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J G Schmidt et al.
Fish & shellfish immunology, 48, 273-284 (2015-12-26)
We followed the progression of healing of deep excisional biopsy punch wounds over the course of 365 days in rainbow trout (Oncorhynchus mykiss) by monitoring visual wound healing and gene expression in the healing muscle at regular intervals (1, 3
Terri G Edwards et al.
Journal of virology, 87(7), 3979-3989 (2013-02-01)
A highly reproducible quantitative PCR (Q-PCR) assay was used to study the stability of human papillomavirus (HPV) in undifferentiated keratinocytes that maintain viral episomes. The term "stability" refers to the ability of episomes to persist with little copy number variation
Tania Nolan et al.
Nature protocols, 1(3), 1559-1582 (2007-04-05)
The real-time reverse transcription polymerase chain reaction (RT-qPCR) addresses the evident requirement for quantitative data analysis in molecular medicine, biotechnology, microbiology and diagnostics and has become the method of choice for the quantification of mRNA. Although it is often described
S A Bustin
Journal of molecular endocrinology, 29(1), 23-39 (2002-08-30)
The fluorescence-based real-time reverse transcription PCR (RT-PCR) is widely used for the quantification of steady-state mRNA levels and is a critical tool for basic research, molecular medicine and biotechnology. Assays are easy to perform, capable of high throughput, and can
Andrew J Levine et al.
Journal of neurovirology, 22(3), 366-375 (2015-12-23)
HIV infection leads to age-related conditions in relatively young persons. HIV-associated neurocognitive disorders (HAND) are considered among the most prevalent of these conditions. To study the mechanisms underlying this disorder, researchers need an accurate method for measuring biological aging. Here
Laura K MacDougall et al.
PloS one, 13(8), e0201569-e0201569 (2018-08-31)
Contaminated surfaces serve as an important reservoir for Clostridium difficile transmission. Current strategies to detect environmental contamination of C. difficile rely heavily on culture, and often only indicate presence versus absence of spores. The goal of this study was to
Ao Guo et al.
Scientific reports, 5, 14046-14046 (2015-09-16)
Regulatory T cells (Treg) play a critical role to control immune responses and to prevent autoimmunity, thus selective increase of Treg cells in vivo has broad therapeutic implications for autoimmune and inflammatory diseases. Licorice is a well-known herbal medicine used
Cawas B Engineer et al.
Plant physiology, 143(1), 236-250 (2006-11-07)
Nitrogen is an essential macronutrient for plant growth and survival. Here, the temporal and spatial sensing of nitrogen starvation is analyzed in Arabidopsis (Arabidopsis thaliana). The promoter for the high-affinity ammonium transporter, AtAmt1.1, is shown to be a valid indicator
Benjamin B Gelman et al.
Journal of acquired immune deficiency syndromes (1999), 62(5), 487-495 (2012-12-18)
Replicating HIV-1 in the brain is present in HIV encephalitis (HIVE) and microglial nodule encephalitis (MGNE) and is putatively linked with HIV-associated neurocognitive disorders (HAND). A cliniconeurovirological correlation was conducted to elucidate the relationship between brain viral load and clinical
Juan Li et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(18), 7395-7400 (2013-04-17)
Rheumatoid arthritis (RA) is an inflammatory disease in which interleukin 17 (IL-17)-producing T helper 17 (T(H)17) cells have been critically involved. We show that in patients with RA, the expression of a multifunctional regulator β-arrestin1 was significantly up-regulated in peripheral
Lithium, an anti-psychotic drug, greatly enhances the generation of induced pluripotent stem cells.
Wang, Q., et al.
Cell Research (2011)
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