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Karuppiah Muthumani et al.
Science translational medicine, 7(301), 301ra132-301ra132 (2015-08-21)
First identified in 2012, Middle East respiratory syndrome (MERS) is caused by an emerging human coronavirus, which is distinct from the severe acute respiratory syndrome coronavirus (SARS-CoV), and represents a novel member of the lineage C betacoronoviruses. Since its identification
Daniel Steil et al.
Journal of lipid research, 56(12), 2322-2336 (2015-10-16)
Shiga toxins (Stxs) are produced by enterohemorrhagic Escherichia coli (EHEC), which cause human infections with an often fatal outcome. Vero cell lines, derived from African green monkey kidney, represent the gold standard for determining the cytotoxic effects of Stxs. Despite
Kum C Hiong et al.
The Journal of experimental biology, 218(Pt 23), 3717-3728 (2015-10-10)
This study aimed to sequence and characterize two pro-coagulant genes, coagulation factor II (f2) and fibrinogen gamma chain (fgg), from the liver of the African lungfish Protopterus annectens, and to determine their hepatic mRNA expression levels during three phases of
Endothelial and lipoprotein lipases in human and mouse placenta
Lindegaard MLS, et al.
Journal of Lipid Research, 46(11), 2339-2346 (2005)
SEAP activity measurement in reporter cell-based assays using BCIP/NBT as substrate
Jerome V, et al.
Analytical biochemistry, 585, 113402-113402 (2019)
Dorota Rybaczek et al.
Histochemistry and cell biology, 135(3), 263-280 (2011-02-25)
Here, we demonstrate that in HeLa cells, Ser317 of Chk1 undergoes phosphorylation in response to replication stress induced by hydroxyurea. We also demonstrate the existence of constitutive (interphase and mitotic) Chk1 kinase phosphorylation, the translocation of its phosphorylated form from
Fernando Cartón-García et al.
Scientific reports, 5, 12312-12312 (2015-07-24)
Inherited MYO5B mutations have recently been associated with microvillus inclusion disease (MVID), an autosomal recessive syndrome characterized by intractable, life-threatening, watery diarrhea appearing shortly after birth. Characterization of the molecular mechanisms underlying this disease and development of novel therapeutic approaches
D A Knecht et al.
Analytical biochemistry, 136(1), 180-184 (1984-01-01)
A new technique for the detection of antibodies bound to proteins blotted onto nitrocellulose paper was developed. The method is rapid, sensitive, and does not require radioactive probes. Proteins transferred to nitrocellulose paper are first reacted with primary antibody followed
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