PLA Multicolor Detection Protocol
Duolink® PLA Multicolor Detection Protocol
Prestige Antibody® Immunofluorescence Procedure
We presents a protocol for Immunofluorescence Procedure. A large number of the Prestige Antibodies have been used in subcellular localization studies by immunofluorescence (IF) staining of three cell lines: A-431, U-2 OS, and U-251MG.
Fluorescent in situ Hybridization (FISH)
Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.
Transfection
Transfection reagents, protocols, and resources including Roche X-tremeGENE transfection reagents for DNA, siRNA, miRNA, and CRISPR/Cas9 gene editing components.
Duolink® PLA Fluorescence Protocol
Protocol for immunofluorescent detection of proteins in cells and tissue
3D Imaging of Optically Cleared Spheroids in Corning® Spheroid Microplates
Discover how Corning® Spheroid Microplates played a key role in the use of use of 3D cell cultures for in vitro drug discovery assay.
Tips for Optimizing Immunofluorescence Protocols to Get the Best Image
Uses for immunofluorescence (IF)—where an antibody conjugated to a molecule that fluoresces when excited by lasers— include protein localization, confirmation of post-translational modification or activation, and proximity to/complexing with other proteins.
Cell Cycle Analysis Using a Nucleoside Triphosphate (NTP) Transporter Molecule for Rapid DNA Labeling in Living Cells
Regulation of the cell cycle involves processes crucial to the survival of a cell, including the detection and repair of genetic damage as well as the prevention of uncontrolled cell division associated with cancer. The cell cycle is a four-stage
Fast and Highly Specific Histological Detection of Pathogenic Fungi in Human Tissue
The recently developed Atto-dye labeled lectins have many applications, including carbohydrate, mitogenic and histochemical studies.
Detection of Protein-protein Interactions by in situ PLA
Proteins are complex biological molecules essential for cellular structure and functions. The majority of proteins commonly interact with various molecules, including other proteins, in order to exert their functions.
Prestige Antibodies® Powered by Atlas Antibodies in Immunofluorescence Applications
One major rationale for investigating the subcellular location of a specific protein is that location is often tightly connected to function. For example, proteins locating to the nucleus are frequently implicated in gene regulation, proteins in mitochondria with energy production
Fluorescent Live Cell Imaging of LC3 Mediated Autophagy using LentiBrite™ Lentiviral Biosensors
High titer lentiviral particles for LC3 variants used for live cell analysis of cellular autophagy.
How Proximity Ligation Assays (PLA) Work
Learn how Proximity Ligation Assay technology works and how the protein-protein interaction control kit can confirm in situ detection of EGF-induced EGFR-HER2 dimerization.
Protocol Guide: Immunofluorescent Staining of Whole-Mount Organoids using Antibodies
How to stain organoids? A complete step-by-step protocol for immunofluorescent (IF) and immunocytochemical (ICC) staining of organoid cultures using antibodies
Visualize your CRISPR Experiment with MISSION
Watch Cas9-GFP proteins work inside your cells and deliver the powerful nuclease editing you have come to trust and expect. MISSION™ SpCas9-GFP and eSpCas9-GFP proteins are multi-functional enzymes with high on-target cleavage efficiency fused to Enhanced Green Fluorescent Protein (EGFP)
Protocol Guide: Immunofluorescent Staining of Whole-mount Organoids Using Antibodies
Protocol Guide: Immunofluorescent Staining of Whole-mount Organoids Using Antibodies
Duolink® In Situ Short Instructions - Fluorescence
This page details the Duolink® In Situ Short Protocol for fluorescence detection
Cell Capture Imaging Reagent
Immunocytochemistry (ICC) Cell Capture Imaging Reagent and protocol for eliminating wash steps, and is highly effective in applications involving rare cells, low cell count samples, and limited access samples.
Studies of Dimerization and Activation of Receptors in the EGFR-Family Using Duolink® Assays
Our aim was to investigate the dimerization and activation pattern of two receptors, EGFR (ErbB1) and HER2 (ErbB2), in the EGFR-family using breast cancer cell lines and selected colon tissues as model systems.
Prestige Antibodies® Organelle Markers
Subcellular localization studies are important for mapping and characterizing proteins and thus for better understanding of the cellular functions of the proteins. By confocal microscopy analysis on human cell lines, spatial and temporal protein expression patterns can be visualized on
Visikol® HISTO™ and HISTO-M™ Tissue Clearing Reagents
Visikol® HISTO™ and HISTO-M™ tissue clearing reagents for easily clearing tissues or 3D cell cultures while retaining sample integrity for numerous downstream imaging applications.
Mesenchymal Stem Cell Culture
Highly characterized human mesenchymal stem cell lines from bone-marrow, adipose and umbilical cord along with serum-free MSC expansion and differentiation media.
Human Microbiome Research Workflow: An Overview of the Role in Human Health and Disease
An overview of human microbiome research, workflow challenges, sequencing, library production, data analysis, and available microbiome reagents to support your research.
Mycoplasma Detection and Elimination
Detect mycoplasma contamination in cell cultures three ways: culture test, DNA stain test, or mycoplasma PCR test. Mycoplasma elimination kits rid cultures of contamination.
Mycoplasma Detection and Elimination In Cell Culture
Detect mycoplasma contamination in cell cultures three ways: culture test, DNA stain test, or mycoplasma PCR test. Mycoplasma elimination kits rid cultures of contamination.
Fluorescent Live Cell Imaging of Cytoskeleton Structure Proteins using LentiBrite™ Lentiviral Biosensors
High titer lentiviral particles including beta-actin, alpha-tubulin and vimentin used for live cell analysis of cytoskeleton structure proteins.
Growth Factors in Stem Cell Biology
Role of growth factors in stem cell differentiation and various growth factors for your research at sigmaaldrich.com
Live Cell Imaging Reagents
Reagents for live cell imaging are designed to be nontoxic and to protect live cells while optimizing visualization during real-time studies of cell trafficking and localization, cell health, gene expression, migration, cytoskeletal dynamics, and other cellular events.
Cell and Organelle Labeling with Fluorescent Antibodies
The Human Protein Atlas Program has carefully selected three different human cell lines, A-431 epidermoid carcinoma, U-251 MG glioblastoma and U-205 osteosarcoma, for organelle mapping of the proteome. As Prestige Antibodies are studied by immunofluorescence (IF) staining, three well-characterized organelle
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