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Duolink® PLA Fluorescence Protocol
Protocol for immunofluorescent detection of proteins in cells and tissue
Tips for Optimizing Immunofluorescence Protocols to Get the Best Image
Uses for immunofluorescence (IF)—where an antibody conjugated to a molecule that fluoresces when excited by lasers— include protein localization, confirmation of post-translational modification or activation, and proximity to/complexing with other proteins.
3D Imaging of Optically Cleared Spheroids in Corning® Spheroid Microplates
Discover how Corning® Spheroid Microplates played a key role in the use of use of 3D cell cultures for in vitro drug discovery assay.
Detection of Protein-protein Interactions by in situ PLA
Proteins are complex biological molecules essential for cellular structure and functions. The majority of proteins commonly interact with various molecules, including other proteins, in order to exert their functions.
Cell Cycle Analysis Using a Nucleoside Triphosphate (NTP) Transporter Molecule for Rapid DNA Labeling in Living Cells
Regulation of the cell cycle involves processes crucial to the survival of a cell, including the detection and repair of genetic damage as well as the prevention of uncontrolled cell division associated with cancer. The cell cycle is a four-stage
Fast and Highly Specific Histological Detection of Pathogenic Fungi in Human Tissue
The recently developed Atto-dye labeled lectins have many applications, including carbohydrate, mitogenic and histochemical studies.
Fluorescent in situ Hybridization (FISH)
Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.
How Proximity Ligation Assays (PLA) Work
Learn how Proximity Ligation Assay technology works and how the protein-protein interaction control kit can confirm in situ detection of EGF-induced EGFR-HER2 dimerization.
Studies of Dimerization and Activation of Receptors in the EGFR-Family Using Duolink® Assays
Our aim was to investigate the dimerization and activation pattern of two receptors, EGFR (ErbB1) and HER2 (ErbB2), in the EGFR-family using breast cancer cell lines and selected colon tissues as model systems.
Prestige Antibodies® Organelle Markers
Subcellular localization studies are important for mapping and characterizing proteins and thus for better understanding of the cellular functions of the proteins. By confocal microscopy analysis on human cell lines, spatial and temporal protein expression patterns can be visualized on
Human Microbiome Research Workflow: An Overview of the Role in Human Health and Disease
An overview of human microbiome research, workflow challenges, sequencing, library production, data analysis, and available microbiome reagents to support your research.
Mesenchymal Stem Cell Culture
Highly characterized human mesenchymal stem cell lines from bone-marrow, adipose and umbilical cord along with serum-free MSC expansion and differentiation media.
Mycoplasma Detection and Elimination In Cell Culture
Detect mycoplasma contamination in cell cultures three ways: culture test, DNA stain test, or mycoplasma PCR test. Mycoplasma elimination kits rid cultures of contamination.
Fluorescent Live Cell Imaging of Cytoskeleton Structure Proteins using LentiBrite™ Lentiviral Biosensors
High titer lentiviral particles including beta-actin, alpha-tubulin and vimentin used for live cell analysis of cytoskeleton structure proteins.
Mycoplasma Detection and Elimination
Detect mycoplasma contamination in cell cultures three ways: culture test, DNA stain test, or mycoplasma PCR test. Mycoplasma elimination kits rid cultures of contamination.
Fluorescent Live Cell Imaging of LC3 Mediated Autophagy using LentiBrite™ Lentiviral Biosensors
High titer lentiviral particles for LC3 variants used for live cell analysis of cellular autophagy.
Duolink® PLA Troubleshooting Guide
Support information including tips and tricks, frequently asked questions, and basic troubleshooting.
Using Immortalized 16HBE14o- Human Bronchial Epithelial Cell Lines to Model Respiratory Lung Diseases
16HBE14o- human bronchial epithelial cells used to model respiratory epithelium for the research of cystic fibrosis, viral pulmonary pathology (SARS-CoV), asthma, COPD, effects of smoking and air pollution. See over 5k publications.
Live Cell Imaging with BrightCell™ Photostable Cell Culture Media
Live cell imaging media and neuronal supplements for fluorescence microscopy using GFP, optogenetics and FACS analysis.
Growth Factors in Stem Cell Biology
Role of growth factors in stem cell differentiation and various growth factors for your research at sigmaaldrich.com
Improving Reproducibility: Best Practices for Antibodies
In the midst of beeping lab timers, presentations and grant deadlines, it is easy to take for granted the quality of lab reagents.
Duolink® Proximity Ligation Assay (PLA) Multicolor Kits for Sensitive Multiplex Detection
Duolink® PLA Multicolor technology and the quantification of protein-protein interactions in fixed tissues and cells.
Cell Culture Contamination Troubleshooting
Common Cell Culture Problems: Contamination is easily the most common problem encountered in cell culture laboratories, sometimes with very serious consequence.
Fluorescent Retinoid Probes
Fluorescent retinoic acid analogues for use in cellular imaging, protein-ligand binding assays, and flow cytometry to bypass traditional problems associated with fluorescent probes.
Tailoring Collagen-Based Matrices for Regenerative Medicine and Tissue Engineering
Collagen molecules play a critical role in tissue architecture and strength, and in cell-matrix interactions as insoluble ligands to regulate the diverse phenotypic activities of cells.
Secondary Antibodies, Conjugates, and Kits
Technical Support for Secondary Antibodies, Conjugates, and Kits
Hypoxia Detection Assays
Hypoxia detection assays to measure oxygen levels in both live and fixed cells and tissues.
Predictive Models for Neuroscience Research
Products to enable the creation of neural models specific for your research.
HumanKine® Thermostable bFGF, an engineered recombinant protein with enhanced biological functionality on human iPS and neural progenitor cells
Fibroblast growth factors (FGFs) are secreted glycoproteins that regulate several fundamental developmental pathways and help regulate mesoderm and ectoderm patterning in the early embryonic development.
Antibody Enhanced-Validation Strategies
Antibody enhanced validation provides additional data to help ensure antibody specificity and performance and to address antibody reproducibility crisis.
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