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Quantitative real-time reverse transcription-PCR analysis reveals stable and prolonged neurotoxin cluster gene activity in a Clostridium botulinum type E strain at refrigeration temperature.

Ying Chen et al.

Applied and environmental microbiology, 74(19), 6132-6137 (2008-08-19)

The relative expression levels of six botulinum neurotoxin cluster genes in a group II Clostridium botulinum type E strain grown at 10 or 30 degrees C were investigated using quantitative real-time reverse transcription-PCR. An enzyme-linked immunosorbent assay was used to

Profiling the transcriptome with RNA SPOTs.

Chee-Huat Linus Eng et al.

Nature methods, 14(12), 1153-1155 (2017-11-14)

Single-molecule FISH (smFISH) has been the gold standard for quantifying individual transcript abundances. Here, we scale up multiplexed smFISH to the transcriptome level and profile 10,212 different mRNAs from mouse fibroblast and embryonic stem cells. This method, called RNA sequential

Rapid method for the detection of genetically engineered microorganisms by polymerase chain reaction from soil and sediments.

A A Khan et al.

Journal of industrial microbiology & biotechnology, 20(2), 90-94 (1998-06-27)

A rapid and sensitive method for the detection of genetically engineered microorganisms in soil and sediments has been devised by in vitro amplification of the target DNAs by a polymerase chain reaction. A cloned catechol 2,3-dioxygenase gene located on the

Evaluation of the pH- and thermal stability of the recombinant green fluorescent protein (GFP) in the presence of sodium chloride.

Marina Ishii et al.

Applied biochemistry and biotechnology, 137-140(1-12), 555-571 (2008-05-15)

The thermal stability of recombinant green fluorescent protein (GFP) in sodium chloride (NaCl) solutions at different concentrations, pH, and temperatures was evaluated by assaying the loss of fluorescence intensity as a measure of denaturation. GFP, extracted from Escherichia coli cells

Comparison of phage pVIII and KLH as vector in inducing the production of cytokines in C57BL/6J mice.

Quan-Ping Su et al.

Vaccine, 25(6), 970-975 (2006-10-24)

We have demonstrated that phage display Candida albicans (C. albicans) LKVIRK epitope was protective in systemically infected C57BL/6J mice. The different development from precursor Ths, Th1 or Th2, will result in a protective or nonprotective immune response. To compare the

In vivo antigen-driven plasmablast enrichment in combination with antigen-specific cell sorting to facilitate the isolation of rare monoclonal antibodies from human B cells.

Zhonghua Lin et al.

Nature protocols, 9(7), 1563-1577 (2014-06-06)

The ability to rapidly generate large panels of antigen-specific human antibodies in a rodent would enable the efficient discovery of novel therapeutically useful antibodies. We have developed a system wherein human antigen-specific antibody-secreting plasmablasts can be enriched in vivo, in

Multimodal profiling of single-cell morphology, electrophysiology, and gene expression using Patch-seq.

Cathryn R Cadwell et al.

Nature protocols, 12(12), 2531-2553 (2017-12-01)

Neurons exhibit a rich diversity of morphological phenotypes, electrophysiological properties, and gene-expression patterns. Understanding how these different characteristics are interrelated at the single-cell level has been difficult because of the lack of techniques for multimodal profiling of individual cells. We

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