Elina Esmaeilzadeh Gharehdaghi et al.
Nucleic acid therapeutics, 24(6), 420-427 (2014-10-02)
Chitosan nanoparticles were prepared using ultrasonication methodology at specific amplitudes and times of sonication. Subsequently, small interfering RNA (siRNA) was added to the solution at predetermined values of nitrogen to phosphorous ratio (N/P), and stirring time. Employing response surfaces generated