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95729

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Hidetada Ogata et al.
The Journal of endocrinology, 222(2), 191-200 (2014-06-04)
Glucose-dependent insulinotropic polypeptide (GIP), a gut hormone secreted from intestinal K-cells, potentiates insulin secretion. Both K-cells and pancreatic β-cells are glucose-responsive and equipped with a similar glucose-sensing apparatus that includes glucokinase and an ATP-sensitive K(+) (KATP) channel comprising KIR6.2 and...
Chen Chen et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 969, 171-180 (2014-09-01)
One challenge facing the purification of therapeutic glycoproteins by affinity chromatography is creating ligands specific for the glycan moiety. Affinity chromatography of glycoproteins is currently conducted with immobilized lectins or boronates, although biomimetic ligands could present a more desirable option....
Ming-zhe Sun et al.
Biotechnology letters, 37(9), 1877-1886 (2015-05-23)
To acquire a thermostable xylanase, that is suitable for xylooligosaccharide production from pretreated corncobs, the metagenomic method was used to obtain the gene from an uncultured environmental microorganism. A thermostable xylanase-encoding gene (xyn10CD18) was cloned directly from the metagenomic DNA...
Dorit Eberhardt et al.
AMB Express, 4(1), 85-85 (2015-08-13)
L-citrulline plays an important role in human health and nutrition and is an intermediate of the L-arginine biosynthetic pathway. L-citrulline is a by-product of L-arginine production by Corynebacterium glutamicum. In this study, C. glutamicum was engineered for overproduction of L-citrulline...
Fátima Vargas et al.
Bioresource technology, 191, 263-270 (2015-05-23)
In the present work, the hydrothermal valorization of an abundant agricultural residue has been studied in order to look for high added value applications by means of hydrothermal pretreatment followed by fed-batch simultaneous saccharification and fermentation, to obtain oligomers and...
Nan Wang et al.
Carbohydrate polymers, 111, 47-55 (2014-07-20)
A neutral polysaccharide fraction (SMPA) prepared from the roots of Salvia miltiorrhiza by DEAE-cellulose and Sephadex G-100 chromatography was tested for its immune enhancing function in N-methyl-N'-nitro-nitrosoguanidine (MNNG)-induced gastric cancer rats by intragastric administration. SMPA (200mg/kg) treatment not only increased...
Pengfei Sun et al.
Scientific reports, 5, 17465-17465 (2015-12-03)
In the present work, a novel bioflocculant, EPS-1, was prepared and used to flocculate the kaolin suspension and Microcystis aeruginosa. We focused on the characteristics and flocculation performance of EPS-1, especially with regard to its protein components. An important attribute...
Wellington Muchero et al.
BMC genomics, 16, 24-24 (2015-01-24)
QTL cloning for the discovery of genes underlying polygenic traits has historically been cumbersome in long-lived perennial plants like Populus. Linkage disequilibrium-based association mapping has been proposed as a cloning tool, and recent advances in high-throughput genotyping and whole-genome resequencing...
Licia Lama et al.
AMB Express, 4, 55-55 (2014-07-16)
Polysaccharidases from extremophiles are remarkable for specific action, resistance to different reaction conditions and other biotechnologically interesting features. In this article the action of crude extracts of thermophilic microorganisms (Thermotoga neapolitana, Geobacillus thermantarcticus and Thermoanaerobacterium thermostercoris) is studied using as...
Leonora Rios de Souza Moreira et al.
Carbohydrate polymers, 134, 700-708 (2015-10-03)
Two endo-β-1,4-xylanases named XylT1 and XylT2, previously purified from Aspergillus terreus, were structurally investigated by fluorescence quenching and characterized with respect to their binding properties with phenolic compounds. Neutral and charged quenchers had access to both enzymes in neutral and...
Yun Li et al.
Bioresource technology, 200, 272-278 (2015-10-27)
This study investigated the effect of alkali lignins with different molecular weights on enzymatic hydrolysis of lignocellulose. Different alkali lignins fractions, which were obtained from cascade ultrafiltration, were added into the dilute acid pretreated (DAP) and alkali pretreated (AP) rice...
Azam Fatahi Sadeghabadi et al.
Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences, 19(Suppl 1), S51-S55 (2014-07-09)
Antibiotic resistance of enteric pathogens particularly Shigella species, is a critical world-wide problem and monitoring their resistant pattern is essential, because the choice of antibiotics is absolutely dependent on regional antibiotic susceptibility patterns. During summer 2013, an unusual increase in...
Meigui Huang et al.
Journal of food science and technology, 52(2), 858-866 (2015-02-20)
A novel gradient temperature-elevating Maillard reaction was developed to effectively produce light-colored MRPs. The main purpose of the present study was to compare the color, taste characteristic, non-volatile compounds, volatile compounds and antioxidant activity of MRPs prepared by the novel...
Huiling Li et al.
Bioresource technology, 176, 242-248 (2014-12-03)
The conversion of xylose, water-insoluble hemicelluloses (WIH) and water-soluble fraction (WSF) of corncob to furfural was performed using montmorillonite with tin ions (Sn-MMT) containing double acid sites as a solid acid catalyst. The co-existence of Lewis acids and Brønsted acids...
Dong-Dong Meng et al.
Applied and environmental microbiology, 81(6), 2006-2014 (2015-01-13)
Xylanases are crucial for lignocellulosic biomass deconstruction and generally contain noncatalytic carbohydrate-binding modules (CBMs) accessing recalcitrant polymers. Understanding how multimodular enzymes assemble can benefit protein engineering by aiming at accommodating various environmental conditions. Two multimodular xylanases, XynA and XynB, which...
Tong-Chao Su et al.
Scientific reports, 5, 17538-17538 (2015-12-10)
In this study, magnetic carbonaceous acids were synthesized by pyrolysis of the homogeneous mixtures of glucose and magnetic Fe3O4 nanoparticles, and subsequent sulfonation. The synthesis conditions were optimized to obtain a catalyst with both high acid density (0.75 mmol g(-1))...
Tian Xia et al.
Applied and environmental microbiology, 81(10), 3387-3394 (2015-03-10)
Escherichia coli that is unable to metabolize d-glucose (with knockouts in ptsG, manZ, and glk) accumulates a small amount of d-glucose (yield of about 0.01 g/g) during growth on the pentoses d-xylose or l-arabinose as a sole carbon source. Additional...
Fei Huang et al.
Molecules (Basel, Switzerland), 19(8), 12760-12776 (2014-08-21)
Dried litchi pulp has been used in traditional remedies in China for many years to treat various diseases, and the therapeutic activity has been, at least partly, attributed to the presence of bioactive polysaccharides. Polysaccharide-protein complexes from vacuum freeze-(VF), vacuum...
Benjamin L Golomb et al.
Journal of bacteriology, 197(2), 371-381 (2014-11-12)
Lactic acid bacteria have been isolated from living, harvested, and fermented plant materials; however, the adaptations these bacteria possess for growth on plant tissues are largely unknown. In this study, we investigated plant habitat-specific traits of Lactococcus lactis during growth...
Belisa B de Sales et al.
Biotechnology letters, 37(10), 1973-1982 (2015-06-20)
Since uptake of xylose limits its fermentation, we aimed to identify novel sugar transporters from Scheffersomyces stipitis that allow xylose uptake and fermentation by engineered Saccharomyces cerevisiae. An hxt-null S. cerevisiae strain, lacking the major hexose transporters (hxt1Δ-hxt7Δ and gal2Δ)...
Rongjun He et al.
International journal of biological macromolecules, 78, 56-61 (2015-04-04)
Three polysaccharides (SAP30, SAP60 and SAP80) were separated from the body of Edwardsia sipunculoides by tissue homogenate and papain hydrolysis. Total soluble sugar contents, monosaccharide compositions, antioxidant and antitumor activities in vitro of those polysaccharides were investigated, respectively. Results showed...
Stephen R Hughes et al.
Journal of laboratory automation, 20(6), 621-635 (2015-02-28)
A yeast artificial chromosome (YAC) containing a multigene cassette for expression of enzymes that enhance xylose utilization (xylose isomerase [XI] and xylulokinase [XKS]) was constructed and transformed into Saccharomyces cerevisiae to demonstrate feasibility as a stable protein expression system in...
Joseph A Rollin et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(16), 4964-4969 (2015-04-08)
The use of hydrogen (H2) as a fuel offers enhanced energy conversion efficiency and tremendous potential to decrease greenhouse gas emissions, but producing it in a distributed, carbon-neutral, low-cost manner requires new technologies. Herein we demonstrate the complete conversion of...
Wenjian Guan et al.
Bioresource technology, 200, 713-721 (2015-11-13)
Paper mill sludge (PS), a solid waste from pulp and paper industry, was investigated as a feedstock for acetone-butanol-ethanol (ABE) production by simultaneous saccharification and fermentation (SSF). ABE fermentation of paper sludge by Clostridium acetobutylicum required partial removal of ash...
Jose Serate et al.
Biotechnology for biofuels, 8, 180-180 (2015-11-20)
Microbial conversion of lignocellulosic feedstocks into biofuels remains an attractive means to produce sustainable energy. It is essential to produce lignocellulosic hydrolysates in a consistent manner in order to study microbial performance in different feedstock hydrolysates. Because of the potential...
Legesse Garedew et al.
BMC microbiology, 15, 100-100 (2015-05-13)
Listeriosis, mostly caused by Listeria monocytogenes species, has become a major concern to public health authorities due to its clinical severity and high mortality rate, particularly in high risk groups. Currently, there is limited information regarding the prevalence and antimicrobial...
Alexandre F Somera et al.
Applied and environmental microbiology, 81(13), 4525-4535 (2015-04-26)
Leaf-cutter ants use plant matter to culture the obligate mutualistic basidiomycete Leucoagaricus gongylophorus. This fungus mediates ant nutrition on plant resources. Furthermore, other microbes living in the fungus garden might also contribute to plant digestion. The fungus garden comprises a...
Jin Qi et al.
Chemical & pharmaceutical bulletin, 63(3), 187-194 (2015-03-12)
Two new steroidal glycosides (1 and 2), together with 15 known compounds (3-17) were isolated from the fibrous roots of Ophiopogon japonicus, and three new steroidal glycosides (18-20), together with 14 known compounds (21-34) were isolated from the fibrous roots...
Huijun Wang et al.
Carbohydrate polymers, 124, 98-108 (2015-04-04)
A water-soluble polysaccharide, 7WA, with an average molecular mass of 7.1×10(4)Da, was isolated from the leaves of green tea. Monosaccharide composition analysis indicated that 7WA mainly contained Arabinose and Galactose in the molar ratio of 1.0:0.96. By using the methods...
Philippe Debeire et al.
FEMS microbiology letters, 355(2), 116-123 (2014-05-16)
Fusarium graminearum was grown on four lignocellulosic substrates (corn cobs, wheat bran, hop cell walls, and birchwood) and glucose as the sole carbon source. Proteomic studies performed on the resulting enzymatic cocktails highlighted a great diversity in the number and...
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